Method for enhancing fermentation yield of lysine
A technology for lysine fermentation and yield, applied in the field of microbial fermentation, can solve the problem of high cost of glucose, and achieve the effects of low price, easy crushing, and increased yield
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Embodiment 1
[0019] Embodiment 1 conventional lysine fermentation production method
[0020] Conventional seed tank medium is: glucose 25g / L, corn steep liquor 3g / L, KH 2 PO 4 ·3H 2 O15g / L, MgSO 4 ·7H 2 O 0.4g / L, urea 4g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O 0.01g / L, the culture conditions used for seed tank culture are: inoculum size 1‰, temperature 37°C, ventilation ratio 1:0.5vvm, tank pressure 0.05Mpa, full control of DO30% or more, pH6.90, cycle 12h.
[0021] The seed tank seed liquid of conventional culture is inserted into conventional fermenter culture medium (glucose 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O 4.5g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.015g / L, bean cake hydrolyzate 30g / L, vitamin H 150μg / L, vitamin B11mg / L, pH 6.5-7.0; sterilized at 121°C for 30 minutes and cooled to 37°C), at a pressure of 0.1MPa and an air volume of 0.3- 0.8vvm, dissolved oxygen 30-60% under the condition of fermentation culture. 13h after ...
Embodiment 2
[0022] Embodiment 2 improves the method for lysine fermentation yield
[0023] The seed tank medium for cassava liquefaction liquid culture is: cassava liquefaction liquid 100g / L, corn steep liquor 3g / L, KH 2 PO 4 ·3H 2 O 15g / L, MgSO 4 ·7H 2 O 0.4g / L, urea 4g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O 0.01g / L, the culture conditions used for seed tank culture are: inoculum size 1‰, temperature 37°C, ventilation ratio 1:0.5vvm, tank pressure 0.05Mpa, full control of DO30% or more, pH6.90, cycle 13h.
[0024] The seed tank seed liquid cultivated with cassava liquefaction liquid is inserted into the fermenter culture medium (cassava liquefaction liquid 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O 4.5g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.015g / L, MnSO 4 ·H2 O 0.015g / L, bean cake hydrolyzate 30g / L, vitamin H 150μg / L, vitamin B11mg / L, pH 6.5-7.0; sterilized at 121°C for 30 minutes and cooled to 37°C), at a pressure of 0.1MPa and an air volume of 0.3- 0.8vvm,...
Embodiment 3
[0025] Embodiment 3 improves the method for lysine fermentation yield
[0026] The seed tank medium for cassava liquefaction liquid culture is: cassava liquefaction liquid 100g / L, corn steep liquor 3g / L, KH 2 PO 4 ·3H 2 O 15g / L, MgSO 4 ·7H 2 O 0.4g / L, urea 4g / L, FeSO 4 ·7H 2 O 0.01g / L, MnSO 4 ·H 2 O 0.01g / L, the cultivation conditions adopted for primary seed tank cultivation are: inoculum size 1‰, temperature 37°C, ventilation ratio 1:0.5vvm, tank pressure 0.05Mpa, full control of DO30% or more, PH6.90, cycle 13h.
[0027] The seed tank seed liquid cultivated with cassava liquefaction liquid is inserted into the fermenter culture medium (cassava liquefaction liquid 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O 4.5g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.015g / L, bean cake hydrolyzate 30g / L, vitamin H 150μg / L, vitamin B11mg / L, pH 6.5-7.0; sterilized at 121°C for 30 minutes and cooled to 37°C), at a pressure of 0.1MPa and an air v...
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