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A kind of rapid propagation method of golden nanmu tissue culture

A technology of golden nanmu and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve problems such as being unsuitable for golden nanmu cultivation.

Inactive Publication Date: 2016-01-20
ZUNYI HUAFU AGRI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This program is suitable for the tissue culture of Photinia rosacea, because Photinia rosacea is a plant of the family Rosaceae, and its biological tissue characteristics and growth requirements are different from those of Photinia lauraceae, so this program is not suitable for the cultivation of Photinia chinensis.

Method used

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  • A kind of rapid propagation method of golden nanmu tissue culture
  • A kind of rapid propagation method of golden nanmu tissue culture
  • A kind of rapid propagation method of golden nanmu tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] (1) Seed selection, pretreatment, soaking and germination in the substrate, transfer culture after embryo differentiation:

[0065] Select full-bodied and uninjured Phoebe seeds, pretreat them with 800 times carbendazim solution, then soak the seeds in 20ppm rooting agent for 30 minutes and put them into the perlite matrix, with a size of 4cm×4cm Place them at a certain distance, and then spray water to wet them. When the seeds germinate and the embryos are differentiated and there are small roots growing, then move the seeds to a sterilized seedbed for cultivation to obtain young tender seedlings and young tender shoots;

[0066] (2) Take newborn tender seedlings and cut them into sections for domestication and then carry out rooting culture:

[0067] Take the young seedlings, wash them with running water for half an hour, cut them into 1.5cm long stems with 2 nodes, disinfect the surface with 0.15% mercuric chloride solution for 15 minutes, wash them with sterile wate...

Embodiment 2

[0079] (1) Seed selection, pretreatment, soaking and germination in the substrate, transfer culture after embryo differentiation:

[0080] Select full-bodied and uninjured Phoebe seeds, pretreat them with 800 times carbendazim solution, then soak the seeds in 20ppm rooting agent for 30 minutes and put them into the perlite matrix, with a size of 4cm×4cm Place them at a certain distance, and then spray water to wet them. When the seeds germinate and the embryos are differentiated and there are small roots growing, then move the seeds to a sterilized seedbed for cultivation to obtain young tender seedlings and young tender shoots;

[0081] (2) Take newborn tender seedlings and cut them into sections for domestication and then carry out rooting culture:

[0082] Take the young seedlings, wash them with running water for half an hour, cut them into 1.5cm long stems with 2 nodes, disinfect the surface with 0.15% mercuric chloride solution for 15 minutes, wash them with sterile wate...

Embodiment 3

[0093] (1) Seed selection, pretreatment, soaking and germination in the substrate, transfer culture after embryo differentiation:

[0094] Select full-bodied and uninjured Phoebe seeds, pretreat them with 800 times carbendazim solution, then soak the seeds in 20ppm rooting agent for 30 minutes and put them into the perlite matrix, with a size of 4cm×4cm Place them at a certain distance, and then spray water to wet them. When the seeds germinate and the embryos are differentiated and there are small roots growing, then move the seeds to a sterilized seedbed for cultivation to obtain young tender seedlings and young tender shoots;

[0095] (2) Take newborn tender seedlings and cut them into sections for domestication and then carry out rooting culture:

[0096] Take the young seedlings, wash them with running water for half an hour, cut them into 1.5cm long stems with 2 nodes, disinfect the surface with 0.15% mercuric chloride solution for 15 minutes, wash them with sterile wate...

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Abstract

The invention relates to a rapid propagation method for tissue culture of phoebe zhennan. The rapid propagation method comprises the following steps: (1) selecting seeds; pre-treating the seeds and immersing the seeds in a base material for germination; after embryos are differentiated, transferring and culturing; (2) carrying out bud induction culture by taking tender coppice shoots as explants; (3) cutting the newborn tender seedlings into sections and carrying out rooting culture; (4) inoculating adventitious root buds into a proliferation culture medium and carrying out proliferation culture; (5) inoculating the adventitious root buds obtained by the proliferation culture into a rooting culture medium and carrying out rooting culture; (6) domesticating and transplanting. According to the rapid propagation method, the rooting culture of seedling stem section tissues is combined with adventitious root bud induction proliferation culture to reasonably domesticate and circularly take materials, so as to keep complete genomes of the phoebe zhennan, improve the survival rate and the quality of the phoebe zhennan and increase the growing speed of the phoebe zhennan.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method for tissue culture of nanmu. Background technique [0002] Golden nanmu (scientific name: phoebezhennanS.LeeetF.N.Wei) is an evergreen tree of Lauraceae, a national second-class protected endangered species. Greening tree species are mainly distributed in western Hubei, northwestern Guizhou and Sichuan in China. According to "Bogu Yaolan", there are three kinds of nanmu, namely Xiangnan, Jinsinanmu and Shuinan. Among them, golden nanmu is the most precious, which belongs to the national second-class protected plant and was on the verge of extinction in the late Ming Dynasty. The material of golden nanmu has the characteristics of moisture resistance, corrosion resistance, no deformation, mild fragrance, warm in winter and cool in summer, and it will emit golden light under the light. Since ancient times, golden nanmu has been an importan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵光平
Owner ZUNYI HUAFU AGRI BIOLOGICAL SCI & TECH CO LTD
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