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Novel multifunctional nano diagnosis and treatment agent based on tumor multi-mode co-therapy and preparation method thereof

A synergistic, dual-mode imaging technology, applied in the field of nanobiomedicine, can solve the problems of single imaging and treatment mode, insensitivity, poor diagnosis and treatment effect, and achieve high imaging sensitivity and spatial resolution, important research significance, and low cost low effect

Inactive Publication Date: 2013-12-11
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Facing the problems existing in the existing technology, based on the current severe cancer situation and the future trend of nano-biomedical development, in the present invention, we have designed a hollow mesoporous cavity based on gadolinium-doped up-conversion fluorescent nanoparticles with a size below 80nm. The silicon oxide structure aims to provide a new multifunctional nano-therapeutic agent capable of efficient diagnosis and in situ treatment of malignant tumors, so as to overcome the single imaging and treatment mode, poor diagnosis and treatment effect, and lack of radiotherapy in the field of cancer diagnosis and treatment. Insensitivity to oxygen tumors and other shortcomings, realize the efficient diagnosis of magnetic resonance / up-conversion fluorescence dual-mode for early malignant tumors and the coordinated treatment of in situ photodynamic therapy / chemotherapy / radiotherapy

Method used

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  • Novel multifunctional nano diagnosis and treatment agent based on tumor multi-mode co-therapy and preparation method thereof
  • Novel multifunctional nano diagnosis and treatment agent based on tumor multi-mode co-therapy and preparation method thereof
  • Novel multifunctional nano diagnosis and treatment agent based on tumor multi-mode co-therapy and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Preparation of Gadolinium-doped Upconversion Fluorescent Nanoparticles Gd-UCNPs

[0052] a. First, prepare up-conversion fluorescent nanoparticles NaYF by high temperature pyrolysis 4 :Yb / Er / Tm

[0053] 1. Preparation of rare earth chlorides. Weigh 1.58mmol (479.3088mg) YCl respectively 3 -H 2 O, 0.36 mmol (139.4964 mg) YbCl 3 -6H 2 O, 0.04mmol (15.2684mg) ErCl 3 -6H 2 O, 0.02 mmol (5.5087 mg) TmCl 3 . These powders were placed in the same sample bottle, then dissolved with 4mL deionized water, and transferred to a 100mL three-necked flask;

[0054] 2. Add 15mL of oleic acid and 30mL of octadecene into the three-necked bottle, and stir at room temperature for 1 hour; then start flowing argon for 5 minutes to remove the air in the bottle, and the system starts a slow water removal process; first raise the temperature to 80 degrees, and keep 1 hour or longer (remove free water); then rise to 120 degrees and keep for 1 hour; then rise to about 156 degrees and kee...

Embodiment 2

[0068] Gd-UCNPsSiO coated with solid silica on the outer surface of Gd-UCNPs 2

[0069] The inverse microemulsion method was used to realize the encapsulation of solid silicon oxide: 1mL NP-5 was added to 20mL cyclohexane, and magnetically stirred for 40min; The color is transparent, sealed and stirred for 3h. 0.14mL of 30% ammonia water was added dropwise to the mixture, sealed and stirred for 2h. Dissolve 0.2mL TEOS in 0.8ml cyclohexane and introduce it into the system at a rate of 1mL / h; after 36h, add methanol to destroy the inverse microemulsion system, stir for 30min, collect by centrifugation, wash with ethanol and ultrasonically disperse the whole process, repeat three times , and finally dispersed in 5 mL of deionized water.

Embodiment 3

[0071] Gd-UCNPsSiO 2 Coated Gd-UCNPsSiO on the outer surface of mesoporous silica 2 mSiO 2 2g CTAC and 0.02g TEA were dissolved in 20mL deionized water and stirred at room temperature for 1.5h; then 10mL Gd-UCNPsSiO 2 deionized aqueous solution, continue to stir for 1.5h; then transfer the entire reaction system to an 80°C water bath, add 300μL TEOS dropwise, take it out immediately after reacting for 1h; then wash it repeatedly with ethanol for 3 times, and then dissolve it in mass Fraction of 1wt% NaCl methanol solution, stirred at room temperature for 3h, the whole process lasted 3 times until CTAC was completely removed; finally, the product Gd-UCNPsSiO 2 mSiO 2 Disperse into 10 mL of deionized water.

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Abstract

The invention relates to a novel multifunctional nano diagnosis and treatment agent based on tumor multi-mode co-therapy and a preparation method thereof. The multifunctional nano diagnosis and treatment agent comprises a silicon dioxide casing provided with a cavity and mesoporous channels, gadolinium-mixed upconversion fluorescence nano particles adopting core-shell structures and located in the cavity of the silicon dioxide casing, chemotherapy drug, which is loaded between the silicon dioxide casing and the gadolinium-mixed upconversion fluorescence nano particles, and has sensibilization effect on radiation treatment, and photosensitizer, which is loaded in the mesoporous channels in the silicon dioxide casing.

Description

technical field [0001] The invention relates to a novel multifunctional nano-diagnosis and treatment agent based on synergistic treatment and its preparation and application, belonging to the field of nano-biomedicine. Specifically, this nano-diagnostic agent is a core / shell cavity mesoporous silicon oxide structure with a size below 80nm. Its core gadolinium-doped up-conversion fluorescent particles can realize magnetic resonance / up-conversion luminescence dual-mode imaging diagnosis for malignant tumors; the cavity and mesoporous channels can respectively carry photosensitizers (such as hematoporphyrin) with radiosensitization effects Combined with anticancer drugs (such as docetaxel) that have radiosensitization effects, so as to realize the synergistic and efficient treatment of three modes of photodynamic therapy / chemotherapy / radiotherapy, it is expected to achieve the best therapeutic effect. Background technique [0002] At present, one treatment method is commonly u...

Claims

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Application Information

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IPC IPC(8): A61K49/06A61K49/00A61K41/00A61K47/04A61K31/337A61K33/24A61P35/00
Inventor 范文培步文博施剑林
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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