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Gender identification of ginkgo biloba using molecular markers

A ginkgo and gender technology, applied in the field of ginkgo gender identification using molecular markers, can solve the problems of RAPD technology instability and failure to make up for the dominant marker characteristics of SCAR markers, and achieve the effect of reducing time and labor and protecting the urban environment

Active Publication Date: 2015-09-23
KERALA FOREST RES INST
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  • Description
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Problems solved by technology

However, these studies do not rule out the instability of RAPD technology and cannot compensate for the dominant marker characteristics of SCAR markers

Method used

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  • Gender identification of ginkgo biloba using molecular markers
  • Gender identification of ginkgo biloba using molecular markers
  • Gender identification of ginkgo biloba using molecular markers

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Embodiment Construction

[0013] Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

[0014] In order to achieve the purpose of the present invention, the present invention provides a pair of oligonucleotide primers named 'SCAR-GBM'.

[0015] As the primers for ginkgo sex identification provided by the present invention, the SCAR-GBM molecular marker has a sequence developed based on the sequence of the RAPD fragment, and this is disclosed in the present invention for the first time.

[0016] For the purposes of the present invention, "primer" refers to a single-stranded oligonucleotide sequence complementary to the nucleic acid strand to be copied, which can be used as a starting point for the synthesis of primer extension products. The length and sequence of the primers must be sufficient to enable the synthesis of extension products to initiate. The specific length and sequence of a primer may depend on the conditions under which the primer i...

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Abstract

The invention relates to a molecular marker for identifying gemale plant and male plant of gingo, more particularl, the invention relates to a method for identifying gender of gingo. Compared to the usage of random primer, the method has increased stability, and compared to the dominant marker, which can provide limit information, the method is improved. According to the mehtod, the gingo male plant specificity SCAR-GBM primer and the exsisted atp1 primer of the mitochondrial DNA which is developed in the field are used at the same time for performing multiple PCR, wherein, the SCAR-GBM primer is based on the sequence development of RAPD segment which can display the heredity of gingo male plant.

Description

field of invention [0001] The present invention relates to sex identification of Ginkgo biloba (Gingko biloba) using a molecular marker, and more particularly to a molecular marker capable of distinguishing male and female plants of Ginkgo biloba and a multiplex polymerase chain reaction used in an identification method using the molecular marker (PCR) conditions. Background technique [0002] Ginkgo trees have a long history and are known as living fossils on the earth, and are widely distributed all over the world. In Korea, ginkgo is mostly used as a street tree, and for harvesting its fruit, ginkgo. Ginkgo biloba is dioecious, consisting of a male plant to provide pollen for fruiting and a female plant for fruiting. However, when ginkgo is used as a street tree, its own unpleasant smell and fruit drop in the autumn harvest season usually impairs city appearance. Meanwhile, ginkgo farmers currently encounter problems in cultivating and developing only female ginkgo pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12N15/11C12Q1/686C12Q1/6879C12Q2537/143
Inventor 洪镕杓李济完张经焕
Owner KERALA FOREST RES INST
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