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Method for building in-situ model of transplantable mouse breast cancer tumor tissue block

A breast cancer tumor and orthotopic model technology, which is applied in the field of establishing an in situ model of mouse transplantable breast cancer tumor tissue block, can solve the problems of quantification, inoculation time, difficulty in effectively controlling tumor model tumor formation rate, transplantation rejection, etc.

Inactive Publication Date: 2013-10-02
BENGBU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the orthotopic model of transplanted tumor is easy to quantify and the limitation of inoculation time is difficult to effectively control the tumor formation rate and cycle of the tumor model required for the experiment, and the insurmountable transplant rejection among various animal strains is urgently needed. Reproducible orthotopic models of transplanted tumors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The orthotopic model of transplantable breast cancer tumor tissue blocks in mice was established according to the following steps:

[0020] a) Spontaneous breast cancer tumors in mice were excised under sterile conditions, and washed 3 times with sterile phosphate buffered saline;

[0021] b) Segment the tumor mass into volumes of 0.7mm 3 Inoculate tumor mass;

[0022] c) Select 20 C from Group A 3 For H mice, cut a small opening in the armpit of the mouse forelimb, use ophthalmic tweezers without hooks to pick up a small piece, send it into the incision subcutaneously, and inoculate the tumor mass in the subcutaneous area of ​​the mouse forelimb armpit;

[0023] d) Breeding under conventional conditions, normal reproduction for 21 days, and the establishment of a mouse transplantable breast cancer orthotopic model was completed.

[0024] 7-9 days after the inoculation of 20 mice in group A after the above steps, 17 of them formed local tumor nodules, and 17 of them ...

Embodiment 2

[0026] The orthotopic model of transplantable breast cancer tumor tissue blocks in mice was established according to the following steps:

[0027] a) Spontaneous breast cancer tumors in mice were excised under aseptic conditions, and washed 4 times with sterile phosphate buffered saline;

[0028] b) Divide the tumor block into inoculated tumor blocks with a volume of 0.9mm3;

[0029] c) Select 20 C from group B 3 For H mice, cut a small opening in the armpit of the mouse forelimb, use ophthalmic tweezers without hooks to pick up a small piece, send it into the incision subcutaneously, and inoculate the tumor mass in the subcutaneous area of ​​the mouse forelimb armpit;

[0030] d) Breeding under conventional conditions, normal reproduction for 21 days, and the establishment of a mouse transplantable breast cancer orthotopic model was completed.

[0031] 7-9 days after the inoculation of 20 mice in group B after the above steps, 18 of them formed local tumor nodules, and 18 o...

Embodiment 3

[0033] The orthotopic model of transplantable breast cancer tumor tissue blocks in mice was established according to the following steps:

[0034] a) Spontaneous breast cancer tumors in mice were excised under sterile conditions, and washed 3 times with sterile phosphate buffered saline;

[0035] b) Divide the tumor block into inoculated tumor blocks with a volume of 1.1mm3;

[0036] c) Select 20 C from Group C 3 For H mice, cut a small opening in the armpit of the mouse forelimb, use ophthalmic tweezers without hooks to pick up a small piece, send it into the incision subcutaneously, and inoculate the tumor mass in the subcutaneous area of ​​the mouse forelimb armpit;

[0037] d) Breeding under conventional conditions, normal reproduction for 21 days, and the establishment of a mouse transplantable breast cancer orthotopic model was completed.

[0038] 7-9 days after the inoculation of 20 mice in group C after the above steps, 19 of them formed local tumor nodules, and 19 o...

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Abstract

The invention relates to a method for building an in-situ model of a transplantable mouse breast cancer tumor tissue block. The method comprises steps as follows: a spontaneous mouse breast cancer tumor block is cut into inoculation tumor blocks with volumes ranging from 0.7 mm <3> to 1.9 mm <3>; each tumor block is inoculated under the skin of fore-limb armpits of a mouse without tumor, the mice are raised on normal conditions and normally bred for 9-12 days; and the in-situ model of the transplantable mouse breast cancer is built. According to the method, the spontaneous mouse breast cancer tumor block is cut into the inoculation tumor blocks with smaller volumes, and the tumor blocks are directly inoculated under the skin of the fore-limb armpits of laboratory mice; and the model is simple in building procedure and high in tumor formation rate, has short tumor formation time and no excessive laboratory environment requirements, and can meet requirements of study.

Description

technical field [0001] The invention relates to a method for establishing an in situ model of cancer cells, in particular to a method for establishing an in situ model of a mouse transplantable breast cancer tumor tissue block. Background technique [0002] Breast cancer is the number one common malignant tumor in women. Since the study of this tumor disease cannot be carried out directly in the body of tumor patients, it can be done with the help of experimental animals, usually through C 3 H mice for live animal experiments (C 3 H mice are not immunodeficient compared to immunodeficient mice). [0003] Experimental animal tumor models are an indispensable tool for anticancer drug experiments. There are three kinds of animal models currently used for tumor research: transplanted tumor orthotopic model, spontaneous tumor model and induced tumor model. It is rarely used in the screening experiments of anticancer drugs. [0004] At present, the orthotopic model of transpla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
Inventor 蒋志文刘浩蒋琛琛
Owner BENGBU MEDICAL COLLEGE
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