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Sphingomonas paucimobilis strain and application thereof

A technology of monocysts and oligokinetic sheaths, applied in the field of sphingomonas oligokinetic strains, which can solve the problems of unreported endophytic bacteria strains

Inactive Publication Date: 2013-12-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on microbial strains producing gellan gum, but there are almost no reports on the isolation of endophytic bacterial strains capable of producing gellan gum from plant tissues

Method used

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  • Sphingomonas paucimobilis strain and application thereof
  • Sphingomonas paucimobilis strain and application thereof
  • Sphingomonas paucimobilis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Isolation, purification, identification and preservation of the bacterial strain of embodiment 1

[0033] 1. Isolation and purification of strains

[0034] An endophyte strain was isolated and screened from the seeds of wild Microporia spp. It is specifically: using the microporous grass seeds picked from the Qinghai plateau as the isolated raw material, the raw material is cleaned, ground to a size of 60 mesh, and aseptically treated (alcohol treatment for 1 minute, then 3.5% sodium hypochlorite disinfection for 5 minutes, and then Disinfection alcohol treatment for 1 minute, sterile water washing twice), and finally separate and inoculate, repeat at least 3 pieces for each petri dish, and place in an incubator at 25-28°C for cultivation. When colonies visible to the naked eye grow in the culture medium, they are inoculated onto LB broth plates by inoculation method for natural purification and separation, and the target strains are preserved.

[0035] 2. Identificat...

Embodiment 2

[0045] Example 2 Preparation of Gellan Gum by Fermentation of Sphingomonas paucimobilis QHZJUJW

[0046] (1) Activation of the strain: use an inoculation loop to inoculate Sphingomonas paucimobilis QHZJUJW by streaking on the activation medium, and culture it in a constant temperature incubator for 24-72 hours, and set the culture temperature to 28±2°C; Wherein, the activation medium is YP or LB medium, and YPD includes the following raw materials (in weight percentage): Yeast Extract (yeast extract powder) 1%, Peptone (peptone) 2%, Dextrose (glucose) (glucose) 2%, Agar powder 2%, prepared with distilled water, pH7.2; LB includes the following raw materials (by weight percentage): Yeast Extract (yeast extract powder) 0.5%, Tryptone (tryptone) 1%, NaCl (sodium chloride) 1%, Agar powder 2%, prepared with distilled water, pH7.2;

[0047] (2) Preparation of seed liquid: Inoculate the single colony of Sphingomonas paucimobilis QHZJUJW obtained in step (1) into the seed culture liq...

Embodiment 3

[0050] Example 3 Preparation of Gellan Gum by Fermentation of Sphingomonas paucimobilis QHZJUJW

[0051] (1) Activation of the strain: use an inoculation loop to inoculate Sphingomonas paucimobilis QHZJUJW by streaking on the activation medium, and culture it in a constant temperature incubator for 24-72 hours, and set the culture temperature to 28±2°C; Wherein, the activation medium is a YPD medium, including the following raw materials (in weight percentage): Yeast Extract (yeast extract powder) 1%, Peptone (peptone) 2%, Dextrose (glucose) (glucose) 2%, agar powder 2%. %, prepared with distilled water, pH7.2.

[0052] (2) Preparation of seed liquid: Inoculate the single colony of Sphingomonas paucimobilis QHZJUJW obtained in step (1) into the seed culture liquid of 50 ml, and place it in a constant temperature shaker for 24 h, Temperature 30±1°C, rotating speed 200r / min; wherein, the seed culture solution includes the following raw materials (by weight): 0.5% sucrose, 0.1% ...

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Abstract

The invention discloses a sphingomonas paucimobilis strain and an application thereof. The sphingomonas paucimobilis strain is named as sphingomonas paucimobilis QHZJUJW, with the preservation number: CGMCC No.7783. The stain is endophyte derived and screened from seeds of wild microporous grass in Qinghai-Tibet Plateau, can be used for industrialized production of gellan gum and provides a new way for the industrialized production of the endophyte and gellan gum.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a strain of Sphingomonas paucimobilis and its application. Background technique [0002] Microbial polysaccharides are generally non-toxic, harmless, degradable, and non-polluting to the environment. Compared with plant polysaccharides and animal polysaccharides, they have the advantages of a short growth cycle and are not affected by seasons, climates, and geographical conditions. Due to its wide variety, different structures, and unique and diverse physical and chemical properties, it is used in food, medicine, daily light chemicals, petroleum and other industries. At present, the research on microbial polysaccharides has attracted more and more attention, and the research on bacterial polysaccharides has also been expanding. In order to improve the production and fermentation ability of microbial polysaccharides, more and more microbial polysaccharides such as ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 陈启和张俊董亚晨焦迎春范琳琳
Owner ZHEJIANG UNIV
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