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Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)

A biological activity, glucagon technology, which is applied in the field of glucagon-like peptide-1 biological activity detection reagents, detection methods and kits, can solve problems such as short retention time, and achieves simple and practical methods. Strong and sensitive effect

Active Publication Date: 2013-10-09
斯坦姆(天津)生物技术研究有限公司
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Problems solved by technology

Aiming at the limitation that GLP-1 analogues stay in the body for a short time in clinical practice and require daily injections, a GLP-1 mutant polypeptide with a longer half-life is provided. The GLP-1 mutant polypeptide has a longer half-life and does not Patients need to be injected every day, which can effectively improve the patient's compliance attitude

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  • Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
  • Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
  • Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)

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Embodiment Construction

[0056] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0057] The invention establishes a method for measuring the biological activity of GLP-1 based on receptor-ligand combination, a corresponding detection reagent and a kit. The present invention clones the target gene of GLP-1 receptor, and establishes a cell line expressing GLP-1 receptor through gene recombination technology, thereby obtaining purified GLP-1 receptor, GLP-1 and GLP-1 in the serum (or plasma) to be tested The GLP-1 receptor on the cell membrane binds, the receptor is activated to produce the second messenger cAMP, and the cAMP content in the supernatant is detected with a commercial cAMP kit, thus reflecting the biological content of GLP-1 in the serum (or plasma) to be tested learning activity. At the same time, a self-con...

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Abstract

The invention relates to a reagent for detection of biological activity of glucagon-like peptide-1 (GLP-1). Each 100 microliters of the reagent mainly comprises 20 micrograms of a gene recombinant GLP-1 acceptor, a dipeptidyl peptidase-4 inhibitor of which the weight is 1% of the weight of a blood plasma sample, 480 micrograms of triphosadenine, 10 micrograms of guanosine triphosphate, 0.5 milligrams of 3-isobutyl-1-methylxanthine and 100 microliters of a membrane reaction solution. The GLP-1 acceptor is obtained by genetic recombination, is convenient for purification and reduces batch difference of the reagent. The GLP-1 acceptor is used as a probe for capturing GLP-1 so that detection sensitivity is improved, biological activity can be directly determined, sensitivity is high, and practicality is strong.

Description

technical field [0001] The invention relates to a biological detection reagent, in particular to a biological activity detection reagent of glucagon-like peptide-1 (GLP-1), a detection method and a kit. Background technique [0002] Diabetes is based on polygenic inheritance, coupled with the effects of environmental factors and autoimmunity, through an incompletely elucidated mechanism, causing insulin secretion disorders and insufficient insulin biological effects, resulting in hyperglycemia as the basic biochemical characteristics of sugar, A group of clinical syndromes characterized by disorders of fat, protein, water and electrolyte metabolism. In typical cases, symptoms of "three excesses and one deficiency" such as polyuria, polydipsia, polyphagia, and weight loss may appear. The chronic complications are mainly specific and non-specific microangiopathy and peripheral neuropathy. At present, diabetes has become the third major non-communicable disease after tumors an...

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Application Information

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IPC IPC(8): G01N33/74C12N15/12C12N15/85C07K14/72
Inventor 李迺昶李会强周宇捷任峰
Owner 斯坦姆(天津)生物技术研究有限公司
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