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Method for screening plasticizer

A plasticizer and screening technology, which is applied in the field of rapid screening of plasticizers, can solve problems such as failure to meet market demand, carcinogenic reproductive system, long R&D cycle, etc., to improve operational efficiency, shorten time, and test costs Reduced effect

Inactive Publication Date: 2013-09-18
WUXI ZODOLABS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The poisonous clouding agent incident broke out in Taiwan in 2011. The cause was that the manufacturer used palm oil in the clouding agent as a food additive, which was cheaper and had a longer shelf life, but it would cause cancer and mutations in the reproductive system to the human body. Replacement of industrial raw material plasticizer
Immunoassay testing technology is a method that can use color reaction for detection, but due to the long development cycle, it cannot meet the market demand in a short time

Method used

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  • Method for screening plasticizer
  • Method for screening plasticizer
  • Method for screening plasticizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment one certain brand 46 degree liquor

[0061] Use 2.5mL methanol and 2.5mL water to activate the solid-phase extraction column (filler is PLS) in sequence, take the liquor directly in four times, and put 2.5mL on the column each time. After loading the sample, wash the column with 2.5 mL of acetone solution, and discard all effluent. Use 2.5mL1 / 1 ethanol / ethyl acetate to elute the plasticizer in the solid phase extraction column, and collect the eluted liquid. The flow rate of the liquid in the above steps is one drop every 2-3 seconds. The eluted liquid is stratified, suck the lower layer, and pay attention to minimize the loss of the upper layer. Then dry the upper layer liquid using a sample concentrator at a temperature of 60° C. for 10 to 15 minutes. Take 20 microliters of ethyl acetate to redissolve the dried sample, making sure to dissolve all the substances on the wall. Take 1 microliter of the reconstituted sample and spot the plate, each take 1 mic...

Embodiment 2

[0062] Embodiment two certain brand 38 degree liquor

[0063] Use 2.5mL methanol and 2.5mL water to activate the solid-phase extraction column (filler is PLS) in sequence, take the liquor directly in four times, and put 2.5mL on the column each time. After loading the sample, wash the column with 2.5 mL of 20% methanol in water, and discard all the effluent. Use 3.5 mL of dichloromethane to elute the plasticizer in the solid-phase extraction column, and collect the eluted liquid. The flow rate of the liquid in the above steps is one drop every 2-3 seconds. The eluted liquid is stratified, suck the lower layer, and pay attention to minimize the loss of the upper layer. Then the upper liquid was blown dry using a sample concentrator at a temperature of 60° C. for 5 minutes. Take 20 microliters of ethyl acetate to redissolve the dried sample, making sure to dissolve all the substances on the wall. Take 1 microliter of the reconstituted sample and spot the plate, each take 1 mi...

Embodiment 3

[0064] Embodiment three certain brand 52 degree liquor

[0065] Use 2.5mL methanol and 2.5mL water to activate the solid-phase extraction column (filler is PSA) in sequence, and take samples directly in four times, each time 2.5mL is loaded on the column. After loading the sample, wash the column with 2.5 mL of 5% ethanol aqueous solution, and discard all the effluent. Use 2.5mL ethyl acetate to elute the plasticizer in the solid-phase extraction column, and collect the eluted liquid. The flow rate of the liquid in the above steps is one drop every 2-3 seconds. The eluted liquid is stratified, suck the lower layer, and pay attention to minimize the loss of the upper layer. Then dry the upper layer liquid using a sample concentrator at a temperature of 60° C. for 10 to 15 minutes. Take 20 microliters of ethyl acetate to redissolve the dried sample, making sure to dissolve all the substances on the wall. Take 1 microliter of the reconstituted sample and spot the plate, each t...

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Abstract

The invention relates to a method for screening a plasticizer. The method comprises the following steps of: (1) activating a solid-phase extraction column; (2) enriching the plasticizer in a to-be-tested sample by the activated solid-phase extraction column; (3) washing the solid-phase extraction column; (4) eluting the plasticizer from the solid-phase extraction column; (5) concentrating an eluent to dry by a sample concentrator and dissolving the eluent by a solvent; and (6) implementing TCL (Thin Layer Chromatography) point-plate comparison between a liquid dissolved in the step (5) and a plasticizer standard, and judging whether the plasticizer is contained. The method achieves a detection limit of 0.3mg / kg and breaks through the limitation that a large-sized instrument is needed by detection in the past; the method has the advantages that analysis cost is low, detection is sensitive, recovery rate is high, the operation is simple, accuracy rate of an experiment result is high, rapid detection on site can be achieved, requirements on operators are low, and popularization and application are convenient.

Description

technical field [0001] The invention relates to a plasticizer screening method, in particular to a plasticizer rapid screening method. Background technique [0002] Plasticizer, or plasticizer, plasticizer, is an additive that increases the softness of a material or the liquefaction of a material. Its added objects include plastics, concrete, wall materials, cement and plaster, etc. There are more than a hundred types of plasticizers, but the most commonly used ones are phthalate plasticizers. Eating large amounts may affect the liver and kidneys, and even cause cancer. [0003] Phthalate ester plasticizers are classified as suspected environmental hormones. Their biological toxicity is mainly estrogenic and anti-androgenic, which can cause endocrine disorders and hinder the reproductive function of organisms, including reduced reproductive rate, miscarriage, and birth defects. , Abnormal sperm count, testicular damage, can also cause malignant tumors or cause deformed chi...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/34G01N30/08G01N30/02
Inventor 卞禹卜叶帮伟杨晓慧李林
Owner WUXI ZODOLABS BIOTECH
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