Preparation method of rice protein oligopeptides

Abstract

The invention relates to a preparation method of rice protein oligopeptides, which comprises the steps of raw material pretreatment, pulverization, size mixing, washing, size mixing, reaction, concentrating, dehydration and the like. The method provided by the invention enhances the enzyme reaction substrate, and maximally displays the efficiency of the enzyme preparation, thereby reducing the consumption of the enzyme preparation and avoiding the separation and purification of the soluble oligopeptide product from other soluble impurities. The protein content of the product on dry basis is greater than 90%, the ash content is less than 2.7%, the fat content is less than 0.1%, the protein extraction rate is greater than 70%, and the oligopeptides with the molecular weight of 200-700 account for more than 91.5% of the total protein. The product is completely soluble in water, can be widely used in the fields of health food, nutritional food, sports food and the like, and greatly enhances the added value of rice deep processing.

Description

【Technical field】 [0001] The invention belongs to the technical field of rice deep processing. More specifically, the present invention relates to a preparation method of rice protein peptide. 【Background technique】 [0002] Rice protein has excellent nutritional quality, and its nutritional value is much higher than that of corn, wheat and other plant proteins, and comparable to high-quality animal proteins such as eggs and milk. The amino acid composition of rice protein is very close to the ideal model recommended by WHO / FAO. The methionine content of rice protein is as high as 2.2%, which is unmatched by other plant proteins and even many animal proteins. The content of arginine in rice protein is also high, and rice protein is hypoallergenic. It is currently the only grain that can be exempted from allergy experiments, and it has unique advantages when used in infant food. [0003] The protein content in rice is about 6-8%. From an economical point of view, the prote...

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Problems solved by technology

[0008] However, there are very few naturally occurring peptides at present. The production of peptides is mainly obtained by proteolysis. The methods of proteolysis mainly include acid method, alkali method and enzymatic method. Complicated, difficult to control the degree of hydrolysis, serious environmental pollution and other disadvantages, and in the case of high alkali or high acid, protein reacts with impurities such as sugar and oil to produce brown substances, and even produce harmful substances, which will affect the final peptide product quality

The reaction of enzymatic hydrolysis of protein is relatively mild, and no adverse side reactions occur, but the singleness of enzyme action makes it difficult to achieve the ideal hydrolysis effect by one enzyme, and the protein extraction rate is low, the molecular weight distribution of the obtained product is wide, and the proportion of active peptides Shortcomings such as low ratio, such as CN200610010960.1 proposes a method for the production of rice starch and rice protein polypeptide powder by a compound enzyme method, wherein four proteases, alkaline protease, neutral protease, acid protease and flavor protease, are used, and after hydrolysis reaction The obtained supernatant is processed to obtain rice protein polypeptide powder

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