Triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum/ chlamydia trachomatis
A detection kit, the technology of Ureaplasma urealyticum, applied in the biological field, can solve the problems of low sensitivity and specificity, and achieve the effects of high sensitivity, pollution prevention and strong repeatability
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[0051] Example 1 A nucleic acid detection kit for rapid detection of Neisseria gonorrhoeae / Ureaplasma urealyticum / Chlamydia trachomatis.
[0052] The above kit includes RT-PCR reaction solution, enzyme mixture, Neisseria gonorrhoeae (NG) / Ureaplasma urealyticum (UU) / Chlamydia trachomatis (CT) triple reaction solution, positive control and negative control;
[0053] Wherein, the PCR reaction solution includes 10× buffer, 25mM MgCl 2 , 10mM dUTP and 10mM dNTPs;
[0054] The triple reaction liquid of gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis comprises the following components:
[0055] Component (1): composed of a pair of primers for detecting Neisseria gonorrhoeae and a probe for detecting Neisseria gonorrhoeae; wherein, the base sequences of the two primers are respectively shown in SEQ ID No.1 and SEQ ID No.2; The base sequence of the needle is shown in SEQ ID No.3, the 5' end of the probe is marked with a fluorescent reporter group, and the 3' end is marked wi...
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