A kind of tubulin depolymerization agent polypeptide and its application

A tubulin and depolymerizing agent technology, applied to tubulin depolymerizing agent polypeptide 1 and its application field, can solve the problems of lack of specificity of small molecule depolymerizing agents, and achieve the effects of increasing the survival rate and improving the survival rate.

Inactive Publication Date: 2015-12-02
河北佑仁生物科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many successful small-molecule depolymerization agents can inhibit the activity of tubulin at the nanomolar level, but small-molecule depolymerization agents lack specificity. If long-term use of tubulin depolymerization agents lacking specificity in chronic diseases may cause produce major side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Effect of tubulin depolymerizer polypeptide 1 on tubulin polymerization and depolymerization in vitro.

[0018] Take fresh bovine brain tissue, peel off the meninges and large blood vessels, cut into pieces, wash 1-2 times with cold MES buffer, add MES buffer at a ratio of 0.5-1ml per gram of brain tissue, and at 4°C, use electric Homogenize with a homogenizer; centrifuge at 105,000 g for 1 h at 4°C, take the supernatant, add an equal volume of microtube polymerization buffer, and incubate in a water bath at 37°C for 30 min. Centrifuge at 105,000 g at 26°C for 1 hour, take the precipitate, add about 1 / 10 of the homogenate volume of cold MES buffer, stir gently or crush the precipitate with a homogenizer; place the suspension in an ice bath for 30 min to dissolve the precipitate completely. Protein content was determined by Lowry's method (SDS polyamide gel electrophoresis). Tubulin was diluted to 4-5 mg / ml with MES buffer and stored in liquid nitrogen. Take the frozen...

Embodiment 2

[0022] IC50 of tubulin depolymerizer polypeptide 1 on the growth and survival of tumor cells cultured in vitro.

[0023] The MTT colorimetric method was used. The logarithmic growth of U937 cells was added to a 96-well culture plate at 1.0×105, and cultured for 24 hours. Different concentrations of the experimental drug tubulin depolymerizer polypeptide 1 and the positive control drug Changchun Xin were added to the experimental wells and positive drug control wells, respectively. base; add the same volume of solvent to the blank group. Set up five duplicate wells per well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and incubate at 620nm in a microplate reader Measure the absorbance A value, according to the formula tumor cell growth inhibition rate=(1-absorbance value of the experimental group / absorbance value of the control group)×100%. The IC50 of the experimental drug was...

Embodiment 3

[0025] In vivo activity of tubulin depolymerizer polypeptide 1 was tested in a tumor model.

[0026] The U937 tumor model was established, and the positive control drug was vincristine; the same volume of solvent was added to the blank group, and 3 doses were set to the experimental group: 0.75, 1.5 μM, and 3 μM mg / Kg. After 21 days, the number of surviving mice was observed, and the survival rate was calculated. The results showed that the tubulin depolymerizing agent polypeptide 1 could effectively protect mice and improve the survival rate of tumor-bearing mice, with the survival rate reaching 73.2%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to tubulin depolymerizing agent polypeptides and application thereof, particularly polypeptides capable of inhibiting polymerization of tubulin and treating acute lymphatic leukemia. The invention is characterized in that the sequence of the tubulin depolymerizing agent polypeptides 1 is CRALERLV disclosed as SEQ NO.1. The invention also relates to application of the tubulin depolymerizing agent polypeptides 1 in preparation of medicines for treating acute lymphatic leukemia. A solid-phase synthesis method is utilized to chemically synthesize the tubulin depolymerizing agent polypeptides 1; and the polypeptides with the bran-new sequence can inhibit tubulin in vitro and treat acute lymphatic leukemia.

Description

technical field [0001] The present invention relates to tubulin depolymerizing agent polypeptide 1 and its application, in particular to a polypeptide capable of inhibiting tubulin polymerization and treating acute lymphocytic leukemia. Background technique [0002] Acute lymphoblastic leukemia (ALL) is a progressive malignant disease characterized by large numbers of immature white blood cells that resemble lymphoblasts. These cells can be found in the blood, bone marrow, lymph nodes, spleen and other organs. Acute lymphoblastic leukemia accounts for 80% of acute leukemia in children, and the peak incidence is between 3 and 7 years old. ALL can also occur in adults and accounts for 20% of all adult leukemias. In recent years, with the deepening of medical research, great progress has been made in the understanding and treatment of acute lymphoblastic leukemia. Among them, tubulin plays an important role in acute lymphoblastic leukemia. [0003] Microtubules are the main...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P35/02
Inventor 寇佳琳李红臣沈小勇李玖霞葛俊男
Owner 河北佑仁生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap