Breeding method for third-degree seeds of grifola shoestring fungus

A technology of dense ring bacteria and three-level species, applied in botany equipment and methods, horticulture, application, etc., can solve the problems of high cost of cultivation, slow growth of bacteria, etc., achieve wide source of raw materials, high production efficiency, and avoid harmful The effect of the influence of substances and bacteria

Inactive Publication Date: 2013-12-11
陕西庆瑞丰食药用菌科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a breeding method for the three-level species of Polyporus coliformis, which solves the problems of slow growth and high cultivation cost of the prior art when cultivating the three-level species of Polyporus coliformis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The breeding method of the tertiary species of Polyporus coliformis comprises the steps:

[0027] The first step is to prepare materials

[0028] Harvesting diameter is the linden branch of 5mm, and branch is cut into the segment of 28mm;

[0029] Step two, soak

[0030] Put the branch section obtained in the first step into the container, add clear water until the water has not passed the branch section, and soak for 23 hours;

[0031] The third step, bottling

[0032] Take out the small pieces of branches that have been treated in the second step, drain the water, and put them into the strain bottle, the filling amount is 95% of the capacity of the strain bottle, add water to the mouth of the strain bottle, and then tighten the strain bottle cover;

[0033] The fourth step, sterilization

[0034] Put the strain bottle obtained in the third step into the sterilization box for sterilization. The temperature of the sterilization box is set to 105°C and the pressure ...

Embodiment 2

[0043] The breeding method of the tertiary species of Polyporus coliformis comprises the steps:

[0044] The first step is to prepare materials

[0045] Harvesting diameter is the maple branch of 10mm, branch is cut into the segment of 32mm;

[0046] Step two, soak

[0047] Put the small branch of the branch obtained in the first step into the container, add clear water until the water has not passed the small branch of the branch, and soak for 25 hours;

[0048] The third step, bottling

[0049] Take out the small pieces of branches that have been treated in the second step, drain the water, and put them into the strain bottle, the filling amount is 98% of the capacity of the strain bottle, add water to the mouth of the strain bottle, and then tighten the strain bottle cover;

[0050] The fourth step, sterilization

[0051] Put the strain bottle obtained in the third step into the sterilization box for sterilization. The temperature of the sterilization box is set to 115...

Embodiment 3

[0060] The breeding method of the tertiary species of Polyporus coliformis comprises the steps:

[0061] The first step is to prepare materials

[0062] Harvesting diameter is the hazel branch of 8mm, branch is cut into the segment of 30mm;

[0063] Step two, soak

[0064] Put the small branches of the branches obtained in the first step into the container, add clear water until the water has not passed the small branches of the branches, and soak for 24 hours;

[0065] The third step, bottling

[0066] Take out the small pieces of branches that have been treated in the second step, drain the water, and put them into the strain bottle, the filling amount is 96% of the capacity of the strain bottle, add water to the mouth of the strain bottle, and then tighten the strain bottle cover;

[0067] The fourth step, sterilization

[0068] Put the strain bottle obtained in the third step into the sterilization box for sterilization. The temperature of the sterilization box is set...

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Abstract

The invention discloses a breeding method for third-degree seeds of grifola shoestring fungus. The breeding method comprises seven steps of preparation, soaking, bottling, sterilization, cooling, inoculation and cultivation. The problems that strains are low in growth speed and the cultivation cost is high when the third-degree seeds of the grifola shoestring fungus are bred in the prior art is solved by the breeding method disclosed by the invention. According to the breeding method for the third-degree seeds of the grifola shoestring fungus disclosed by the invention, broad leaf branches are directly cut to serve as base materials, and thus, raw materials are wide in source, and the cultivation cost of the breeding method disclosed by the invention is lower than that of a traditional method for breeding the third-degree seeds of the grifola shoestring fungus by adopting bacteria sticks. Moreover, the culture base materials adopted by the breeding method disclosed by the invention are convenient to fill, and the production efficiency is high. According to the breeding method disclosed by the invention, influences of harmful substances and infectious microbes can be effectively avoided by a culture medium, and the survival rate of the strains can reach 99.9 percent.

Description

technical field [0001] The invention belongs to the technical field of microbial breeding, and in particular relates to a breeding method for tertiary species of Clostridium polyporus. Background technique [0002] Polyporus is a commonly used fungal medicinal material in my country. It is also known as wild boar manure, ground black peach, and powdery polyporus, etc. It has a medicinal history of more than 2,000 years. It is a commonly used Chinese medicinal material. Diuretic treatment of edema effect. In recent years, studies have found that Polyporus polyporus polysaccharide (glucan) has anti-cancer effects and is one of the main raw materials for tumor treatment drugs. Amycetes is a genus of Basidiomycetes Agaricaceae fungi, which are widely distributed in Yunnan, Hebei, Shanxi, Heilongjiang, Jilin, Zhejiang, Fujian, Guangxi, Shaanxi, Gansu, Xinjiang, Sichuan, Tibet, etc. It is found everywhere, mainly in hardwood forests or mixed conifer forests. "Relationship betwee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 杨新庆
Owner 陕西庆瑞丰食药用菌科技有限公司
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