Rapid propagation and standardized cultivation method of ampelopsis grossedentata
A cultivation method and a technology of Maoyanberry are applied in the field of agricultural cultivation to achieve the effects of increasing yield, reducing damage to natural resources, and protecting an excellent ecological environment.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0034] Material: Maoyanberry with bud rhizomes as explants
[0035] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Maoyanberry collected from Zhangjiajie, Hunan, were used as culture materials, washed with detergent and tap water, rinsed under running water for 30 minutes, and placed Treat in 75% alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 10 minutes, rinse with sterile water, cut off the discolored part of the explant and set it aside.
[0036] 2. Germination of buds: Inoculate the sterilized buds on MS+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.
[0037] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to MS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster shoots are formed, the...
Embodiment 2
[0047] Material: Maoyanberry with bud rhizomes as explants
[0048] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Maoyanberry collected from Zhangjiajie, Hunan, were used as culture materials, washed with detergent and tap water, rinsed under running water for 20 minutes, and placed in Treat in 75% alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 10 minutes, rinse with sterile water, cut off the discolored part of the explant and set it aside.
[0049] 2. Germination of buds: Inoculate the sterilized buds on MS+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.
[0050] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to MS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster shoots are formed, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com