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Novel method for extracting rutin from pagodatree flower bud

A new method, the technology of Huai Mi, applied in the field of phytochemistry, can solve the problems of not meeting the quality standards of raw materials, poor extract purity, and low rutin extraction rate, and achieve the effects of low production cost, improved solubility, and accelerated dissolution

Inactive Publication Date: 2013-12-11
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods all have disadvantages such as high energy consumption and long extraction time; the alkali-dissolving acid precipitation method is simple to operate, low in energy consumption, and is suitable for industrial production requirements, but the extraction rate of rutin is low; Poor purity, unable to meet raw material quality standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Superfinely pulverize Huai Mi, take 100g of crushed Huai Mi, add a sodium carbonate solution with a pH value of 8 that is 9 times the weight of the raw material, conduct continuous countercurrent extraction at a temperature of 40°C, a fixed ultrasonic frequency of 20KHz, and a power of 90W for 50 minutes, and then filter. Repeat the extraction twice, combine the filtrates, add sulfuric acid to the obtained extract, adjust the pH value to 4, let it stand for 24 hours, filter with suction, add 2 times the amount of methanol to the obtained crude extract to dissolve it completely, and then Stir with distilled water at 90°C with 8 times the volume of the crude extract, cool down naturally until the rutin recrystallizes, repeat the crystallization twice, and then filter and dry the obtained crystals to obtain 14.2 g of high-purity rutin with a purity of 98.8%.

Embodiment 2

[0017] Superfinely pulverize Huai Mi, take 100g of crushed Huai Mi, add lime milk solution with a pH value of 9 that is 10 times the weight of the raw material, conduct continuous countercurrent extraction at a temperature of 45°C, a fixed ultrasonic frequency of 20KHz, and a power of 110W for 40 minutes, and then filter. Repeat the extraction twice, combine the filtrates, add hydrochloric acid to the obtained extract, adjust the pH value to 3, let it stand for 24 hours, filter with suction, add 3 times the amount of methanol to the obtained crude extract to dissolve it completely, and then Stir with distilled water at 90°C with 8 times the volume of the crude extract, cool down naturally until the rutin recrystallizes, repeat the crystallization twice, then filter and dry the obtained crystals to obtain 15.2 g of high-purity rutin with a purity of 99.2%.

Embodiment 3

[0019] Superfinely pulverize Huai Mi, take 100g of pulverized Huai Mi, add a sodium hydroxide solution with a pH value of 10 that is 11 times the weight of the raw material, conduct continuous countercurrent extraction at a temperature of 50°C, a fixed ultrasonic frequency of 20KHz, and a power of 120W for 20 minutes and then filter. Repeat the extraction of the filter residue twice, combine the filtrates, add hydrochloric acid to the obtained extract, adjust the pH value to 2, let it stand for 24 hours, filter with suction, add 4 times the amount of methanol to the obtained crude extract to dissolve completely, Add 8 times the volume of the crude extract to 90°C distilled water and stir, cool down naturally until the rutin recrystallizes, repeat the crystallization twice, then filter and dry the obtained crystals to obtain 14.7g of high-purity rutin. 98.6%.

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Abstract

The invention discloses a novel method for extracting rutin from pagodatree flower bud, and specifically relates to a method combining an ultramicro powder technology and continuous countercurrent ultrasound. The method comprises the following steps: after adding alkali liquor into superfine-grinded pagodatree flower bud, filtering to obtain leach liquor after continuous countercurrent ultrasonic extraction; repeatedly extracting the filter residue twice; combining the filtrate; adjusting the PH and carrying out acid precipitation; standing; filtering to obtain a rutin coarse extract; adding methanol to fully dissolve the rutin coarse extract; then, adding hot distilled water to stir; naturally cooling till rutin is recrystallized and separated out; repeatedly crystallizing twice; and then filtering and drying the crystals finally obtained to obtain high purity rutin. According to the novel method provided by the invention, least solvent is used to realize the maximum solubility, so that the novel method is high in extraction rate, short in extraction time and low in extraction cost, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a new method for extracting rutin from pagoda japonica, in particular to a method combining ultrafine powder technology with continuous countercurrent ultrasonic extraction, and belongs to the technical field of phytochemistry. Background technique [0002] Rutin, also known as rutin and vitamin P, is the main active ingredient in the flowers and buds of the leguminous plant Sophora japonica (the content of rutin in Sophora japonica is as high as 8%~28%), with a slightly bitter taste and a melting point of 177~178℃ , Slightly soluble in water, soluble in hot water and ethanol. It is perishable when exposed to light and needs to be stored in a cool place. At present, it is mainly used to treat the fragility and osmotic hemorrhage of capillaries, reduce human blood lipid and cholesterol, and treat hypertension, cardiovascular disease, stomach disease, skin disease, diabetes and other diseases. In addition, it has antibacterial ...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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