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Liposome preparation method and method for preparing liposome adjuvant

A lipid and system technology, applied in the field of biomedicine, to achieve good protein loading capacity and immune response level, easy to prepare in large quantities, and simple operation

Active Publication Date: 2013-11-27
SHANGHAI ZERUN BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the field of using liposomes for vaccines in China.

Method used

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  • Liposome preparation method and method for preparing liposome adjuvant
  • Liposome preparation method and method for preparing liposome adjuvant
  • Liposome preparation method and method for preparing liposome adjuvant

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Embodiment Construction

[0026] Weigh the hydrogenated soybean phospholipids HSPC, cholesterol CHOL, 1,2-dioleoyloxytrimethyl ammonium chloride DOTAP-Cl, vitamin VE, according to the mass fraction of 70~90:0~30:10~20:0.5 To prepare a mixture, 2) dissolve the mixture described in step 1 in a chloroform / methanol solvent to obtain a lipid organic solution for later use. The chloroform / methanol solvent is composed of chloroform, methanol and ultracapsules in a volume ratio of 24:72:4-5.1 Pure water composition; 3) Weigh the water-soluble powdery solid support and place it in a 250ml round-bottomed flask, then add the organic solution of the above lipids, stir evenly, and place on the rotary evaporator on an ice / water bath to rotate at a constant temperature The organic solvent is evaporated, wherein the mass of the solid support is 5 times the mass of the mixture, including one or more of lactose, sucrose, mannitol, and sodium chloride; 4) Take off the round bottom flask and take out the solid The sample, ...

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Abstract

The invention relates to a liposome preparation method and an application of liposome as a vaccine immunity adjuvant. The liposome preparation method provided by the invention comprises the steps of 1) weighing hydrogenated soy phosphatidylcholine HSPC, cholesterol CHOL, 1,2-dioleoyl-3-trimethylammonium-propane, chloride DOTAP-Cl or 1,2-distearoyl-sn-glycero-3-phosphatidylglycerol sodium salt DSPG-Na, and vitamin VE, and mixing in percentage of (70-90): (0-30): (10-20): 0.5 by mass to obtain a mixture; 2) dissolving the mixture obtained from the step 1 in a chloroform / methanol solvent to obtain a solution, wherein the chloroform / methanol solvent consists of chloroform, methanol and ultra-pure water at a volume ratio of 24: 72: (4-5.1); and 3) weighing a solid support, adding the organic solution of previous lipoids, uniformly stirring, and rotatably evaporating to remove an organic solvent. The preparation method is simple in process and low in cost, and is applicable to mass cultivation of liposome and the vaccine immunity adjuvant.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing liposomes and their application as vaccine immune adjuvants. Background technique [0002] Vaccination is a very effective method to prevent and treat diseases, but some traditional vaccines can cause side effects of varying degrees. In recent years, with the continuous deepening of immunological research and the rapid development of genetic engineering technology, the research of new vaccines such as live vector vaccines, DNA vaccines and protein vaccines has made gratifying progress. These new vaccines have high purity, strong specificity, but weak immunogenicity, and the immune response induced by the body is not strong enough. Therefore, the use of safe and effective adjuvants to enhance the vaccine effect has become an urgent problem to be solved. [0003] Currently, the only adjuvant officially approved for use in human vaccines is aluminum adjuvant (the main com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/28A61K47/34
Inventor 刁美君沈琼
Owner SHANGHAI ZERUN BIOTECHNOLOGY CO LTD
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