Rapid propagation and standardized cultivation method of ampelopsis grossedentata
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A cultivation method and a technology of Maoyanberry are applied in the field of agricultural cultivation to achieve the effects of increasing yield, reducing damage to natural resources, and protecting an excellent ecological environment.
Inactive Publication Date: 2015-06-24
JISHOU UNIVERSITY
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Problems solved by technology
[0008] So far, there have been no reports on tissue culture rapid propagation and simulated natural environment cultivation of Maoyanberry at home and abroad.
Method used
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Embodiment 1
[0034] Material: Maoyanberry with bud rhizomes as explants
[0035] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Maoyanberry collected from Zhangjiajie, Hunan, were used as culture materials, washed with detergent and tap water, rinsed under running water for 30 minutes, and placed Treat in 75% alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 10 minutes, rinse with sterile water, cut off the discolored part of the explant and set it aside.
[0036] 2. Germination of buds: Inoculate the sterilized buds on MS+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.
[0037] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to MS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster shoots are formed, the...
Embodiment 2
[0047] Material: Maoyanberry with bud rhizomes as explants
[0048] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Maoyanberry collected from Zhangjiajie, Hunan, were used as culture materials, washed with detergent and tap water, rinsed under running water for 20 minutes, and placed in Treat in 75% alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 10 minutes, rinse with sterile water, cut off the discolored part of the explant and set it aside.
[0049] 2. Germination of buds: Inoculate the sterilized buds on MS+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.
[0050] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to MS+6-BA0.5mg / L+IAA0.4mg / L medium until cluster shoots are formed, ...
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Abstract
The invention provides a rapid propagation and standardized cultivation method of ampelopsis grossedentata. The rapid propagation and standardized cultivation method comprises the following steps of: reproducing a wild ampelopsis grossedentata original branch by a tissue culture method to obtain an ampelopsis grossedentata seedling; carrying out cultivation and management on the ampelopsis grossedentata seedling; carrying out field cultivation and management method on the ampelopsis grossedentata. The method disclosed by the invention has the advantages of high propagation speed, short production period, simple equipment, small occupied area, manpower and material resources conservation, convenience in industrial production, high budding speed, high propagation rate, simple method, low production cost, volume production and high application value; the production of the ampelopsis grossedentata can be carried out under an artificial control condition, is not restricted by factors including season and weather conditions, and soil and the like; the influences of invasion of plant diseases and insect pests, and pesticide residues can be removed and the quality of the ampelopsis grossedentata can be strictly controlled; the ampelopsis grossedentata seedling does not need to be additionally cultivated so that the planting cost of the ampelopsis grossedentata is greatly reduced, the yield of ampelopsis grossedentata leaves is raised, and the economical benefit is remarkable; the ampelopsis grossedentata seedling obtained by the tissue culture and propagation technology can be artificially cultivated to obtain ampelopsis grossedentata finished products with small individual difference and uniform quality; genetic resources of the ampelopsis grossedentata can be preserved; meanwhile, a wild resource of the ampelopsis grossedentata can be easily protected and the damages to a natural resource are reduced.
Description
technical field [0001] The invention relates to a method for managing Maoyan berry from rapid propagation of seedlings to long-term standardized cultivation and management, and belongs to the technical field of agricultural cultivation. Background technique [0002] Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total drugs. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collection and consumption exceeds the regeneration capacity of natural resources, it will inevitably lead to endangered or even extinction of species. Simultaneously along with the day by day destruction of natural ecol...
Claims
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Application Information
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