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Aureobasidium pullulans OF-01 strain and method for preparing tobacco endogenous derived spice by using strain

An endogenous technology of Aureobasidium pullulans, which is applied in the preparation of tobacco, microbial-based methods, botany equipment and methods, etc., can solve the problems of no Aureobasidium pullulans strains, and achieve standardization and controllability Strong sex, improved taste, and increased utilization

Active Publication Date: 2013-12-18
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of Aureobasidium pullulans strain OF-01 isolated from Aureobasidium pullulans

Method used

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  • Aureobasidium pullulans OF-01 strain and method for preparing tobacco endogenous derived spice by using strain
  • Aureobasidium pullulans OF-01 strain and method for preparing tobacco endogenous derived spice by using strain
  • Aureobasidium pullulans OF-01 strain and method for preparing tobacco endogenous derived spice by using strain

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Experimental program
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Embodiment 1

[0023] 1. Source of microorganism OF-01 strain of the present invention:

[0024] Pulverize the sample of Honghua Dajinyuan tobacco leaf that has been stored and aged for 3 years, put 5 grams of pulverized tobacco powder into a triangular flask filled with 100ml of sterile water, shake it on a shaker at 100-250rpm for 30 minutes, and then filter to remove the tobacco. At the end, use the suspension to coat MYP medium (malt extract 7g, yeast extract 0.5g, peptone 2.5g, agar 20g, water 1L, pH7.0) plate, 200 microliters / dish, a total of 50 dishes, at 28 After culturing in an incubator at ℃ for 5-7 days, a single microorganism was purified with MYP medium, and the OF-01 strain with aroma-producing properties was screened from the purified microorganisms on the surface of tobacco leaves, which was identified as Aureobasidium pullulans .

[0025] Aureobasidium pullulans (Aureobasidium pullulans) is a yeast-like fungus with yeast-type and fungal-type mycelium forms. According to its...

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Abstract

The invention discloses an aureobasidium pullulans OF-01 strain (CGMCC No.7609) (China General Microbiological Culture Collection Center Number 7609), a method for preparing a tobacco endogenous derived spice by using the strain, and an application of the strain in a tobacco additive. A tobacco leaf fragment is used as a raw material, the aureobasidium pullulans OF-01 strain is used for fermentation, a plurality of products, such as extracellular polysaccharides (pullulan), enzymes, antibiotics, single-cell protein, volatile aroma components and the like, are generated, and the fermented tobacco leaf fragment is extracted and condensed to prepare the tobacco endogenous derived spice, therefore, the application of the spice and flavor products which are not derived from the tobacco in cigarette is reduced to achieve the re-utilization of the tobacco leaf fragment. The analysis on the aroma components proves that the spice comprises a plurality of types of tobacco aroma components and other derived aroma components. The result of a flavoring smoke panel test proves that the spice can be used for obviously increasing cigarette flavor, causing the smoke pure and mild, reducing the irritation, covering the offensive odor, and improving the cigarette smell. The spice has the characteristics of low cost, rich aroma components, safety to people and livestock and the like, therefore, the spice can be used in the production of the cigarette.

Description

technical field [0001] The invention belongs to the technical field of preparation of tobacco additives, and in particular relates to a strain of Aureobasidium pullulans OF-01 and a method for preparing tobacco endogenously derived spices by using it, and also relates to its application in cigarettes. Background technique [0002] Tobacco flavoring is an additive specially used for flavoring and flavoring various tobacco products. Tobacco flavors are mostly artificial blends containing two or more aroma components, which are generally divided into natural flavors and synthetic flavors (Yu Jinheng et al., 2011). Natural spices can be derived from plants, animals, and microorganisms. According to different extraction methods, different products such as essential oils, extracts, absolutes, balsams, and tinctures can be obtained. Natural tobacco flavors can release aroma and fragrance after burning and cracking after being added to cigarettes, which occupies a very important po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/31C11B9/00A24B3/12C12R1/645
Inventor 杨蕾杨清杨乾栩段焰青何雪峰李勇蔡波党立志师建全杨洪明周国福冯洪涛莫明和
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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