Method for Aspergillus oryzae to degrade straw to produce protein feed

Abstract

Relating to the field of biological engineering, the invention provides a method for Aspergillus oryzae to degrade straw to produce protein feed. The method uses straw as a main raw material to produce protein feed by Aspergillus oryzae fermentation. The production process includes: subjecting the straw to crushing, pre-wetting and other pre-treatment technologies, and subjecting the Aspergillus oryzae to liquid shaking culture, first class seed culture, and mixing with straw to undergo solid state fermentation, thus obtaining fermented straw protein feed. The fermentation process is simple, has no pollution to the environment, and also causes no resource waste. The feed prepared by the method has a protein content of 15-30%, a lignin degradation rate of 34-76%, and a feed yield of 45-60%. Because of the effects of Aspergillus oryzae, the feed can degrade lignin and cellulose, thus improving the utilization rate of straw.

Description

technical field [0001] The invention relates to the field of bioengineering, and in particular refers to a process for producing protein feed by degrading lignin and cellulose in the straw without high temperature and high pressure treatment by Aspergillus oryzae. Background technique [0002] my country is a large agricultural country, with an annual output of more than 600 million tons of crop straw, except for a small amount used in the textile and papermaking industries, but most of it is dumped into the environment in the form of accumulation and incineration, which wastes resources and causes great harm to the environment . [0003] Straw contains more than half of the energy accumulated by plant photosynthesis. It is rich in raw sugar, crude protein and other ingredients that are beneficial to livestock and poultry. As a non-competitive feed resource, as long as it can be processed and prepared reasonably, it can be used to feed livestock and poultry. Poultry, especi...

AI Technical Summary

Problems solved by technology

Steam explosion is to use high-pressure steam to infiltrate the interior of the fiber and release it from the closed space in the form of air flow. The fiber undergoes a certain mechanical fracture, and most of the hemicellulose and a small amount of lignin and cellulose in the raw material are degraded and dissolved. The composition of the method is relatively high, large-scale equipment is required, and lignin needs to be treated; the acid treatment method uses sulfuric acid to hydrolyze glycosidic bonds, ester bonds, etc., so that the network structure of corn stalk fibers is loose, but this method consumes a large amount of organic acid or Inorganic acid and lignin still need to be further processed and are not suitable for large-scale industrial production

Alkali treatment and ammonia water treatment use lignin to dissolve in alkaline solution, and there are also environmental pollution problems like acid treatment

This is due to the fact that these methods generally have problems such as difficult control of reaction conditions, high reaction temperature, long treatment time, large space occupation, unsatisfactory treatment effect, and inability to remove lignocellulose.

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