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New use of tooth-related stem cells

A periodontal ligament stem cell and stem cell technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of high cost, inability to be routinely used, and high disease recurrence rate

Inactive Publication Date: 2013-12-11
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hematopoietic stem cell transplantation can make some patients achieve disease control, but it is expensive, has many complications, and the recurrence rate of the disease is as high as 40%-50%, so it cannot be used routinely; and although some patients may achieve long-term remission after transplantation, all Autoimmune diseases cannot be completely cured, and some patients have recurrence problems

Method used

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  • New use of tooth-related stem cells
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  • New use of tooth-related stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Example 1: Inhibitory effect of PDLSCs on T lymphocyte proliferation

[0091] Materials and methods

[0092] 1), human PDLSCs

[0093] The normal third molars of 18-28-year-old healthy patients extracted from the Department of Oral and Maxillofacial Surgery, Beijing Stomatological Hospital Affiliated to Capital Medical University were selected, and PDLSCs were isolated and cultured according to the methods reported in the previous literature (3). The use of human tissue was approved by the Ethics Committee of Capital Medical University. In order to apply cryopreserved periodontal ligament stem cells (cPDLSCs) in the next experiment, some freshly isolated periodontal ligament stem cells (fPDLSCs) were frozen in liquid nitrogen for 3 months. After 3 months, the frozen cells were quickly thawed in a 37°C water bath, inoculated, and routinely cultured. In this study, the cells used were all at passage 2-4. In the same experiment, fPDLSCs and cPDLSCs of the same ge...

Embodiment 2

[0114] Example 2: PDLSCs-mediated periodontal bone defect repair

[0115] 1), materials and methods

[0116] Wuzhishan miniature pigs and Guizhou fragrant pigs (6-8 months old, weighing 30-40 kg) were provided by the Experimental Animal Center of China Agricultural University. This experiment was approved by the Ethics Committee of Capital Medical University. The isolation and culture methods of minipig canine PDLSCs are the same as human PDLSCs. According to the method reported in the literature (13), 12 female Wuzhishan minipigs with periodontitis bone defect models were prepared, and a total of 24 mandibular first permanent molars with periodontitis bone defects were prepared. The 24 defects were randomly divided into the following 4 groups: [1] Blank control group, without any treatment; [2] Simple material group, flap flap, curettage, transplantation of HA / TCP scaffold material (provided by Wuhan University of Technology), gelatin Sponge covered the defect and sutu...

Embodiment 3

[0142] Example 3: Biological root regeneration mediated by tooth-associated mesenchymal stem cells

[0143] 1) Materials and methods

[0144] 1-1) Isolation and cultivation of seed cells

[0145] Stem cells from the apical papilla: Miniature pig canines were aseptically extracted under anesthesia, and the apical part of the apical papilla was excised, washed repeatedly with D-Hank's solution, cut into small pieces, and placed in a medium containing type Ⅰ collagenase (3g / L) and Dispase (4g / L) The digestion solution was digested at 37°C for 1 h, the cells were collected through a 70 μm filter, centrifuged at 1000 r / min for 10 min, and resuspended with culture medium to form a single cell suspension. Press 0.01~1×10 5 Apical papilla cells and periodontal ligament cells were inoculated in 6-well plates, respectively, in α-MEM medium (containing 15% fetal bovine serum, 2mmol / L glutamine, 100U / ml penicillin, 100μg / ml Streptomycin) 37℃, 5%CO 2 For culture, change the med...

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Abstract

New uses of tooth related stem cells in preparing products for preventing or treating tooth related diseases, immune diseases, autoimmune diseases, diseases related to abnormal activation or enhancement of T lymphocytes, lupus erythematosus or systemic lupus erythematosus, or products for repairing the tooth related tissues are provided. Compositions containing the tooth related stem cells and methods for preventing or treating diseases by using the tooth related stem cells are also provided.

Description

[0001] This application is a divisional application of the invention application with the application number 201080005266.5 and the title of the invention "New Application of Teeth Related Stem Cells". field of invention [0002] The present invention relates to a new application of tooth-related stem cells. Specifically, the present invention relates to the use of tooth-related stem cells in the preparation of products for the prevention or treatment of diseases or conditions related to teeth, or the use of products for the formation or repair of tooth-related tissues; Stem cells are used in the prevention or treatment of immune diseases or conditions, autoimmune diseases or conditions, diseases or conditions related to abnormal activation of T lymphocytes, diseases or conditions related to abnormal increase of T lymphocytes, lupus erythematosus, or Use in a product for systemic lupus erythematosus; also to a method of treatment according to the above use; also to a compositi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/00A61K35/32C12N5/0775A61P1/02A61P37/02
CPCC12N5/0664A61P1/02A61P37/00A61P37/02
Inventor 王松灵施松涛丁刚
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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