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Method of evaluating degree of skin stress accumulation

An evaluation method and skin technology, applied in the field of evaluation of sensitive skin, can solve problems such as unoptimized subjects, and achieve the effect of less burden

Inactive Publication Date: 2013-10-30
FUAN KERU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, test methods using highly irritating substances such as capsinoids are not preferred for subjects

Method used

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  • Method of evaluating degree of skin stress accumulation
  • Method of evaluating degree of skin stress accumulation
  • Method of evaluating degree of skin stress accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (Experiment 1) Test of skin stress accumulation degree caused by ultraviolet radiation

[0053] (1) Stress load caused by ultraviolet rays

[0054] The test was carried out using 10 healthy men as test subjects.

[0055] As the ultraviolet irradiation device, Multiple Solar Ultraviolet Simulator Model 601 (manufactured by Solar Light Co., Ltd.) was used, and a 150W xenon lamp was used as the light source. The intensity of irradiation was adjusted so that erythema inducing pigmentation appeared on the back skin of the subject, and ultraviolet (UVB) was irradiated for 170 seconds. In addition, the accumulation of stress caused by ultraviolet rays was confirmed by comparing the ultraviolet ray-irradiated area and the non-irradiated area. Skin stratum corneum samples were collected by the tape peeling method (horny layer checker: manufactured by Asahibiomed) before artificial ultraviolet irradiation and on days 5, 11, and 17 after irradiation.

[0056] (2) Extraction met...

Embodiment 2

[0070] (Experiment 2) Stress accumulation test of hypostimulation induced by sodium dodecyl sulfate (SDS)

[0071] In order to confirm whether the stimulation of the surfactant SDS, which is known to increase HSP27 in the skin, accumulates as stress, eight men and women with healthy skin were subjected to the following test.

[0072] (1) Stress load

[0073] In 2 days, wash the inner part of the left forearm with 10% SDS aqueous solution 4 times a day every 1 hour to make the skin rough (stress state). In addition, the right forearm of the same person was washed only with water as a control. In addition, after confirming that the amount of water loss in the left forearm was higher than that before the treatment, the following measurements were started.

[0074] (2) Collection of samples and determination of water loss

[0075] After rough skin treatment (day 0), water loss was measured on day 2, day 3, day 5, day 7, and day 14, while stratum corneum samples were collected b...

Embodiment 3

[0106] (Experiment 3-2) HSP27 was measured in the face, which is often subjected to external stress, and the skin of the forearm and buttocks, which were relatively unstressed, to evaluate the degree of stress accumulated in the skin.

[0107] experiment method

[0108] (1) Sample collection

[0109] Stratum corneum samples were collected from the cheeks, jaws, forearms, and buttocks of 8 healthy women by tape stripping. 4 slices were collected from 1 site.

[0110] (2) Extraction method of protein in skin stratum corneum

[0111] 400 µl of 0.5% chaps buffer was placed in a tube for homogenization, and the above-mentioned tape-peeled horny layer test piece was placed thereinto, and protein was extracted from the horny layer on the tape by homogenization. Four specimens collected from one site were extracted together with one tube to make one sample.

[0112] (3) Quantification of skin stratum corneum protein

[0113] The amount of protein contained in the horny layer extr...

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Abstract

The purpose of the present invention is to provide a novel evaluation method which, without being burdensome to a user, is capable of evaluating the degree of skin stress accumulation and further of simply and reliably evaluating whether the skin is sensitive. To this end, the degree of stress accumulation is evaluated by non-invasively sampling the stratum corneum by means of tape stripping, etc., and by assaying the expression level of HSP27 contained therein.

Description

technical field [0001] The present invention relates to an evaluation method of stress accumulation degree of human skin and an evaluation method of sensitive skin. Background technique [0002] The skin of the human body is constantly in contact with the outside world and is subject to various stimuli. A representative example of this stimulus is ultraviolet rays. Even if ultraviolet rays do not change the skin too much in appearance, in this case, it will still induce DNA breakage in skin cells or denaturation of collagen, which will cause skin aging. Ultraviolet rays often and continuously apply stress to the skin. In addition, for women, daily activities such as washing the face and applying makeup can also cause irritation to the skin, which can accumulate on the skin as potential stress. And, if the resistance to these stimuli (stress) decreases for some reason, it is recognized as sensitive skin. The degree of accumulation of external stimuli of the skin is referr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/53A61B5/00
CPCG01N2800/20G01N2800/40G01N33/6893A61B5/443A61B5/00G01N33/50G01N33/68
Inventor 石渡潮路大石奈帆子安田知永
Owner FUAN KERU
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