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Method for strong seedling and rooting culture of strawberries

A technology for rooting and cultivating seedlings, applied in the field of botany, can solve the problems of strawberry seedling germplasm degradation and the like, and achieve the effects of improving yield and quality, enhancing economic value, and growing robust seedlings

Active Publication Date: 2013-10-16
句容市春城镇年胜葡萄园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of problem that can effectively solve the problem of germplasm degradation of strawberry seedlings, and improve the survival rate and yield of strong strawberry seedling rooting culture method

Method used

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Experimental program
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specific Embodiment approach

[0017] In this embodiment, the modified MS used in all examples includes macronutrients, micronutrients and organic reagents,

[0018] And the components of the macronutrient elements and their corresponding concentrations are: potassium nitrate 1800 mg / L; ammonium sulfate 1880 mg / L; calcium chloride dihydrate 360 ​​mg / L; anhydrous potassium dihydrogen phosphate 150 mg / L ; Disodium edetate 32.5 mg / L; Ferrous sulfate heptahydrate 25.3 mg / L,

[0019] The components of the micronutrient elements and their corresponding concentrations are: manganese sulfate tetrahydrate 18.5 mg / L; zinc sulfate 9.5 mg / L; boric acid 7.5 mg / L; potassium iodide 1.5 mg / L; sodium molybdate 0.5 mg / L L; cobalt chloride 0.03 mg / L,

[0020] The components of the organic reagent and their corresponding concentrations are: thiamine hydrochloride 15.0 mg / L; nicotinic acid 2.0 mg / L; pyridoxine hydrochloride 2.0 mg / L; inositol 80.0 mg / L.

Embodiment 1

[0022] The strong seedling rooting culture method of strawberry of the present invention is specifically:

[0023] (1) Rooting culture of strong seedlings:

[0024] Cut the bushy shoots with one original leaf that grow robustly to 0.3-0.5cm into individual plants and transfer them to the rooting medium for strong seedlings of strawberry, which includes improved MS + 0.2mgL -1 IBA+0.01mgL -1 NAA +0.8mg indole butyric acid +0.2 gL -1 Activated carbon+5.0gL -1 Agar + 10gL -1 Sucrose, and the pH value is 5.2, and keep the temperature at 20-25°C, the light time is 15-20h, culture for 5 weeks, the stems and leaves develop normally, the root system grows robustly, and when the plant grows to 3-5cm, transplant it;

[0025] (2) Transplanting:

[0026] Transplant the plants described in step (1) into the cultivation room, wash off the residual culture medium at the base with clean water, and keep the indoor temperature at 20-25°C. After 30 days, transplant them into a shade with a...

Embodiment 2

[0028] The strong seedling rooting culture method of strawberry of the present invention is specifically:

[0029] (1) Rooting culture of strong seedlings:

[0030] Cut the bushy shoots with one original leaf that grow robustly to 0.3-0.5cm into individual plants and transfer them to the rooting medium for strong seedlings. The rooting medium for strong strawberry seedlings includes improved MS + 0.3mgL -1 IBA+0.03mgL -1 NAA +0.5mg indole butyric acid +0.4 gL -1 Activated carbon+4.0gL -1 Agar + 10gL -1 Sucrose, and the pH value is 5.5, and the temperature is kept at 20-25°C, and the light time is 15-20h. After 10 weeks of cultivation, the stems and leaves develop normally, and the root system grows robustly. When the plant grows to 3-5cm, it is transplanted;

[0031] (2) Transplanting:

[0032] Transplant the plants described in step (1) into the cultivation room, wash off the residual medium at the base with water, and keep the indoor temperature at 20-25°C. After 35 da...

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Abstract

The invention discloses a method for strong seedling and rooting culture of strawberries, which includes the following steps: firstly, strong seedling and rooting culture: a multiple shoot with a prophyll and 0.3-0.5 cm in height and well in growth and development is cut into a single plant and transplanted to a strong seedling and rooting culture medium for rooting culture, and transplanting is carried out after the plant is 3-5 cm in height; secondly, transplantation: the plant is firstly transplanted to a culture room and grows for 30-35 d, then is transplanted in a plastic-covered tunnel with a shading net, after a new root grows and the plant sprouts, watering frequency is gradually reduced, and the plant is transplanted to a flowerpot for ordinary management. The method for strong seedling and rooting culture of strawberries, provided by the invention, improves the survival rate of transplanted plants by 90% above through supporting measures such as improvement on minimal medium and ingredients and adjustment in culture conditions, enables seedlings to grow healthy, effectively solves the problem of genetical characterization decline of fine seedlings, effectively improves yield and quality of strawberries and enhances the economic value of strawberries at the same time.

Description

technical field [0001] The invention relates to a method for rooting and cultivating strong strawberry seedlings, which belongs to the technical field of botany. Background technique [0002] In the production of strawberries, the rooting process of strong seedlings is very critical. It needs to be free from diseases and insect pests, and the number of leaves is reasonable, the color is bright green, the rhizomes are developed, and the thickness is uniform. However, traditional strawberries are prone to plant diseases and insect pests during the rooting and strong seedling stage, causing the root system to degenerate and affecting the Survival rate and strawberry yield, therefore, be badly in need of a kind of method of tissue culture to carry out tissue culture and take root strong seedling to it. Contents of the invention [0003] In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a method for rooting and cultivatin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 毕年胜芮东明
Owner 句容市春城镇年胜葡萄园
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