Reagent, method and kit for detection of biological activity of glucagon-like peptide-1 (GLP-1)
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of glucagon and biological activity, which is applied in the field of detection reagents, can solve the problems of short retention time and achieve the effect of preventing the loss of cAMP, strong practicability and simple method
Active Publication Date: 2014-11-26
MEIDE TAIPINGYANG PRECISION INSTR MFG
View PDF10 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Aiming at the limitation that GLP-1 analogues stay in the body for a short time in clinical practice and require daily injections, a GLP-1 mutant polypeptide with a longer half-life is provided. The GLP-1 mutant polypeptide has a longer half-life and does not Patients need to be injected every day, which can effectively improve the patient's compliance attitude
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0059] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.
[0060] The invention establishes a method for measuring GLP-1 biological activity based on receptor-ligand binding, and directly captures cAMP secreted after receptor activation, and corresponding detection reagents. The present invention clones the target gene of GLP-1 receptor, and establishes a cell line expressing GLP-1 receptor through gene recombination technology, thereby obtaining purified GLP-1 receptor, GLP-1 and GLP-1 in the serum (or plasma) to be tested The GLP-1 receptor on the cell membrane binds, the receptor is activated to produce the second messenger cAMP, the microwell plate coated with goat anti-human cAMP polyclonal antibody directly captures the cAMP produced, and then adds biotin-labeled mouse anti-human The cAMP mono...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a reagent for detection of biological activity of glucagon-like peptide-1 (GLP-1). The reagent added into each 15 microliters of a detection sample comprises: 1, 35-45 microliters of a membrane reaction solution, 2, 15-25 microliters of a GLP-1 acceptor, 3, 10-20 microliters of a GLP-1 standard substance, 4, 5-15 microliters of sitgliptin phosphate, 5, 1-10 microliters of triphosadenine, 6, 1-10 microliters of guanosine triphosphate, 7, 5-15 microliters of 3-isobutyl-1-methylxanthine, 8, 1-10 microliters of bovine serum albumin, 9, 50-150 microliters of a biotin-labeled mouse anti-human cAMP monoclonal antibody, 10, a microwell plate coated with a goat anti-human cAMP polyclonal antibody, 11, 50-150 microliters of an avidin-labeled horseradish peroxidase, 12, 300-400 microliters of a PBS washing liquor, 13, 50-150 microliters of a chromogenic substrate, and 14, 50-150 microliters of a stop buffer. The GLP-1 acceptor is produced by genetic recombination, is convenient for purification and can reduce the batch difference of the reagent. The GLP-1 acceptor is used as a probe for capturing GLP-1 so that detection sensitivity is improved, biological activity can be directly determined, sensitivity is high, and practicality is strong.
Description
technical field [0001] The invention relates to a detection reagent in the field of medical testing, in particular to a detection reagent, detection method and kit for glucagon-like peptide-1 (glucagon-like peptide-1, GLP-1) biological activity. Background technique [0002] Diabetes is based on polygenic inheritance, coupled with the effects of environmental factors and autoimmunity, through an incompletely elucidated mechanism, causing insulin secretion disorders and insufficient insulin biological effects, resulting in hyperglycemia as the basic biochemical characteristics of sugar, A group of clinical syndromes characterized by disorders of fat, protein, water and electrolyte metabolism. In typical cases, symptoms of "three excesses and one deficiency" such as polyuria, polydipsia, polyphagia, and weight loss may appear. The chronic complications are mainly specific and non-specific microangiopathy and peripheral neuropathy. At present, diabetes has become the third maj...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.