Protein derived from chinese wildrye and related to saltresistance and encoding gene and application of protein
A coding and genetic technology, applied in the field of protein and its coding genes and applications, can solve complex problems that affect agricultural production and reduce crop yields
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Embodiment 1
[0065] Example 1. Acquisition of the gene LcMYB7342 related to plant salt resistance and its expression specificity detection
[0066] 1. Acquisition of the gene LcMYB7342 related to plant salt resistance
[0067] 1. Plant material processing and total RNA extraction
[0068] The seedlings of Leymus chinensis (Trin.) variety Jisheng No. 1 (Jilin Province Jisheng Leymus chinensis station) were used as materials, and the total RNA was extracted after being treated with 400mM NaCl for 10 hours, and detected by 1% agarose gel electrophoresis. Such as figure 1 shown. The extracted RNA has two obvious electrophoresis bands, which are 28S RNA and 18S RNA from top to bottom, indicating that the total RNA with higher purity and integrity has been obtained.
[0069] 2. Using 454 technology to sequence the transcriptome of Leymus chinensis
[0070] First use MACS TM The mRNA Isolation Kit (Miltenyi Company) processed the RNA extracted in the above step 1 to obtain mRNA, and then rev...
Embodiment 2
[0090] Example 2, the acquisition of LcMYB7342 gene transgenic Arabidopsis plants and their stress resistance analysis
[0091] 1. Obtaining of LcMYB7342 Gene Transgenic Arabidopsis Plants
[0092] 1. Construction of recombinant expression vector p3301-121-LcMYB7342
[0093] According to the LcMYB7342 gene amplified in the above-mentioned embodiment 1, design primers, add XbaI and BamHI restriction sites, and the primer sequences are as follows:
[0094] Primer 1: 5′-CG TCTAGA ATGAAGAACGCCTCATCCTCGAGC-3' (the underlined part is the recognition sequence of Xba I, and the following sequence is the 536-559th position of sequence 2) (sequence 3 in the sequence listing)
[0095] Primer 2: 5'-C GGATCC ATAACCAGGGCCAATTCCTTTAC-3' (the underlined part is the BamH I site, and the sequence thereafter is the reverse complementary sequence of the 1020-1042 position of sequence 2) (sequence 4 in the sequence listing)
[0096] Using the artificially synthesized LcMYB7342 gene shown in ...
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