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Garcinia mangostana extractive, as well as preparation method and application thereof

A technology of extract and mangosteen, which is applied to mangosteen extract and its preparation method and application fields in immunosuppressants and medicines, can solve the problem that the occurrence and development of diseases cannot be effectively and fundamentally controlled, and the long-term continuous application can not be solved. , large toxic and side effects, etc., to achieve the effect of easy modification, simple structure and easy operation

Inactive Publication Date: 2013-09-04
曹绍平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first three are immunosuppressants that have been used clinically. Although the curative effects of these drugs have been proven, they can only improve symptoms, and cannot effectively control the occurrence and development of the disease fundamentally. They also have large toxic and side effects and cannot be used continuously for a long time
Antibody immunosuppressants can specifically act on lymphocytes, but at the same time they are expensive and cause serious adverse reactions, which need further improvement

Method used

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  • Garcinia mangostana extractive, as well as preparation method and application thereof
  • Garcinia mangostana extractive, as well as preparation method and application thereof
  • Garcinia mangostana extractive, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Extraction and purification of mangosteen extract

[0025] Weigh the dried mangosteen bark, crush it to 50-100 mesh, percolate with 75% ethanol after crushing, and concentrate under reduced pressure to obtain the mangosteen ethanol extract; suspend the mangosteen ethanol extract in water and then add petroleum ether , ethyl acetate and n-butanol extraction to obtain petroleum ether extract, ethyl alcohol extract, n-butanol extract and water extract respectively. Chromatography on a silica gel column on the part of ethyl acetate, eluted with a gradient of petroleum ether: acetone (3:1 ~ 1:1), for example: 3:1, 2:1, 1:1, wherein petroleum ether: acetone = 1 : The precipitated crystals in the 1 fraction are filtered by suction and rinsed with 95% ethanol, collected, recrystallized by 95% ethanol, filtered by suction, dried under reduced pressure at 55°C, and the white powder obtained is the mangosteen extract. The mangosteen extract can be directly prepared ...

Embodiment 2

[0027] Example 2: Non-specific lymphocytotoxicity test

[0028] The Balb / c mice were sacrificed by enucleation of eyeballs, bloodletting and neck dislocation, and the spleen was taken, and the cell suspension was made with RPMI1640 culture medium. Then 100 μl of cell suspension and 100 μl of drugs of various concentrations were added to the 96-well plate; the control group was added with 100 μl of culture solution containing 15% serum, and cultured at 37° C. and 5% carbon dioxide for 68 hours. At the end of the culture, add 20 μl CCK-8 to each well and continue to incubate in the incubator. After 4 hours, read the OD450 with a microplate reader.

[0029] Analysis of experimental data shows that the mangosteen extract of the present invention has certain toxicity to lymphocytes of normal mice at high concentrations, and the toxicity to lymphocytes is very weak below the concentration of 20.76 μM, while CsA is within the same concentration range. Severely affects the survival ...

Embodiment 3

[0030] Example 3: Inhibitory effect of mangosteen extract on Con A-induced proliferation of mouse T lymphocytes

[0031] Balb / c mice were sacrificed by enucleation of eyeballs, bloodletting and neck breaking, and the spleen was taken, and cell suspension was made with RPMI1640 culture medium.

[0032] Then add 90 μl of cell suspension, 100 μl of various concentrations of drugs and 10 μl of ConA to the 96-well plate; add 100 μl of culture medium containing 15% serum to the control group, and culture at 37°C and 5% carbon dioxide for 20, 44 and 68 hours . At the end of the culture, add 20 μl CCK-8 to each well and continue to incubate in the incubator. After 4 hours, read the OD450 with a microplate reader.

[0033] Analysis of experimental data shows that: the mangosteen extract of the present invention can significantly inhibit the proliferation of T cells induced by Con A (see figure 2 ), and the inhibitory effect is dose- and time-dependent, that is, the higher the dose ...

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Abstract

The invention relates to a garcinia mangostana extractive and an application thereof in immunosuppressant and medicine; the garcinia mangostana extractive provided by the invention is applied to immunosuppressant, and is an immunosuppressant with natural activity, the immunosuppressant effect of the garcinia mangostana extractive is slightly weaker than that of cyclosporin A, and is an immunosuppressant widely used at present, but the garcinia mangostana extractive has poisonousness which is lower than that of cyclosporin A in animal level, namely, the effect level of the garcinia mangostana extractive is far less than toxic level, and the garcinia mangostana extractive is an efficient and harmfulless immunosuppressant; and meanwhile, the molecular weight is small, the structure is simple, transformation and decoration are easy, and the garcinia mangostana extractive can be prepared to be different pharmaceutic preparations.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its application in the field of immunosuppressive preparations, in particular to a mangosteen extract, its preparation method and its application in immunosuppressants and medicines. Background technique [0002] Mangosteen is a plant of Garcinia genus Garcinia, and its bark (inner bark) is used as medicine. As a kind of local medicine in Southeast Asia, mangosteen has been widely used in the traditional medicine of Thailand, India, Myanmar and other countries to treat diarrhea, malaria, anti-inflammation, anti-ulcer, and anti-leukemia and septicemia. Anti-allergy and asthma and other uses. But the effect of mangosteen extract on immunosuppression is rarely reported. [0003] Immunosuppressant is a class of drugs with immunosuppressive effect, the main function is to suppress the abnormal immune response of the body, it is used in autoimmune diseases and allergic reactions and after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P19/02A61P29/00A61P37/06A61P37/02A61P37/08A61K129/00
Inventor 曹绍平岑举人
Owner 曹绍平
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