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Method for producing anti-apolipoprotein A1 (ApoA1) multi-antibody serum by using Zhejiang sheep

A technology of serum and Hu sheep, applied in the direction of serum immunoglobulin, anti-animal/human immunoglobulin, etc., can solve the problems of low yield and potency, unable to meet the use requirements, etc., and achieve high-efficiency results

Inactive Publication Date: 2013-12-25
ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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AI Technical Summary

Problems solved by technology

It is an important raw material for biological reagents. The existing production method adopts the traditional immunization method, and the yield and potency are low, which can no longer meet the growing demand for use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Example: 50 Hu sheep rams were used as experimental animals, healthy sheep with a body weight of about 35 kilograms. Before immunization, all ear tags were put on. Automatic drinking fountains, daily pasture supply, appropriate addition of concentrate, vinegar grains, bran and vitamins, etc.

[0020] Human APOB was inoculated with 0.1ml (combined antigen 25mg) and 1.9ml of sterile PBS with complete Freund's (or incomplete) adjuvant 2ml to make immunogen emulsion for later use.

[0021] The first injection of Hu sheep immunization, front and rear groin, each groin is divided into 2 areas for injection, a total of 12 injection points, subcutaneous injection, 2 points on both sides of the neck are injected equally.

[0022] The second injection, with an interval of ten days, was injected in two areas in the front and rear groin, a total of 12 injection points, and two injection points on the left and right of the lymph nodes on both sides of the neck.

[0023] The third i...

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PUM

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Abstract

The invention discloses a method for producing anti-apolipoprotein A1 (ApoA1) multi-antibody serum by using a Zhejiang sheep. The method comprises the following steps: preparing immunogenic original emulsion, selecting the Zhejiang sheep to perform immunization for seven times, and the like, wherein the groin and the neck of the Zhejiang sheep are selected to inject during the immunization; finally, collecting the blood and separating the serum. The titer of the multi-antibody serum which is finally produced by the production method is 1:16; the multi-antibody serum has high benefit.

Description

technical field [0001] The invention relates to an antigen production method, in particular to a method for producing anti-ApoA1 polyantiserum by using Hu sheep and a method for using the same. Background technique [0002] Apolipoprotein A1 (ApoA1) is the most abundant component of the apoA family. ApoA1 is a single polypeptide chain consisting of 243 amino acid residues with a molecular weight of 28300D. It is the main apolipoprotein of high-density lipoprotein (HDL). It is an important raw material of biological reagents. The existing production method adopts the traditional immunization method, and the yield and potency are low, which can no longer meet the growing demand for use. Contents of the invention [0003] Purpose of the invention: In order to overcome the deficiencies of the above-mentioned prior art, the present invention provides a method for producing anti-ApoA1 polyantiserum by using Hu sheep, which can increase the yield and titer. [0004] Technical so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/06
Inventor 徐志伟
Owner ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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