Ejecting-type jet flow cavitation generator and cavitation cell disruption device

Abstract

The invention discloses an ejecting-type jet flow cavitation generator and a cavitation cell disruption device. The cavitation generator comprises a nozzle, a suction chamber, a throat pipe, a diffuser and an outlet pipe. The nozzle is connected in the suction chamber in a sleeved manner, and a suction pipe is arranged on the side of the suction chamber. The cavitation cell disruption device comprises a container, a high-pressure centrifugal pump and the above cavitation generator, wherein the container is used for storing cells to be disrupted, the high-pressure centrifugal pump is connected with the container through a water pipe, and the cavitation generator is connected with the high-pressure centrifugal pump through the pipe. Valves are respectively arranged on an entrance of the nozzle, the suction pipe and the outlet pipe to control degree of cavitation. In the cavitation cell disruption device, high-pressure liquid sucks up low-pressure liquid to cause cavitation to happen in the tail end of the suction chamber and the throat pipe, cells are disrupted by microjet and shock waves produced from bubble collapse, and drastic shearing actions between the other two fluids can also have tearing effect to the cells. The ejecting-type jet flow cavitation generator and the cell disruption device for cavitation cells are simple in structure, low in cost, free of moving components, reliable in operating, strong in cavitation transport, high in crushing efficiency, and thereby applicable to mass application.

Description

[0001] technical field [0002] The invention belongs to the technical field of cell crushing, and in particular relates to a brand-new injection jet cavitation generator and a cavitation cell crushing device. Background technique [0003] Currently, cell disruption methods mainly include mechanical disruption, chemical and biochemical osmosis, and physical osmosis. [0004] The mechanical crushing method also includes high-pressure homogenization, bead milling, impact crushing, and ultrasonic crushing. It is characterized by large processing capacity, high crushing efficiency, and fast speed. It mainly relies on homogenization and grinding; during the process, the cell wall cannot Broken by strong mechanical shear force, resulting in cytoplasmic efflux. However, heating is the main problem in the mechanical fragmentation method, because the local high temperature generated by friction and impact will lead to the deterioration of the required substances in the cytoplasm. ...

AI Technical Summary

Problems solved by technology

The latter is suitable for relatively fragile bacteria, but repeated freezing and thawing will denature the protein, thereby affecting the recovery rate of active protein

[0007] At present, some scholars have proposed the concept of applying the cavitation method to cell fragmentation, but it has been difficult to popularize due to the limitation of the design of the cavitation generator.

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