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Biosensor based on up-converting phosphor technology

A biosensor, technology, applied in instruments, scientific instruments, fluorescence/phosphorescence, etc., can solve the problems of spectral interference of non-dispersive optical systems, unable to obtain measurement results of elements, etc., and achieve the effect of sensitive qualitative and quantitative detection and analysis

Inactive Publication Date: 2013-12-18
凌中鑫
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Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the existing atomic fluorescence instruments are non-dispersive spectrometers. Due to the problem of spectral interference in the non-dispersive optical system, it is impossible to obtain accurate measurement results for some elements, and to provide a technology based on up-conversion luminescence biosensor

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  • Biosensor based on up-converting phosphor technology
  • Biosensor based on up-converting phosphor technology

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Embodiment Construction

[0014] The present invention will be described in further detail below in conjunction with the accompanying drawings: the present embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation is provided, but the protection scope of the present invention is not limited to the following embodiments.

[0015] Such as figure 2 As shown, a biosensor based on up-transfer luminescence technology involved in this embodiment includes: an excitation optical path module, a photoelectric signal receiving and converting module, a control system module, an amplification and shaping circuit 28, a decoding interface circuit 29 and a data buffer module 30. The excitation light path module includes a main detection excitation light module 1 and an auxiliary optical scanning module 2; the photoelectric signal receiving conversion module includes a main detection photoelectric conversion module 3 and an auxiliary scanning photoelectric c...

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Abstract

The invention provides a biosensor based on the up-converting phosphor technology and application of the biosensor. The biosensor comprises an exciting light path module, a photoelectric signal receiving and converting module, a control system module, an amplification shaping circuit, a coding interface circuit and a data-caching module, wherein the exciting light path module comprises a main detection exciting light module and an auxiliary optical scanning module; the photoelectric signal receiving and converting module comprises a main detection photoelectric converting module and an auxiliary scanning photoelectric converting module. The biosensor adopts a phosphor material (platinum / palladium porphyrin) as a biomarker, and a result is shown in an infrared light signal form under the green light irradiation, and can be interpreted by an instrument, so that the quantitative detection of the target substance to be detected is realized.

Description

technical field [0001] The invention relates to a biosensor based on up-transfer luminescence technology and its application, belonging to the technical field of immune detection. Background technique [0002] Phosphorescence analysis is the sister technology of fluorescence analysis. Compared with fluorescence, it has many unique advantages: (1) It has a large Stokes shift, the wavelength of phosphorescence is longer than that of fluorescence, and it is far away from the excitation spectrum , will not overlap with the excitation spectrum, not only can reduce or eliminate the interference of the background fluorescence of the sample (especially biological samples) and the incident excitation light, but also reduce the self-absorption phenomenon; (2) due to T 1 →S 0 Spin forbidden, the lifetime of phosphorescence is longer than that of fluorescence, and the lifetime of phosphorescence is about 10 -3 -10s, easy to achieve time-resolved determination; (3) better selectivity. ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/532
Inventor 凌中鑫
Owner 凌中鑫
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