Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing hybrid triploid seeds of golden trout and arctic charr

A triploid and red dot technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of chemical sensitivity, high water quality requirements for growth environment, and absence of 6-dimethylaminopurine

Inactive Publication Date: 2013-09-11
BEIJING FISHERIES RES INST
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growth environment and are particularly sensitive to chemical substances, So far, there is no successful report on the application of 6-dimethylaminopurine in the induction of triploid or hybrid triploid in salmon and trout

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Method for preparing hybrid triploid seed of golden trout and arctic char (1)

[0028] By means of water stimulation, light control, and enhanced compound feed for broodstock, the broodstock of golden trout and broodstock of arctic charr can develop and mature synchronously. The water temperature for broodstock breeding of golden trout was 10°C, and the water exchange volume in the running pool was 2h / time; the broodstock breeding water temperature for arctic char was 8°C, and the water exchange volume in the running pool was 5h / time.

[0029] The eggs are fertilized by dry method. Squeeze the roe of mature golden trout females into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. 8°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for treatment.

[0030]...

Embodiment 2

[0032] Example 2 Method for preparing hybrid triploid seed of golden trout and arctic char (2)

[0033] By means of water flow stimulation, light control, and enhanced compound feed for broodstock, the broodstock of golden trout and arctic charr can develop and mature synchronously. The water temperature for broodstock breeding of golden trout was 13°C, and the water exchange rate in the running pool was 2h / time.

[0034] The eggs are fertilized by dry method. Squeeze the roe of mature golden trout females into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. 8°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for processing.

[0035] After 13 minutes of fertilization, the fertilized eggs were quickly moved into the 6-DMAP solution with a concentration of 170mg / L an...

Embodiment 3

[0037] Example 3 Method for preparing hybrid triploid seed of golden trout and arctic char (3)

[0038]By means of water flow stimulation, light control, and enhanced compound feed for broodstock, the broodstock of golden trout and arctic charr can develop and mature synchronously. The water temperature for broodstock breeding of golden trout was 11°C, and the water exchange rate in the running pool was 2h / time.

[0039] The eggs are fertilized by dry method. Squeeze the roe of mature golden trout females into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. 7°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for processing.

[0040] After 14 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concentration of 190mg / L and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing hybrid triploid seeds of golden trout and arctic charr. The method includes: subjecting ova of female parent golden trout and sperms of male parent arctic charr to external fertilization, moving fertilized ova to a six-DMAP (N6-dimethyladenine) solution with concentration ranging from 170mg / L to 190mg / L and the temperature ranging from 9 DEG C to 11 DEG C after the fertilized ova are fertilized for 12-14 minutes, soaking for 14-16 minutes prior to moving into water at the temperature of 6-8 DEG C for hatching, and obtaining the hatched hybrid triploid larva fishes from the fertilized ova after about 50 days. During hatching, the eyeing rate of the fertilized ova is larger than 78%, the seed hatching rate reaches more than 90%, the obtained larva fishes are detected by a flow cytometer, and the triploid rate reaches 95%. The method for preparing the hybrid triploid seeds of the golden trout and the arctic charr remits the problems about sexual precocity, size miniaturization, disease breeding and the like of individuals during salmon and trout cultivation, and has the advantages of hybridization and triploid and a good marketing value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a preparation method for hybrid triploid seed of golden trout and Arctic char. Background technique [0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings with fas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com