Preparation method of sulfur-allyl-L-cysteine

A technology of cysteine ​​and allyl group, applied in the field of bioengineering, can solve the problems of complex production process and low yield, and achieve the effect of simple preparation process, high production efficiency and short time

Inactive Publication Date: 2013-12-18
SICHUAN XICHENG NATURAL FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the low yield of the prior art, the deficiencies of complex production technology, to provide a gamma -Glutamyl-sulfur-allyl-L-cysteine ​​is a raw material, a method for biosynthesizing sulfur-allyl-L-cysteine

Method used

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  • Preparation method of sulfur-allyl-L-cysteine
  • Preparation method of sulfur-allyl-L-cysteine
  • Preparation method of sulfur-allyl-L-cysteine

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A preparation method of sulfur-allyl-L-cysteine, comprising the steps of:

[0033] Take 60.6g gamma -glutamyl-thio-allyl- L - Cysteine, dissolved in 10 L of NH with a concentration of 0.05 mol / L and a pH of 9.5 3 -NH 4 Add 0.1L of γ-glutamyl transpeptidase solution (specific activity 500U / mL) to the Cl buffer solution, stir and react at 37°C for 60 minutes, and measure the sulfur-allyl-L-cysteine ​​in the solution after the reaction The concentration was 2.74g / L, and the yield of sulfur-allyl-L-cysteine ​​was 85.1%.

Embodiment 2

[0035] A preparation method of sulfur-allyl-L-cysteine, comprising the steps of:

[0036] The purified γ-glutamyl transpeptidase was embedded in chitosan and cross-linked with glutaraldehyde to obtain immobilized γ-glutamyl transpeptidase, and its activity was determined to be 53U / g; 606g gamma -glutamyl-thio-allyl- L -Cysteine ​​is dissolved in 100L of NH with a concentration of 0.05mol / L and a pH of 10.0 3 -NH 4 In Cl buffer solution, add 500 g of immobilized γ-glutamyl transpeptidase to the buffer solution, stir and react at 37°C for 60 minutes to obtain a reaction solution; filter and recover the immobilized γ-glutamyl transpeptidase in the reaction solution , the concentration of sulfur-allyl-L-cysteine ​​in the remaining reaction solution was detected to be 2.64g / L, and the yield of sulfur-allyl-L-cysteine ​​was 82.0%.

Embodiment 3

[0038] A preparation method of sulfur-allyl-L-cysteine, comprising the steps of:

[0039] The purified γ-glutamyl transpeptidase was embedded in alginate and cross-linked with anhydrous butylene dioic acid-ethylene to obtain immobilized γ-glutamyl transpeptidase, and its activity was determined to be 85 U / mg; Take 1212g gamma -glutamyl-thio-allyl- L - Cysteine ​​dissolved in 100L of NH with a concentration of 0.05mol / L and a pH of 9.0 3 -NH 4 In Cl buffer solution, stir and react at 37°C for 120 minutes to obtain a reaction solution; filter and recover the immobilized γ-glutamyl transpeptidase in the reaction solution, and detect sulfur-allyl-L-cysteine ​​in the remaining reaction solution The acid concentration was 5.16g / L, and the yield of sulfur-allyl-L-cysteine ​​was 80.1%.

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Abstract

The invention discloses a preparation method of sulfur-allyl-L-cysteine. The preparation method comprises the following steps of dissolving gamma-glutamoyl-sulfur-allyl-L-cysteine in a buffer solution, and adding gamma-glutamyl transpeptidase as a catalyst to the buffer solution for reaction so as to obtain a reaction liquid containing the sulfur-allyl-L-cysteine; sending the reaction liquid to a chromatographic column for adsorption separation, washing with de-ionized water and eluting by using a 0.3 mol / L-1.2 mol / L ammonia solution, and collecting an eluant containing the sulfur-allyl-L-cysteine; adding a solvent after the eluant is subjected to evaporation and concentration, stewing at a room temperature and separating out crystals; washing the crystals with ethanol, and recrystallizing by using an ethanol solution with the volume fraction of 60% after the crystals are dried so as to obtain the crystals containing the sulfur-allyl-L-cysteine. The preparation method of the sulfur-allyl-L-cysteine has the advantages that preparing the sulfur-allyl-L-cysteine is prepared in a high yield and the problem that the sulfur-allyl-L-cysteine is oxidized into alliin by oxidase in garlic cells is avoided.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method of thio-allyl-L-cysteine. Background technique [0002] Sulfur-allyl-L-cysteine ​​(S-allyl-l-cysteine, SAC) is a water-soluble non-protein amino acid in garlic. [0003] The research results show that SAC has the functions of antibacterial, anti-oxidation, enhancing the activity of SOD in the body, and anti-amyloid protein, and is used for the prevention and treatment of various diseases such as Alzheimer's disease, heart disease, and liver disease. At present, the SAC used in medical research is mainly derived from fermented black garlic (Aged garlic) extract, but the SAC content in black garlic is very low, so it is not suitable as a raw material. [0004] Chinese patent CN102077965A discloses a method for preparing black garlic and extracting active substances therefrom. The garlic is placed in an environment with a temperature of 60-80°C and a relative humi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/12
Inventor 刘福锦包塔娜苏辉黄俐唐宁曾艳丽任建波
Owner SICHUAN XICHENG NATURAL FOODS
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