Application of epigallocatechin gallate to preparation of drugs for promoting epidermal repair and regeneration

A technology of gallate and gallic acid, which can be used in drug combinations, skin diseases, pharmaceutical formulations, etc., and can solve problems that do not involve epigallocatechin

Inactive Publication Date: 2013-12-11
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there are no reports in the prior art concerning the application of epigallocatechin gallate and its preparations in activating EGFR and promoting epidermal repair and regeneration

Method used

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  • Application of epigallocatechin gallate to preparation of drugs for promoting epidermal repair and regeneration
  • Application of epigallocatechin gallate to preparation of drugs for promoting epidermal repair and regeneration
  • Application of epigallocatechin gallate to preparation of drugs for promoting epidermal repair and regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The effect of epigallocatechin gallate EGCG on the epidermal growth factor receptor EGFR on the surface of A431 cell membrane:

[0023] A431 is a human epidermal carcinoma cell line capable of subculture. Cultured in RPMI1640 medium containing 10% fetal bovine serum (Hyclone) and 1% penicillin and streptomycin. Cultivate to the best state (few dead cells, it can be clearly seen that the cells are obviously spindle-shaped), divide into 40 plates, and inoculate 5 million cells per plate.

[0024] After 24 hours, the subcultured A431 cells were sucked out of the culture medium, washed with PBS, starved with serum-free medium (for at least 16 hours), the culture medium was sucked away, washed with PBS, and cultured with pH 6.5 acid Base treatment 30min, each treatment 10 plates of cells, four treatments, the control added an equal volume of serum-free medium pH6. -200ug / ml epigallocatechin gallate EGCG, 20ng / ml epidermal growth factor EGF, 0-200ug / ml+20ng / ml EGCG+EGF, aft...

Embodiment 1

[0028] From commercially available epigallocatechin gallate (EGCG), routinely add water for injection, fine filter, potting and sterilization to make injection.

Embodiment 2

[0030] Commercially available epigallocatechin gallate (EGCG), dissolved in sterile water for injection, stirred to dissolve, filtered with a sterile suction filter funnel, then sterile fine filtered, divided into 2 ampoules In the process, after low-temperature freeze-drying, the powder injection was aseptically melt-sealed.

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PUM

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Abstract

The invention provides an effect of activating serine, threonine and tyrosine phosphorylation of an epidermal growth factor receptor (EGFR) by epigallocatechin gallate (EGCG), and the effect is similar to an epidermal growth factor (EGF), that is, the EGCG can serve as the EGF of a plant origin to be applied to promotion of epidermal repair and regeneration. The invention provides application of the EGCG to preparation of drugs and cosmetics for promoting epidermal repair and regeneration. The invention further provides the EGCG serving as the epidermal growth factor, an epidermal growth factor receptor activator and an epidermal growth factor receptor signal channel promoter.

Description

technical field [0001] The invention belongs to the fields of natural medicinal chemistry and drug development and application, and in particular relates to the application of epigallocatechin gallate and its preparation in activating EGFR and promoting epidermis repair and regeneration. technical background [0002] Tea is one of the most popular beverages in the world. It has many functions such as anti-oxidation, anti-aging, anti-bacterial and anti-virus, anti-tumor, and reducing the incidence of certain diseases. The composition of tea is very complex. So far, more than 500 compounds have been isolated and identified in tea, including more than 450 organic compounds and dozens of inorganic nutrients. In the water extract of tea leaves, catechins account for 25-40%, and epigallocatechin gallate (EGCG) accounts for 60% of catechins. EGCG is considered to be the main active ingredient in tea leaves and green tea. The research on the efficacy of tea at home and abroad also...

Claims

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Application Information

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IPC IPC(8): A61K31/353A61P17/00A61K8/49A61Q19/00
Inventor 盛军郝淑美黄艳苹刘易凡王宣军
Owner YUNNAN AGRICULTURAL UNIVERSITY
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