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Reagents and test methods with the test group before a transfusion

A combination test and reagent group technology, which is applied to the reagent group and the test field of the combination test before blood transfusion, can solve the problems of blood transfusion incompatibility, poor detection sensitivity, etc., reduce the amount of use, standardize the inspection process, reduce The effect of testing costs

Inactive Publication Date: 2013-12-04
林妈利 +1
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Problems solved by technology

[0007] In addition, the key to the accuracy of the traditional manual polybrene method is that the ratio of plasma (or serum) to hemocytes is greater than 50 times, and the detection sensitivity is not good if it is lower than 50 times; generally speaking, the ratio of the traditional manual polybrene test tube method About 66.67 times (ie 100μL: 1.5μL)
However, as mentioned above, in addition to the two major blood types of "ABO blood group system" and "Rh blood group system" that must be confirmed, there are other blood group systems that may also cause blood transfusion incompatibility reactions. Therefore, if further For more detailed antibody screening or identification, it is necessary to draw more blood samples from the patient, which is undoubtedly worse for an ischemic patient

Method used

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  • Reagents and test methods with the test group before a transfusion
  • Reagents and test methods with the test group before a transfusion
  • Reagents and test methods with the test group before a transfusion

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Embodiment 1

[0043] The composition ratio of the reagent group proposed according to the present invention is as follows:

[0044] 1. The blood cell cleaning solution: composed of 20 solution and sodium chloride solution with a concentration of 0.9% are uniformly mixed at a liquid volume ratio of 1:10000.

[0045] 2. The low-ion medium solution: 0.05 grams of ETDA sodium salt (Na 2 EDTA·2H 2 O) Dissolved in 90mL of Dextrose solution with a concentration of 5%, and then uniformly mixed with dextran with a concentration of 10% at a liquid volume ratio of 9:1.

[0046] 3. The polybrene solution: first prepare polybrene powder and sodium chloride solution to prepare a polybrene solution with a concentration of 0.4%, and then mix it with a polyethylene glycol solution with a concentration of 20% at a liquid volume ratio of 1:1.

[0047] 4. The resuspension solution: it is composed of trisodium citrate solution with a molar concentration of 0.4M and a Dextrose solution with a concentration o...

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Abstract

The present invention provides a reagent set with the test method and test before a blood transfusion, is a method to improve the traditional manual polybrene method and the detection sensitivity of the detection reagent set, the cleaning liquid reagent set comprising blood, a solution of low ionic medium, polybrene solution and resuspension solution. The method and reagent set of the present invention made without clinical significance of antibodies has a lower detection sensitivity, but the clinical significance of the antibody has a high detection sensitivity, thus reducing the workload for the subsequent identification of the antibody, reducing testing costs, giving patients more secure rapid transfusion quality.

Description

technical field [0001] The invention relates to a reagent set and a test method for a pre-blood transfusion coordination test, in particular a reagent set and a test method capable of improving the detection sensitivity of the traditional manual polybrene method. Background technique [0002] Blood type is a method of classifying blood and is a genetic feature. Clinically, blood type refers to the difference in the structure of red blood cell surface antigens. In a broad sense, blood types include antigen systems with different blood components such as red blood cells, white blood cells, platelets, and plasma. The earliest blood group system recognized by humans is the ABO blood group system. For example, red blood cells containing A antigens are called type A; this was discovered in 1900 by Karl Landsteiner of the Institute of Pathology, University of Vienna, Austria. The blood plasma of healthy people has agglutinating effect on the red blood cells of different human indi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/50
Inventor 林妈利詹咏絮
Owner 林妈利
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