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Fermentation and coating of lysine and compound enzyme

A lysine and amino acid technology, applied in the direction of enzymes, enzymes, animal feed, etc., can solve the problems of weakened enzyme activity, easy mixing and unevenness, troubles, etc.

Active Publication Date: 2013-10-23
NINGXIA EPPEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is more troublesome to use, and it is easy to mix unevenly, which may make the corresponding utilization rate low
[0006] The inventor imagines that xylanase and / or cellulase are directly mixed with lysine fermentation broth in the process of preparing coated lysine products, which can be mixed evenly and then coated, however However, it is found that due to the high temperature and drying process to be experienced in the coating, the conventional enzyme activity will be greatly weakened or even completely lost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Preparation of expression construct for heat-resistant and dehydration-resistant xylanase gene

[0058] According to the strain we found, we commissioned the Institute of Microbiology, Chinese Academy of Sciences to conduct sequence analysis, and found a new xylanase gene, its nucleotide sequence is shown in SEQ ID No: 1 of the sequence table, and the encoded xylan The amino acid sequence of the enzyme is shown in SEQ ID No:2. Then, according to the "Molecular Cloning Experiment Guide" and the operating guide of the commercial reagents used to construct the secretory expression of the yeast, in short, the gene encoding the xylanase with a forward primer such as SEQ ID No: 5 of the sequence listing As shown (the EcoR I endonuclease site was introduced), the reverse primer was shown in SEQ ID No: 6 of the sequence listing (the Not I endonuclease site was introduced) and EcoR I and Not I double enzymes were used after amplification and ligated with the p...

Embodiment 2

[0060] Example 2 Fermentation preparation and activity determination of heat-resistant and dehydration-resistant xylanase

[0061] Inoculate the positive yeast transformant obtained in Example 1 into 50ml BMGY liquid medium (100mM potassium phosphate buffer (pH6.0), containing 1% yeast extract, 2% peptone, 1.34% yeast nitrogenous base, biological Prime 4*10 -5 %, glucose 2%, glycerol 1%), cultured at 30°C, 200rpm until OD600 reached 5.0.

[0062] Transfer the above culture to 1L BMGY liquid medium, cultivate at 30°C, 200rpm for 24 hours, add 5ml of methanol, and then continue to cultivate at 30°C, 200rpm for 8 days, during which 5ml of methanol was added every 24 hours, and the solution was controlled by ventilation. Oxygen (DO) is greater than 20%, and the pH is controlled at 6.0 (adjusted with ammonia water). After the cultivation is completed, filter with a 0.22 μm filter membrane and retain the supernatant, which is detected by SDS-PAGE to be rich in proteins correspon...

Embodiment 3

[0066] Example 3 Preparation of expression construct of heat-resistant and dehydration-resistant cellulase gene

[0067] According to the bacterial strain we found, we commissioned the Institute of Microbiology, Chinese Academy of Sciences to conduct sequence analysis, and found a new cellulase gene, its nucleotide sequence is shown in SEQ ID No: 3 of the sequence table, the encoded cellulase The amino acid sequence is shown in SEQ ID No:4. Then, according to the "Molecular Cloning Experiment Guide" and the operating guide of the commercial reagents used to construct the secretory expressed yeast, in short, the gene encoding the cellulase is used as a forward primer as shown in SEQ ID No: 7 of the sequence listing shown (the EcoR I endonuclease site was introduced), the reverse primer was shown in SEQ ID No: 8 of the sequence table (the Not I endonuclease site was introduced) and double-digested with EcoR I and Not I after amplification , and ligated with the pPIC3.5...

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PUM

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Abstract

The invention provides a method for preparation of an L-lysine product by fermentation. An L-lysine fermentation solution is produced by fermentation; the L-lysine fermentation solution is mixed with high temperature-resistant dehydrated compound enzyme obtained by fermentation; and the mixture is dried and is coated by fat. The invention also provides a product and an enzyme obtained by the method.

Description

technical field [0001] The invention belongs to the field of fermentation of amino acids. Specifically, the invention relates to a method for fermenting and preparing L-lysine products, which includes fermenting and producing L-lysine fermentation broth, which can be dehydrated with fermentable high-temperature resistant Xylanase and cellulase are mixed, dried at high temperature and coated with fat. In addition, the present invention also provides products produced by the method and the like. Background technique [0002] Xylanase and cellulase can effectively degrade lignin and cellulose-containing substances (such as feed), so adding xylanase and cellulase to feed can enhance the absorption and utilization of feed, thereby saving feeding costs . For example, Chinese patent 96196760.9 discloses an enzyme supplement for ruminant feed, which contains xylanase and cellulase. [0003] L-lysine is an important amino acid raw material, which can be used as condiment, food, fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/00C12N9/42
Inventor 马吉银陈崇安孟刚曹洪程耀东刘鑫
Owner NINGXIA EPPEN BIOTECH
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