Analysis method of lysine fermentation liquid

Abstract

The invention discloses an analysis method of a lysine fermentation liquid. The lysine fermentation liquid contains lysine, fermentation strains, reducing sugar and amino nitrogen. The method comprises the steps as follows: near-infrared spectrograms of multiple standard samples of the lysine fermentation liquid are acquired; the content of the lysine, OD (optical density) values of the fermentation strains, the content of the reducing sugar and the content of the amino nitrogen in the multiple standard samples are obtained respectively; multiple linear regression equations of the content of the lysine, the OD values of the fermentation strains, the content of the reducing sugar and the content of the amino nitrogen as well as respective corresponding near infrared absorbance values are obtained respectively; and the content of the lysine, the OD values of the fermentation strains, the content of the reducing sugar and the content of the amino nitrogen in to-be-tested samples of the lysine fermentation liquid are calculated. According to the technical scheme, the purpose of synchronous, quick and safe measurement of the content of the lysine, the OD value of the fermentation strains, the content of the reducing sugar and the content of the amino nitrogen in the lysine fermentation liquid is achieved.

Description

technical field [0001] The invention relates to a method for analyzing lysine fermentation liquid. Background technique [0002] In the fermentation of lysine, lysine is the main metabolite, and its content is an important indicator for judging the level of fermentation; the fermentation of lysine mainly uses fermentation strains to convert sugar into lysine, and the fermentation strains The growth condition directly affects the yield of lysine. During the fermentation process, the OD value of the fermented strains is usually used to understand the density distribution of the fermented strains in the fermentation broth, and to judge the growth stage of the fermented strains to adjust the supply. Oxygen and nutrients, so the OD value of the fermentation strain is an important indicator to measure the growth of the fermentation strain; reducing sugar and amino nitrogen generally exist in the fermentation broth as the carbon source and nitrogen source of the fermentation strain...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of prior art determination of lysine content in lysine fermentation broth, OD value of fermented bacteria, reducing sugar content and amino nitrogen content, time-consuming and high risk , to provide an analytical method for lysine fermentation broth capable of synchronously, rapidly and safely determining the content of lysine in the fermentation broth of lysine, the OD value of the fermentation strain, the content of reducing sugar and the content of amino nitrogen

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