Tumor targeted living body rapid fluorescence imaging method of rare earth metal nanocluster

A rare earth metal, fluorescence imaging technology, applied in pharmaceutical formulations, preparations for in vivo experiments, etc., can solve the problems of low signal enhancement in vivo, poor probe transport, high biological toxicity, etc., and achieve dynamic tumor targeted therapy. , Real-time tumor targeted therapy, the effect of broad medical application prospects

Active Publication Date: 2013-10-02
SOUTHEAST UNIV
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Problems solved by technology

We also encountered many difficulties in the development of cancer-specific imaging reagents, including: poor transport of probes targeting tissue or tumors; high biological toxicity; poor stability of probes; low signal enhancement in vivo, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0033] Example 1 Imaging method based on in situ biosynthesis of rare earth metal nanoclusters in tumor cells

[0034] 1. Taking HepG2 cells as the research object, incubate a sterile 0.0001mmol / L~1 mmol / L rare earth metal salt solution with HepG2 cells in the logarithmic growth phase for 8~24 hours (37 °C, 5 % CO 2 , RH 95%), the rare earth metal nanoclusters biosynthesized in situ in the cell can be obtained.

[0035] 2. Qualitative and quantitative analysis of rare earth metal compound nanoclusters with confocal fluorescence microscopy;

[0036] Liver cancer cells (HepG2) were selected as the research object, and the experimental group divided the liver cancer cells (HepG2) in the logarithmic growth phase at a ratio of 1.6×10 5 The density of cells / well was inoculated in a 6-well plate, and after 24 h of culture, a rare earth metal salt solution containing a concentration of 0.0001 mmol / L-1 mmol / L was added which had been sterilized and diluted with fresh sterile DMEM m...

example 2

[0038] Example 2 Rapid tumor-targeted imaging method based on in situ growth of rare earth metal nanoclusters in living lesions

[0039] Firstly, 0.1-0.5 mL of sterile rare earth metal salt solution with a concentration of 0.1-100 mmol / L was injected subcutaneously near the tumor of nude mice implanted with liver cancer tumor model, and 4 hours, 8 hours, 12 hours, 12 hours after injection, respectively. Incubate at different time points such as 24 hours and 48 hours, and then anesthetize the experimental nude mice with 5% isoflurane gas, then place them on the operation platform of the small animal in vivo imager, and select the blue light excitation to collect the tumor image of the area.

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Abstract

The invention discloses a tumor targeted living body rapid fluorescence imaging method of a rare earth metal nanocluster. According to the invention, a rare earth metal salt solution of certain concentration and different tumor cells are incubated under physiological conditions for in situ synthesis of the rare earth metal nanocluster, thus realizing real-time, high resolution fluorescence imaging of tumor cells. The rare earth metal nanocluster provided in the invention realizes fast and real-time in situ living tumor targeted fluorescence imaging on a transplant tumor nude mouse model. The rare earth metal nanocluster involved in the invention is biosynthesized directly through tumor cells, and has very good biocompatibility. The in situ living body fluorescence imaging method provided in the invention realizes fast and accurate positioning and tumor targeted imaging analysis, thus having wide medical application prospects.

Description

technical field [0001] The invention relates to a method for preparing a tumor-targeting nanoprobe. Nanobiological probes of rare earth metal nanoclusters with tumor-targeting molecular imaging are grown in situ on living body lesions. Targeted and rapid fluorescence imaging, this in situ in vivo imaging method enables early and rapid diagnosis of tumors. Background technique [0002] Cancer, also known as malignant tumor, has become the biggest killer affecting human health and life in the 21st century. In clinical medical work, the discovery of malignant tumors mainly relies on inspection techniques such as imaging, pathology, and conventional tumor markers. Although these traditional diagnostic methods have been used clinically for many years, they lack sufficient sensitivity and specificity. More importantly, It is difficult to detect tumors early. Early detection of cancerous sites and timely targeted therapy is one of the most effective treatments for cancer. With t...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 王雪梅叶静王建玲
Owner SOUTHEAST UNIV
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