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Method for preparation of red elemental selenium by photosynthetic bacteria

A technology for photosynthetic bacteria and elemental selenium, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult to obtain pure red elemental selenium, difficult to separate, etc., and achieves easy construction, high purity, and growth. fast reproduction effect

Inactive Publication Date: 2013-10-23
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But find from above preparation method, collect directly after solid fermentation culture, be difficult to make red elemental selenium and culture medium, thalline separate namely that what this method makes is thalline-red elemental selenium mixture, be difficult for obtaining the very high red of purity Elemental Selenium

Method used

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  • Method for preparation of red elemental selenium by photosynthetic bacteria
  • Method for preparation of red elemental selenium by photosynthetic bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Prepare media with the following components: MgSO 4 ·7H 2 O150mg, CaCl 2 2H 2 O80mg, sodium acetate 1800mg, EDTA20mg, yeast extract powder 1200mg, K 2 HPO 4 1000mg, (NH 4 ) 2 SO 4 1500mg, FeSO 4 ·7H 2 O10mg, KH 2 PO 4 800mg, trace element solution 3ml, deionized water 1000ml, pH 7.

[0024] Wherein the trace element solution is composed of: H 3 BO 3 240mg, MnSO 4 4H 2 O200mg, Na 2 MoO 4 2H 2 O70mg, CuSO 4 7mg, ZnSO 4 ·7H 2 O24mg, deionized water 100ml.

[0025] Insert 1 / 10 of the photosynthetic bacteria into the sterilized culture medium, under light intensity of 1000lux, 30°C, and anaerobic conditions, cultivate for 5 days, repeat twice, and use as the inoculation strain for subsequent use.

[0026] The above-mentioned activated photosynthetic bacteria liquid was inoculated in the 1.0mmol / L selenite medium containing 15% of the total amount, and cultivated for 7 days under light intensity of 1500lux, 30°C and anaerobic conditions. The culture was...

Embodiment 2

[0030] Prepare media with the following components: MgSO 4 ·7H 2 O300mg, CaCl 2 2H 2 O100mg, sodium acetate 2500mg, EDTA30mg, yeast extract powder 1500mg, K 2 HPO 4 1500mg, (NH 4 ) 2 SO 4 2000mg, FeSO 4 ·7H 2 O15mg, KH 2 PO 4 1000mg, trace element solution 5ml, deionized water 1500ml, pH 7.

[0031] Wherein the trace element solution is composed of: H 3 BO 3 240mg, MnSO 4 4H 2 O200mg, Na 2 MoO 4 2H 2 O70mg, CuSO 4 7mg, ZnSO 4 ·7H 2 O24mg, deionized water 200ml.

[0032] Insert 1 / 20 of the photosynthetic bacteria into the sterilized culture medium, and cultivate it for 7 days under light intensity of 1500lux, 35°C and anaerobic conditions.

[0033] The above-mentioned highly active photosynthetic bacteria liquid was inoculated into 2.0mmol / L selenite medium containing 20% ​​of the total amount, and cultivated for 9 days under light intensity of 1500lux, 35°C and anaerobic conditions. Other conditions are identical with embodiment 1.

Embodiment 3

[0035] The highly active photosynthetic bacteria in Example 1 were used to centrifuge at 8000×g for 10 min, wash the precipitate with physiological saline and centrifuge twice. Then weigh the wet cells of photosynthetic bacteria (embedding amount 30%) and mix them with PVA (mass concentration 10%) solution cooled to 35°C, and drop this mixture into the saturated boric acid solution with pH 6.5 with a syringe to form Small balls around 3mm. The formed pellets were immobilized in a refrigerator at 4°C for 18 hours, and then activated in the culture medium for 6 hours to prepare immobilized cells for use.

[0036] Sterilize 1000ml of photosynthetic bacteria culture medium containing 2.0mmol / L selenite at 121°C for 30 minutes, cool to room temperature, add immobilized bacteria (inoculated at 20%), and in anaerobic, light intensity 1000lux, After cultivating at 30°C for 7 days, the immobilized pellets were taken out to purify the red elemental selenium. The steps were the same as ...

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Abstract

The invention discloses a method for preparation of red elemental selenium by photosynthetic bacteria, and particularly provides a method for reducing Se4<+> to red elemental selenium by photosynthetic bacteria under anaerobic light conditions. The steps include: directly inoculating well activated free photosynthetic bacteria or immobilized photosynthetic bacteria into a photosynthetic bacteria culture medium containing selenite, conducting culture under certain temperature and lighting anaerobic conditions, and then carrying out separation, washing and drying, thus obtaining the red elemental selenium. The red elemental selenium synthesized by photosynthetic bacteria under anaerobic light conditions in the invention has the characteristics of small particle size, uniform distribution, good dispersibility, and high purity. The process for production of red elemental selenium by the bacteria has the advantages of safety, reliability, no pollution, easily controllable reaction conditions, and low production cost, thus being suitable for large scale production.

Description

technical field [0001] The invention relates to a method for preparing red elemental selenium, in particular to a method for preparing red elemental selenium by using a microbial reduction method. Background technique [0002] Selenium (Se) element, first discovered by the Swedish scientist Berzelius in 1817, belongs to the sixth main group. It is an ultra-trace element in geochemistry and has long been regarded as a toxic element. In 1957, Schwarz et al. confirmed that selenium can promote animal growth and prevent liver necrosis in rats. Prior to this, people's research on selenium has only focused on its toxic effects. In 1973, the World Health Organization (WHO) announced that selenium is an essential trace element in human and animal life. In 1988, the Chinese Nutrition Society listed selenium as one of the 15 daily dietary nutrients necessary for the human body. Selenium mainly exists in the human body in the form of selenocysteine, which can effectively increase th...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/01
Inventor 李保珍王兰张肇铭
Owner SHANXI UNIV
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