Method for preparing malus baccata leaf alcohol extract, and antioxidation and liver protection effects thereof

A technology of vitex leaf alcohol and vitex leaf, applied to medical preparations containing active ingredients, anti-toxins, digestive system, etc., can solve problems such as increased membrane permeability, damage to liver cell membrane structure and functional integrity

Inactive Publication Date: 2013-11-20
大连杰信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The toxic metabolite CCl3 is further converted into trichloromethyl peroxy radical by P4502E1 enzyme. This covalent compound can cause the degradation of cell membrane peroxidation and increase membrane permeability, resulting in a large amount of ALT in the cytoplasm released into the blood , thereby damaging the integrity of the liver cell membrane structure and function[17]

Method used

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  • Method for preparing malus baccata leaf alcohol extract, and antioxidation and liver protection effects thereof
  • Method for preparing malus baccata leaf alcohol extract, and antioxidation and liver protection effects thereof
  • Method for preparing malus baccata leaf alcohol extract, and antioxidation and liver protection effects thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The preparation of embodiment 1 Vitexia leaf alcohol extract

[0089] Weigh 0.1kg of Vitexia leaves dried in the shade, crush them, add 2.5L of 30% ethanol, and extract by ultrasonic for 30min at 40°C, 40HZ, 250W, three times, and combine the three extracts. Concentrate under reduced pressure to an extract, and freeze-dry to obtain the alcohol extract of Vitexia leaves.

Embodiment 2

[0090] The preparation of embodiment 2 Vitexia leaf alcohol extract

[0091] (1) Weigh 0.1 kg of Vitexia leaves dried in the shade, remove impurities and pulverize.

[0092] (2) 2.5L of 70% ethanol was added, under the conditions of 40°C, 40HZ, 250W, ultrasonically extracted for 30min, extracted 3 times, centrifuged and filtered to remove the residue to obtain the filtrate.

[0093] (3) Ethanol is recovered from the filtrate below 25°C, and the remaining extract is freeze-dried to make an extract or dry powder.

[0094] (4) Subpackage to obtain the alcohol extract of Vitexia leaves.

Embodiment 3

[0095] The preparation of embodiment 3 Vitexia leaf alcohol extract

[0096] 1) Weigh 0.1 kg of Vitexia leaves after drying in the shade, remove impurities and pulverize.

[0097] 2) 2.5 L of 90% ethanol was added, and ultrasonically extracted for 30 min under the conditions of 40° C., 40 HZ, and 250 W, extracted three times, centrifugally filtered, and the residue was removed to obtain a filtrate.

[0098] 3) The ethanol is recovered from the filtrate below 25°C, and the remaining extract is freeze-dried to make an extract or dry powder.

[0099] 4) Subpackage to obtain the Alcohol Extract of Vitexia Leaf.

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Abstract

The invention provides a method for extracting and preparing a malus baccata leaf alcohol extract, and applications thereof on antioxidation and acute liver injury, the method is characterized by: weighting malus baccata leaves dried in the shade, crushing, adding 30-99% ethanol in a material and liquid ratio of 1:25-1:30, ultrasonically extracting for 30-90min in conditions of 40 DEG C, 40Hz and 250W, extracting for 1-5 times, filtrating and merging extracts, pressure-reduction condensing until to be a cream, and freeze-drying to obtain the malus baccata leaf alcohol extract. The invention further discloses the malus baccata leaf alcohol extract, which is rich in flavones and phlorizin, has antioxidation and free radical removal functions, has substantial improvement and protection effects for acute liver injury, makes effects on prevention, treatment and auxiliary treatment of hepatopathy, and particularly has a substantial effect on acute liver injury caused by peroxy like alcohol stimulation.

Description

technical field [0001] The present invention relates to the preparation of the alcohol extract of the leaves of Vitexia vitae and its protective and antioxidative effects on acute liver injury. It belongs to the field of pharmaceutical technology. Background technique [0002] Malus baccata (Linn.) Borkh.], also known as Malus baccata (Linn.) Borkh., is a perennial woody plant of Rosaceae (Rosaceae) Malus, mainly distributed in Northeast my country, North China and Southwest China, etc. It is also distributed in North Korea and Siberia, Russia [1]. The current research mainly focuses on its greening and viewing and drought resistance [2-3], and there are few reports on its chemical composition. The volatile oil components of Vitex flowers and leaves are mainly terpenoids, esters and olefins, and the main component is farnesene[4]. Polyphenols and flavonoids are the main active substances of Vitex chinensis, among which phlorizin is one of its pharmacologically active compo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P39/06A61P1/16A61K127/00
Inventor 郑毅男丁传波赵婷李永娟董岭陈大勇张传奇刘文丛李伟弓晓杰高飞飞
Owner 大连杰信生物科技有限公司
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