Method for screening and immobilizing efficient petroleum hydrocarbon degradation flora

Abstract

The present invention relates to a method for screening and immobilizing efficient petroleum hydrocarbon degradation flora, wherein the method is applicable for offshore petroleum pollution environment remediation. According to the method, a lot of petroleum hydrocarbon degradation bacterial are enriched, acclimatized and separated from petroleum pollution sea area sediments, the n (n is more than or equal to 3) strains of the bacterial with strong growth activity, different colony morphologies and different cell morphologies are selected, a petroleum hydrocarbon degradation rate of the single strain of the bacterial is determined, 16SrDNA molecule identification is performed on the bacterial, the n strains of the petroleum hydrocarbon degradation bacterial are subjected to mixing culture, the (n-1) strains of the bacterial are randomly selected from the n strains of the bacterial, and are combined to form n groups of different combinations to carry out mixing culture, the optimal combination of the petroleum hydrocarbon degradation bacterial is determined, and the petroleum hydrocarbon degradation flora with the optimal combination is prepared into immobilized bacterial balls to be stored so as to provide a spare use for offshore petroleum pollution environment remediation. The method is an efficient petroleum hydrocarbon degradation flora screening method with characteristics of science, rationality, simplness, feasibility, and meeting of optimal combination principle, wherein the efficient petroleum hydrocarbon degradation flora can be immobilizedly embedded into the ball shape, petroleum pollution impact can be buffered, and petroleum pollution biodegradation efficiency can be increased.

Description

technical field [0001] The invention relates to a method for screening and fixing petroleum hydrocarbon degrading bacteria, in particular to a technical method for screening and fixing high-efficiency petroleum hydrocarbon degrading bacteria for repairing marine oil polluted environment, which belongs to the technical field of environmental restoration. Background technique [0002] In recent years, with the vigorous exploitation of seabed oil resources and the increase in crude oil trade, marine oil spill accidents have occurred frequently, causing the marine environment to face unprecedented pressure. Regardless of whether the oil spill accident occurs on the surface or bottom of the ocean, after removing most of the oil pollution by physical and chemical methods, some unrecoverable oil will remain in the ocean, which will endanger the natural marine ecological environment. Therefore, it is necessary to take necessary measures to restore the ecological environment. [000...

AI Technical Summary

Problems solved by technology

[0004] In view of the problems existing in the prior art, the object of the present invention is to scientifically and rationally determine the optimal combination scheme of highly efficient petroleum hydrocarbon degrading bacteria on the basis of screening highly efficient petroleum hydrocarbon degrading bacteria, and to overcome the problem that it cannot grow in natural sea areas