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Application of composition of bacillus subtilis BS01 and ganoderma lucidum spore powder in improving sleep

A technology of Bacillus subtilis and Ganoderma lucidum spore powder, which is applied in the direction of drug combination, medical preparations containing active ingredients, bacteria, etc. It can solve the problems of unseen, sleep improvement, unseen Bacillus subtilis combined with Ganoderma lucidum spore powder, etc.

Active Publication Date: 2013-10-23
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no related reports on improving sleep with Bacillus subtilis combined with Ganoderma lucidum spore powder, and no related products have been seen.

Method used

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  • Application of composition of bacillus subtilis BS01 and ganoderma lucidum spore powder in improving sleep
  • Application of composition of bacillus subtilis BS01 and ganoderma lucidum spore powder in improving sleep
  • Application of composition of bacillus subtilis BS01 and ganoderma lucidum spore powder in improving sleep

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Collection and Separation of Samples

[0017] Take 0.5 g of soil and put it into a conical flask with 49.5 ml of normal saline and glass beads, shake it on a shaker at 37°C for 20 minutes, and then bathe in a constant temperature water bath at 60°C for 20 minutes. Take 0.5ml of soil dilution solution and make a tenfold dilution to 10 -5 . from 10 -3 ~10 -5 100 μL of each dilution was spread on ordinary nutrient agar medium, and incubated at a constant temperature of 37°C for 24h. After staining and examining the single colony under the microscope, pick a single colony of Bacillus and line it on an ordinary nutrient agar plate for culture. Repeat the line drawing of each single colony on the plate 2 to 3 times until a pure strain is obtained, which is numbered as Bacillus BS01. The obtained Bacillus BS01 was inoculated on a nutrient agar slant and preserved for future use.

Embodiment 2

[0018] Example 2 Bacillus BS01 colony characteristics

[0019] Bacillus BS01 of the present invention is cultured by streaking in LB medium, and it can be seen that the colony is round, with moist surface, irregular edges, off-white or yellowish, and a single colony is picked for Gram staining, and the short bacilli appear purple after staining. See middle-born or partial-born spores, indicating that the bacterial strain of the present invention is a Gram-positive bacillus.

Embodiment 3

[0020] Example 3 The acid resistance experiment of bacillus BS01

[0021] Cultivate Bacillus BS01 in LB medium overnight at 37°C and collect the bacteria by centrifugation at 3000r / min, suspend the bacteria in 0.05M phosphate buffer solution-pH2.0 and cultivate for 1-3 hours, and dynamically detect the number of viable bacteria during the culture period Variety. The experimental results showed that the survival rate reached 81.24% after 3 hours in the pH 2.0 buffer solution (see Table 1). It shows that the bacterial strain BS01 of the present invention has stronger acid resistance.

[0022] Table 1 The acid resistance test Log of Bacillus BS01 10 CFU / mL

[0023] strain 0h 1h 2h 3h Survival rate (%) Bacillus BS01 9.01 7.98 7.54 7.32 81.24

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Abstract

The invention discloses an application of a composition of bacillus subtilis BS01 and ganoderma lucidum spore powder in improving sleep. According to the invention, with the combination of bacillus subtilis BS01 and ganoderma lucidum spore powder, mice sleep latency time and serum MDA content can be substantially reduced. Mice sleep time, subliminal hypnotic sleep rate, CAT activity, GSH-Px activity and SOD activity are respectively improved by 54.36%, 43.75%, 98.13%, 49.27% ??and 12.61%. With the composition of bacillus subtilis BS01 and ganoderma lucidum spore powder, mice sleep quality is effectively improved. The composition can be used for preparing biological products used for improving sleep.

Description

technical field [0001] The invention relates to the application of the composition of bacillus subtilis BS01 and ganoderma lucidum spore powder in improving sleep. Background technique [0002] In the 21st century, with the intensification of social competition and the acceleration of the pace of life, the incidence of insomnia is on the rise. Medical research shows that insomnia can bring about consequences such as anxiety, depression, inattention, and memory impairment, which have a serious impact on the lives of patients. [0003] Therefore, the research and development of biological products that improve sleep is of great significance to prevent diseases and protect human physical and mental health. However, the current methods of improving sleep are mostly focused on diazepam, melatonin, benzodiazepines, non-benzodiazepines (such as zaleplon, zolpidem, trazodone, etc.), cytokines and plant hormones. extracts etc. Although these substances have a certain effect of im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K36/074A61P25/20C12R1/125A61K35/74A61K35/742
Inventor 曾东倪学勤闫冰周梦佳曾燕李倩李涛
Owner SICHUAN AGRI UNIV
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