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Method for preparing, preserving and using silicon plate cured with hyaluronic acid

A technology of hyaluronic acid and preservation method, applied in the application field of bioactive materials, can solve the problems of low adhesion, difficult application, and easy loss of biological activity of hyaluronic acid film.

Active Publication Date: 2013-09-18
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been some reports on the immobilization of hyaluronic acid on solid materials such as silicon surfaces, but hyaluronic acid films prepared by previous methods either tend to lose their biological activity, or have low adhesion to cell surfaces or other solid surfaces , not easy to apply in practice

Method used

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  • Method for preparing, preserving and using silicon plate cured with hyaluronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Preparation of a silicon plate with a high degree of hydration polymer film

[0018] The silicon plate cured with hyaluronic acid described in the present invention is to carry out functional transformation on the surface of hyaluronic acid and silicon substrate by means of chemical modification, and anchor the hyaluronic acid polymer on the substrate interface. The polymer layer thus formed will exhibit a ring, brush or spread structure. By silanizing the surface of silica, the carboxyl group of hyaluronic acid can form an amide bond with the amino group on the surface of the base layer to be anchored on the surface of the base layer. One preparation method is as follows:

[0019] First place the wafer in piranha solution (hydrogen peroxide H 2 o 2 : concentrated sulfuric acid H 2 SO 4 =3:7) Soak for 5 hours and then rinse with deionized water several times until the silicon surface is completely clean, ensuring that the surface is free of any substances...

Embodiment 2

[0026] Use a molecular weight of 10 6 The hyaluronic acid molecules are fixed on the surface of the silicon substrate by the method of the present invention, so that a layer of highly hydrated polymer hyaluronic acid layer is formed on the surface of the silicon substrate. The applicant has found in long-term research that under solution conditions, if there are no other external ions, or when there are only monovalent cations, such as hydrogen ions, sodium ions, potassium ions, etc., the silicon plate prepared by the present invention is combined with another silicon plate. When they are close to each other in the aqueous solution environment, when the distance between them reaches a certain nanometer scale, about 200-400 nanometers, the two plates begin to repel each other, and the closer the repulsion between them is, the greater the repulsion force will be. The repulsion strength is about F / R>0.2mN / m. But when 1mM MgCl is added to the solution 2 Or similar divalent catio...

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Abstract

The invention relates to a method for preserving and using a silicon plate cured with hyaluronic acid. The silicon plate cured with hyaluronic acid is placed in a solution containing divalent or higher-valence free metal cations for preservation. Silicon is used as a substrate for chemical modification, a high-water-content hyaluronic acid layer is prepared, and a solution formula using divalent or multivalent cations as a main ingredient is prepared. When the hyaluronic acid layer is immersed in the formula solution, cations in the solution can promote approaching of neighboring macromolecular chains, the overall intermolecular force can be changed, and even the total acting force is transferred from repulsive force to attraction, so that more HA (hyaluronic acid) chains can be associated, and finally the bonding property of the silicon plate cured with hyaluronic acid is improved.

Description

technical field [0001] The invention belongs to the technical field of application of bioactive materials, and in particular relates to a method for preparing, storing and using a silicon plate solidified with hyaluronic acid, that is, a divalent or higher-valent metal cation solution for a silicon plate fixed with a hyaluronic acid polymer Preservation is also carried out in an environment with divalent or higher valent metal cation solutions when in use. Background technique [0002] Hyaluronic acid (HA) is a charged biopolymer with simple primary structure, complex conformation and high viscoelasticity. It is the main component of the extracellular matrix, has special chemical properties, and participates in the biological functions of promoting cell adhesion, balancing cell penetration, and reducing friction between tissues such as joint synovium and eye vitreous. The functional properties of HA are fundamentally derived from its primary structural simplicity and chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B41/46
Inventor 姜磊杨丽敏李友训
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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