PHKG1 (phosphorylase kinase, gamma 1) gene and application thereof in genetic improvement for breeding pig meat quality traits

Abstract

The invention discloses a nucleotide sequence or an amino acid sequence of a PHKG1 (phosphorylase kinase, gamma 1) gene located on pig chromosome 3, wherein a mutation site of the PHKG1 gene is the C-A mutual mutation of a mononucleotide at the 8118th site of a sequence shown in SEQ ID NO: 1; when C is mutated to A, a normal transcript generated by the PHKG1 gene in a transcription process deletes abnormal transcripts of 32 bp fragments, and the deleted 32 bp fragments are located at the 8123rd-8154th base of 5' end of the sequence shown in SEQ ID NO: 1; on the basis, disadvantageous genotype individuals are eliminated by selecting advantageous genotype individual reserve breeding pigs, so that population meat quality traits can be remarkably improved, especially, muscle glycogen content can be reduced, drip loss and processing loss can be reduced, and pH value and intramuscular fat content can be increased, thus the pig meat quality is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a PHKG1 gene that affects pork quality traits such as hydraulic power (dripping loss), pH, and muscle glycogen glycolysis potential, and its application in genetic improvement of breeding pigs. Background technique [0002] Improving pork quality has become a common goal of the pig industry all over the world. At present, one of the main problems of pork quality is the low hydraulic system. Due to the low hydraulic system, the output of pork decreases during processing and sales, and the eating quality decreases, which seriously affects the commercial value of pork. [0003] Past studies have shown that muscle glycogenolysis and pH drop are the main factors affecting the hydraulic power of pork. After the pig is slaughtered, muscle glycogen will be fermented to produce lactic acid, resulting in a drop in pH; when the pH drops to near the isoelectric point of myofibrillar...

AI Technical Summary

Problems solved by technology

[0006] Although in PRKAG3 (aka RN - ) has made a breakthrough in the identification of causal genes, but people's understanding of the genetic factors affecting GP and system hydraulics is still very limited

For example, there is no polymorphism in the R200Q locus in Duroc, Landrace, Large White and other Western commercial pig breeds, but Costa et al. , the GP of about 10% of the individuals exceeds 180 μmol / g, and they also show low pH and low hydraulic power, which indicates that there are other major genes affecting GP besides PRKAG3

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