Target protein for improving spermatogensis

Abstract

The invention relates to a target protein which takes a betaB2 crystal protein to improve the spermatogensis and a novel application thereof. A betaB2 crystal protein knockout mouse model is used for analyzing the expression of the betaB2 crystal protein in mouse testis tissues to confirm the reduction of CaMKIV expression and activity, caused by betaB2 crystal protein knockout, so as to reduce a reproductive function; and a CaMKIV expression level is reduced so that the expression of Bcl-2 is reduced to enable testis germ cells to be excessively proliferated and apoptotic and damage sperms. Therefore, the target protein for improving the spermatogensis is the betaB2 crystal protein, and a novel medicine resource and a novel application preparation are provided; and the target protein has the very important value for improving the existing medical level. The target protein disclosed by the invention is safe and cheap, effective in treatment and stable in property, is suitable for large-scale production and business application of industries of medicines, reagents and the like, is easy to popularize and apply, has a very good application prospect, and has obvious social benefits and economic benefits.

Description

technical field [0001] The present invention relates to the technical fields of medicine, food, reagents, etc., specifically to a new application of crystal protein, more specifically to a target protein for improving spermatogenesis and its new application, and more specifically Said to be a crystal protein involving βB2 (or: βB 2 , betaB2, crybb 2 or crybb2) can be used as a target protein for improving spermatogenesis and its new application. Background technique [0002] βB 2 Advances in research on the functions of crystallins in and out of the lens [0003] Crystallins are usually expressed in the lens of mammalian eyes. They are the main structural proteins in the cytoplasm of the mammalian lens and play an important role in maintaining the lens' ability to refract and transmit light. According to its migration ability in the electric field, it can be divided into three categories: α, β, and γ (Graw J. Genetics of crystallins: cataract and beyond. Exp Eye Res, 200...

AI Technical Summary

Problems solved by technology

In 2007, Duprey et al. studied the expression characteristics of βB2 crystallin in mouse testis: when the mouse was just born, βB2 crystallin was expressed in the testis, but the expression level was low. As the mouse matured, its expression The amount gradually increased, maintaining a high level of expression in the mature testis; and it was found that the Swiss Webster male mice with βB2 crystal protein gene mutations had reduced fertility, which manifested as a decrease in the number of sperm in the testis and a decrease in sperm motility, while in βB2 crystal protein The C57BL / 6 strain mice with protein gene mutations were not found to have significantly reduced fertility (Duprey KM, Robinson KM, Wang Y, et al.Subfertility in mice harboring a mutation in betaB2-crystallin.Mol Vis2007;13:366- 373.)

However, according to literature search, so far, no reports have been found on the use of βB2 crystal protein as the target protein for improving spermatogenicity or the production of products that improve spermatogenic ability with the βB2 crystal protein as the target protein.

Images