Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Protein derived from chinese wildrye and related to saltresistance and encoding gene and application of protein

A coding and genetic technology, applied in the field of protein and its coding genes and applications, can solve complex problems that affect agricultural production and reduce crop yields

Inactive Publication Date: 2013-10-30
INST OF BOTANY CHINESE ACAD OF SCI
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Today 20% of the world's farmland is affected by high-salt stress, which reduces crop yields and greatly affects agricultural production
Due to the complexity of the salt tolerance of plants, which is controlled by multiple genes, it is very difficult to improve the salt tolerance of crops using traditional breeding methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein derived from chinese wildrye and related to saltresistance and encoding gene and application of protein
  • Protein derived from chinese wildrye and related to saltresistance and encoding gene and application of protein
  • Protein derived from chinese wildrye and related to saltresistance and encoding gene and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. Acquisition of the gene LcMYB7342 related to plant salt resistance and its expression specificity detection

[0066] 1. Acquisition of the gene LcMYB7342 related to plant salt resistance

[0067] 1. Plant material processing and total RNA extraction

[0068] The seedlings of Leymus chinensis (Trin.) variety Jisheng No. 1 (Jilin Province Jisheng Leymus chinensis station) were used as materials, and the total RNA was extracted after being treated with 400mM NaCl for 10 hours, and detected by 1% agarose gel electrophoresis. Such as figure 1 shown. The extracted RNA has two obvious electrophoresis bands, which are 28S RNA and 18S RNA from top to bottom, indicating that the total RNA with higher purity and integrity has been obtained.

[0069] 2. Using 454 technology to sequence the transcriptome of Leymus chinensis

[0070] First use MACS TM The mRNA Isolation Kit (Miltenyi Company) processed the RNA extracted in the above step 1 to obtain mRNA, and then rev...

Embodiment 2

[0090] Example 2, the acquisition of LcMYB7342 gene transgenic Arabidopsis plants and their stress resistance analysis

[0091] 1. Obtaining of LcMYB7342 Gene Transgenic Arabidopsis Plants

[0092] 1. Construction of recombinant expression vector p3301-121-LcMYB7342

[0093] According to the LcMYB7342 gene amplified in the above-mentioned embodiment 1, design primers, add XbaI and BamHI restriction sites, and the primer sequences are as follows:

[0094] Primer 1: 5′-CG TCTAGA ATGAAGAACGCCTCATCCTCGAGC-3' (the underlined part is the recognition sequence of Xba I, and the following sequence is the 536-559th position of sequence 2) (sequence 3 in the sequence listing)

[0095] Primer 2: 5'-C GGATCC ATAACCAGGGCCAATTCCTTTAC-3' (the underlined part is the BamH I site, and the sequence thereafter is the reverse complementary sequence of the 1020-1042 position of sequence 2) (sequence 4 in the sequence listing)

[0096] Using the artificially synthesized LcMYB7342 gene shown in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a protein derived from chinese wildrye and related to saltresistance and an encoding gene and application of the protein. The protein provided by the invention is (a) or (b), wherein (a) is a protein consisting of an amino acid sequence shown in the sequence 1 in a sequence table and (b) is a protein which is obtained by substituting and / or losing and / or adding one or more amino acid residues of the amino acid sequence shown in the sequence 1, is related to plant saltresistance and is derived from the sequence 1. The protein provided by the invention and the encoding gene of the protein can be used as candidate genes of plant saltresistance genetic engineering and used for improving the saltresistance of plants. Twenty one days after salt stress (200Mm NaCl) treatment, all LcMYB7342 gene modified Arabidopsis seedlings are normal in phenotype, but 78.6% of wild Arabidopsis seedlings have salt damage symptoms, get wilting and turn yellow. LcMYB7342 gene can be used for cultivating and identifying varieties of resistance plants which are needed in agriculture, husbandry and ecological environmental management and has a high practical application value and a bright application prospect in agriculture and husbandry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a protein derived from Leymus chinensis and related to plant salt resistance, its coding gene and application. Background technique [0002] Unfavorable natural environment, such as low temperature, high temperature, drought and salinity, will cause a series of physiological metabolic reactions in plants, manifested as reversible inhibition of metabolism and growth, and even cause irreversible damage in severe cases, resulting in the loss of the entire plant. die. Salt stress destroys the ion balance in plants, causing osmotic stress, which in turn causes a series of physiological and metabolic reactions in plants, affecting the growth and development of plants. Today 20% of the world's farmland is affected by high-salt stress, which reduces crop yields and greatly affects agricultural production. Due to the complexity of the salt tolerance of plants, which is controlled by multiple ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 程丽琴刘公社马兴勇彭献军齐冬梅陈双燕
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products