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Method for preparing lysine fermentation culture medium by utilizing mycoprotein

A technology for lysine fermentation and cell protein, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high cost of lysine fermentation medium, low lysine yield, and cell Problems such as low added value of protein, to achieve the effect of improving protein solubility and hydrophilicity, increasing mass transfer rate, and facilitating subsequent enzymatic hydrolysis

Inactive Publication Date: 2020-03-17
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects of low added value of bacterial protein, high cost of lysine fermentation medium and low yield of lysine in the prior art, and provide a method for preparing lysine fermentation medium by using bacterial protein

Method used

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  • Method for preparing lysine fermentation culture medium by utilizing mycoprotein
  • Method for preparing lysine fermentation culture medium by utilizing mycoprotein
  • Method for preparing lysine fermentation culture medium by utilizing mycoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Utilize the method for preparing lysine fermentation medium with bacterial protein, it comprises the steps:

[0037] Take each raw material according to the following concentrations: bacterial protein distiller's grains joint hydrolyzate 200ml / L, glucose 40g / L, molasses 12g / L, ammonium sulfate 4g / L, sodium citrate 5g / L, potassium dihydrogen phosphate 10g / L, seven Magnesium sulfate water 0.8g / L, manganese sulfate tetrahydrate 0.02g / L, ferrous sulfate heptahydrate 0.02g / L, vitamin B 1 0.01g / L, biotin 0.5mg / L;

[0038] Add each raw material to water in turn, stir evenly, adjust the pH to 7.8 with 100g / L NaOH aqueous solution, and sterilize at 115°C for 10min to obtain the product.

[0039] The improvements of the fermentation medium of the present invention relative to the conventional medium mainly include:

[0040] Conventional medium: glucose 80g / L, corn steep liquor 10g / L, molasses 12g / L, ammonium sulfate 8g / L, potassium dihydrogen phosphate 10g / L, magnesium sulfate h...

Embodiment 2

[0051] Utilize the method for preparing lysine fermentation medium with bacterial protein, it comprises the steps:

[0052] Take each raw material according to the following concentrations: bacterial protein distiller's grains joint hydrolyzate 200ml / L, glucose 40g / L, molasses 12g / L, ammonium sulfate 4g / L, sodium citrate 5g / L, potassium dihydrogen phosphate 10g / L, seven Magnesium sulfate water 0.8g / L, manganese sulfate tetrahydrate 0.02g / L, ferrous sulfate heptahydrate 0.02g / L, vitamin B 1 0.01g / L, biotin 0.5mg / L;

[0053] Add each raw material to water in turn, stir evenly, adjust the pH to 7.8 with 100g / L NaOH aqueous solution, and sterilize at 115°C for 10min to obtain the product.

[0054] The preparation method of the cell protein distiller's grains joint hydrolyzate is as follows:

[0055] Grind and pulverize the bacterial protein, then add it to 8 times the weight of a 0.5M hydrochloric acid solution, stir evenly, then place it in a high-speed shearing machine at a s...

Embodiment 3

[0059] Utilize the technology of fermenting and producing lysine in fermentation medium, it comprises the steps:

[0060] The liquid volume in the 50L automatic fermentation tank is 30L fermentation medium, and the strain is Brevibacterium flavum XQ90; the Brevibacterium flavum is inserted into the fermentation medium according to the inoculation amount of 5%, the inoculation concentration is 2g / L, and the fermentation temperature is 0 -18h is 31°C, 18h-end is 34°C, the ventilation ratio is 1:0.7, the stirring speed is 300r / min, and the total fermentation time is 60h; during the fermentation process, the residual sugar content is maintained at 5 -10g / L, maintain ammonia nitrogen content at 1-2g / L by feeding 300g / L ammonium sulfate solution, feed ammonia water to control pH7.0-7.2, and feed defoamer to defoam.

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Abstract

The invention belongs to the technical field of amino acid fermentation. The invention discloses a method for preparing a lysine fermentation culture medium by utilizing mycoprotein. The method comprises the steps of 1) taking the following raw materials: mycoprotein vinasse combined hydrolysate, glucose, molasses, ammonium sulfate, sodium citrate, monopotassium phosphate, magnesium sulfate heptahydrate, manganese sulfate tetrahydrate, ferrous sulfate heptahydrate, vitamin B1 and biotin; and (2) sequentially adding the raw materials into water, uniformly stirring, adjusting the pH value to 7.8by using a NaOH aqueous solution, and sterilizing for 10 minutes at 115 DEG C. According to the method disclosed by the invention, the lysine fermentation culture medium is prepared by using mycoprotein and vinasse as main raw materials, so that the cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a method for preparing a lysine fermentation medium by using bacterial protein. Background technique [0002] Lysine, also known as L-lysine, lysine, etc., is one of the most important essential amino acids in human and animal nutrition. At present, the global production of lysine has reached more than 2.4 million tons, second only to glutamic acid , becoming the second largest amino acid variety in the world. The lysine slag is a new bacterial protein feed product produced by microbial fermentation with lysine waste liquid as raw material, accompanied by the production of lysine. Lysine waste liquid contains a large amount of: bacterial protein, COD, BOD, reducing sugar, lysine, alanine, aspartic acid, leucine, etc., in addition, it also contains trace amounts of P, K, Ca, etc. Inorganic salt. [0003] Lysine derivatives developed earlier abroad. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12N1/20C12R1/13
CPCC12P13/08C12N1/20
Inventor 汲广习唐永强关健董力青李航韩杨
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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