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Novel culture medium for producing pulullan and method for fermenting and producing pulullan

A technology of pullulan polysaccharide and new culture medium, which is applied in the field of new culture medium and production technology for the fermentation of pullulan polysaccharide, can solve the problems of high production cost, cumbersome treatment process, low conversion rate, etc., and achieve reduction of extraction The effect of production cost, simple product composition and clear composition

Inactive Publication Date: 2013-08-14
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The conversion rate of the pullulan polysaccharide produced in the prior art is low, and the composition of the fermentation liquid is complex, resulting in high subsequent production costs, cumbersome processing technology, and low yield

Method used

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  • Novel culture medium for producing pulullan and method for fermenting and producing pulullan
  • Novel culture medium for producing pulullan and method for fermenting and producing pulullan
  • Novel culture medium for producing pulullan and method for fermenting and producing pulullan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The CGMCC NO.7055 slant strain was inoculated into the seed medium for shake flask activation, the rotation speed was 200 rpm, the temperature was 28°C, and the incubation time was 45 hours. The activated seed culture solution was transferred to a new seed shake flask at an amount of 3% (v / v) for expansion cultivation, the rotation speed was 200 rpm, the temperature was 28°C, and the cultivation time was 20 hours. The obtained seed liquid was connected to a 5L fermenter in an amount of 3% (v / v), and the liquid volume was 4L for fermentation and cultivation. The stirring speed was controlled at 300rpm, the pH was greater than 4, the temperature was 28°C, and the dissolved oxygen was greater than 5%. The fermentation time was 84 hours, and the fermentation yield was 102.4g / L.

[0069] The medium used in the experiment was:

[0070] Shake flask seed medium (g / L): sucrose: 100, peptone: 3, K 2 HPO 4 : 2, NaCl: 2.5, MgSO 4 ·7H 2 O: 0.4, FeSO 4 ·7H 2 O: 0.05pH6.5

[0...

Embodiment 2

[0073] The slant of CGMCC.NO.7055 strain was inoculated into the seed medium for shake flask activation, the rotation speed was 220rpm, the temperature was 28°C, and the incubation time was 42 hours. The activated seed culture solution was transferred to a new seed shake flask in an amount of 2% (v / v) for expansion cultivation, the rotation speed was 220 rpm, the temperature was 28°C, and the cultivation time was 18 hours. The obtained seed solution was connected to a 5L fermenter with a volume of 2% (v / v), and the liquid volume was 4L for fermentation and cultivation. The stirring speed was controlled at 350rpm, the pH was greater than 4, the temperature was 28°C, and the dissolved oxygen was greater than 5%. The fermentation time was 80 hours, and the fermentation yield was 106.4g / L.

[0074] The medium used in the experiment was:

[0075] Shake flask seed medium (g / L): sucrose: 100, peptone: 3, K 2 HPO 4 : 2, NaCl: 2.5, MgSO 4 ·7H 2 O: 0.4, FeSO 4 ·7H 2 O: 0.05pH6.5 ...

Embodiment 3

[0078] The CGMCC.NO.7055 strain slant was inoculated into the seed medium for shake flask activation, the rotation speed was 220rpm, the temperature was 30°C, and the incubation time was 46 hours. The activated seed culture solution was transferred to a new seed shake flask at an amount of 3% (v / v) for expansion cultivation, the rotation speed was 220 rpm, the temperature was 28°C, and the cultivation time was 22 hours. The obtained seed solution was connected to a 5L fermenter with a volume of 3% (v / v), and the liquid volume was 4L for fermentation and cultivation. The stirring speed was controlled at 400rpm, the pH was greater than 4, the temperature was 30°C, and the dissolved oxygen was greater than 5%. The fermentation time was 88 hours, and the fermentation yield was 100.4g / L.

[0079] The medium used in the experiment was:

[0080] Shake flask seed medium (g / L): sucrose: 100, peptone: 3, K 2 HPO 4 : 2, NaCl: 2.5, MgSO 4 ·7H 2 O: 0.4, FeSO 4 ·7H 2 O: 0.05, pH6.5 ...

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Abstract

The invention relates to a novel culture medium and production technique for fermenting and producing pulullan. The formula of the culture medium is as follows: sucrose is used as a carbon source, the concentration of the sucrose is 60-120g / L, urea is used as a nitrogen source, and the concentration of the urea is 2-4g / L; K2HPO4, MgSO4.7H2O, and FeSO4.7H2O are used as inorganic salts, the concentration of the NaCl is 2-4g / L, the concentration of the K2HPO4 is 3-10g / L, the concentration of the MgSO4.7H2O is 0.3-0.5g / L, the concentration of the FeSO4.7H2O is 25-100mg / L, and the pH ranges from 6 to 7. The culture medium has clear ingredients, the ingredients of the fermented product are simple, and the following extraction production cost can be lowered; and the culture medium is wide for ingredient source, easy to buy and lower in cost.

Description

Technical field: [0001] The invention belongs to the field of fermentation, and in particular relates to a new medium and a production process for fermenting and producing pullulan polysaccharide. technical background: [0002] Pullulan is a kind of extracellular water-soluble mucopolysaccharide similar to dextran and xanthan gum produced by the fermentation of Aureobasidium pullulans. It was developed by R. A special microbial polysaccharide discovered by Bauer. It is an exopolysaccharide produced by Aureobacidium pullulans. It is mainly composed of α-1,6-glucosidic bonds combined with maltose to form homopolysaccharides, that is, glucose is combined with α-1,4-glycosidic bonds to form maltotriose. The two ends are combined with another maltotriose by α-1,6-glucosidic bond, so that the polymer polysaccharide is formed by repeated connection. The ratio of α-1,4-glycosidic bonds to α-1,6-glycosidic bonds is 2:1, and the degree of polymerization (D.P) is 100-5000. Molecular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/10C12R1/645
Inventor 乔长晟李振海郝华璇李政王坤
Owner 天津北洋百川生物技术有限公司
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