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Division propagation method for large-leaved gentian

A technology of ramet propagation and chinensis, which is applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of long seedling raising period, slow growth rate, low reproduction coefficient, etc. No bacterial infection effect

Active Publication Date: 2013-11-13
成都华生现代生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent CN102630569A discloses a method for in vitro rapid propagation of Gentiana miltiorrhiza tissue culture, including selection of explants, inoculation of explants, proliferation and differentiation of callus, induction of rooting, disinfection of seedling raising substrates, and plant transplantation. Although it overcomes the problems of long breeding period and low reproduction coefficient of Gentiana miltiorrhiza, it is easy to operate and can be used for industrialized rapid seedling cultivation, but tissue culture requires high equipment conditions, and its popularization and application are limited.
[0006] Chinese patent CN1247066C discloses a kind of introduction and cultivation method of Gentiana chinensis plant, the method includes seed treatment technology, artificial seedling raising technology and introduction and cultivation technology, by carrying out physical and chemical treatment to seeds, the germination rate of plant seeds reaches 70%; The method can effectively provide high-quality seedlings according to the number of seedlings and seasonal needs; through direct seeding or artificial seedling transplanting in the original place at an altitude of 1500-4300 meters, the resources of wild Gentiana chinensis plants on the plateau can be quickly restored, and effective Guarantee the medical material quality and medicinal effect of plateau wild plant effectively, although the present invention has improved the germination rate of Gentiana chinensis seed, but utilizes the seed propagation mode to cultivate Gentiana chinensis seedling raising period is longer, and growth rate is slow, does not utilize the rapid propagation of Gentiana chinensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Gentiana chinensis branch propagation method, it may further comprise the steps:

[0017] S1. Plant screening: in July every year, select a 1-year-old plant with a height of 8 cm and a root diameter of 1 cm, which has branches and grows well, as the female parent, first shake off the soil at the root, and cut off the diseased and rotten root;

[0018] S2. Plant separation: first divide the upper part of the plant root, cut the densely grown plant with a knife along the growth direction of the underground rhizome, and divide it into 2 plants. Each plant has small roots with a diameter of 0.5cm, and minimize the wound when cutting. Keep the fibrous roots, soak and disinfect the knife with potassium permanganate for 10 minutes before cutting;

[0019] S3. Sterilization treatment: After the roots are divided, dip the incision with lime powder to sterilize in time, spread the treated root seedlings in a ventilated place, and plant and breed after 1 day;

[0020]...

Embodiment 2

[0022] Example 2: Gentiana chinensis branch propagation method, it may further comprise the steps:

[0023] S1. Plant selection: From July to August every year, select 1-year-old plants with a height of 8-10 cm and a root diameter of 2 cm, with branches and good growth as the female parent, first shake off the soil at the roots, and cut off the diseased and rotten the root of

[0024] S2. Plant separation: first divide the upper part of the root of the plant, and use scissors to cut the densely growing plant along the growth direction of the underground rhizome, and divide it into 6 plants. Each plant has a small root with a diameter of 0.3cm. When cutting, minimize the wound. Keep the fibrous roots, soak and disinfect with alcohol for 10 minutes before cutting;

[0025] S3. Sterilization treatment: After the roots are divided, dip the incision with lime powder to sterilize in time, and spread the treated root seedlings in a ventilated place to dry, and they can be plant...

Embodiment 3

[0028] Example 3: Gentiana chinensis branch propagation method is characterized in that: it comprises the following steps:

[0029] S1. Plant screening: in mid-July every year, choose a 1-year-old, 9cm in height, 1.6cm in rhizome diameter, branched and well-growing plant as the female parent, first shake off the soil at the roots, and cut off the diseased and rotten roots ;

[0030] S2. Plant separation: first divide the upper part of the plant root, and use a kitchen knife to cut the densely grown plant along the growth direction of the underground rhizome, and divide it into 4 plants. Each plant has a small root with a diameter of 0.4cm. When cutting, minimize the wound. Keep the fibrous roots, soak and disinfect with alcohol for 10 minutes before cutting;

[0031] S3. Sterilization treatment: After the roots are divided, dip the incision with lime powder to sterilize in time, spread the treated root seedlings in a ventilated place, and plant and breed after 1.5 days;

...

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Abstract

The invention discloses a division propagation method for large-leaved gentian. The division propagation method for the large-leaved gentian comprises the following steps of plant screening: selecting large-leaved gentian plants with branches and good growth vigor as female parents, shrugging off root soil, and cutting off diseased and rot roots; plant separation; separating upper parts of the plant roots, and slitting the plants growing thick by using a sharp knife along the rhizome growth direction; sterilization treatment: dipping notches with lime powder to perform the sterilization treatment, and then, spreading for drying for 1-2 days in the sun in a ventilate place; seedbed arrangement: forming pot bottom-shaped holes in a ploughed furrow; buried hole planting: putting a treated plant in each hole, enabling part of rootlets to be dispersed in each hole, filling up with fine soil, and compacting and watering with root living water. By using the division propagation method for the large-leaved gentian, the division survival rate of the large-leaved gentian can reach more than 90 percent, the growth vigor of the plants is good, and the plants cannot be infected by germs. The method has the characteristics that the seedling period of the large-leaved gentian is shortened, the operation is easy and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to the technical field of Gentiana japonica cultivation. Background technique [0002] Gentiana is a perennial herb of the Gentianaceae family. It is one of the main medicines for treating rheumatic joint pain, tuberculosis hot flashes, jaundice and other diseases. It is one of the traditional Chinese medicines in my country. Gentianidine governs cold and heat pathogens, cold-dampness and wind numbness, pain in limb joints, diarrhea, and diuresis.” Gentianidine’s traditional Chinese medicine chemical components mainly include gentianine (that is, Gentianine A, Gentianine), gentianine (that is, Gentianidine B, Gentianidine ) and genitaline C, also contains volatile oil and sugars. [0003] Gentiana is an alpine medicinal plant that mostly grows in roadsides, meadows, river beaches, ditches, forests, hillside grasslands and forest margins. It is distributed in Mongolia, Russi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00
Inventor 唐清
Owner 成都华生现代生物科技有限公司
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