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Method for integrally preparing compound amino acid by using superfine grinding enzymolysis

A compound amino acid and superfine pulverization technology, which is applied in the direction of fermentation, etc., to achieve a reasonable design effect

Active Publication Date: 2013-08-14
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the most commonly used method is the ion exchange method, and the oldest method is the precipitation method. However, there are few reports on the integrated preparation of compound amino acids by the combination of mechanochemical ultrafine pulverization and enzymatic hydrolysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Wash the earthworm raw material to remove impurities, dry it at 60°C for 2 hours, and then set it aside;

[0025] (2) Add the earthworm raw material and water obtained by drying to the constant temperature ultrafine pulverization system at a weight ratio of 1:2, and add enzymes (enzyme activity Calculated at 100000U / g), and controlled constant temperature superfine pulverization system host speed 3500r / min, temperature 55°C, enzymolysis reaction liquid pH value 7, enzymolysis time 3h;

[0026] (3) Put the earthworm raw material after ultrafine grinding and enzymatic hydrolysis into a centrifuge for centrifugation. The centrifugation conditions are: centrifugation speed 10000r / min, centrifugation time 25min, centrifugation temperature 4°C, and then take the supernatant at an inlet temperature of 240 ℃, feed concentration 9%, feed speed 25ml / min, nozzle pressure 0.3MPa, and spray drying to obtain compound amino acid powder. The residue is dried and returned to the con...

Embodiment 2

[0028] (1) Wash and remove impurities from animal hooves, dry them at 40°C for 4 hours, and then set aside;

[0029] (2) Add the earthworm raw material and water obtained by drying to the constant temperature ultrafine pulverization system at a weight ratio of 1:3, and add enzymes (enzyme activity Calculated at 100000U / g), and controlled constant temperature superfine pulverization system host speed 4000r / min, temperature 60°C, pH value of enzymolysis reaction solution 8, enzymolysis time 5h;

[0030] (3) Put the earthworm raw material after ultrafine grinding and enzymatic hydrolysis into a centrifuge for centrifugation. The centrifugation conditions are: centrifugal speed 10000r / min, centrifugation time 30min, centrifugation temperature 4°C, and then take the supernatant at an inlet temperature of 260°C. ℃, feed concentration 10%, feed speed 30ml / min, nozzle pressure 0.3MPa, and spray dry to obtain compound amino acid powder. After drying, the residue is returned to the cons...

Embodiment 3

[0032] (1) Wash the shrimp waste raw materials to remove impurities, dry them at 50°C for 3 hours, and then set them aside;

[0033] (2) Add the earthworm raw material and water obtained by drying to the constant temperature ultrafine pulverization system at a weight ratio of 1:5, and add enzymes (enzyme activity Calculated at 100000U / g), and control the speed of the host of the constant temperature ultrafine pulverization system to 3500r / min, the temperature to 40°C, the pH value of the enzymatic hydrolysis reaction solution to 6, and the enzymatic hydrolysis time to 1h;

[0034] (3) The earthworm raw material after ultrafine pulverization and enzymatic hydrolysis is put into a centrifuge for centrifugation. The centrifugation conditions are: centrifugal speed 8000r / min, centrifugation time 25min, centrifugation temperature 2°C, and then take the supernatant at an inlet temperature of 250°C. ℃, feed concentration 8%, feed speed 25ml / min, nozzle pressure 0.3MPa, and spray dryi...

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Abstract

The invention discloses a method for integrally preparing compound amino acid by using superfine grinding enzymolysis, and belongs to the technical field of a preparation method of the compound amino acid. The method comprises the following process steps of: 1) cleaning protein raw materials, removing impurities of the protein raw materials and drying for later use; (2) mixing the protein raw materials obtained in the step 1) with water; adding into a constant-temperature superfine grinding system; simultaneously adding proteolytic enzyme; and performing grinding enzymolysis on the protein raw materials; and 3) putting the materials obtained in the step 2) into a centrifugal machine for carrying out centrifugal separation; and spraying and drying supernate to obtain compound amino acid powder. The method is reasonable in design; the protein raw materials are subjected to earlier stage treatment and are treated by using a combined technology of mechanical force superfine grinding and enzymolysis; a condition of the constant-temperature superfine grinding system and a condition of the enzymolysis are reasonably controlled at the same time; and the protein raw materials are subjected to after-treatment, so that the environment-friendly low-carbon requirement is met, and the pollution and the hazard are avoided; and therefore, the prepared compound amino acid is a preferable fertilizer carrier to develop green organic agriculture.

Description

technical field [0001] The invention belongs to the technical field of preparation methods of compound amino acids, and in particular relates to a method for the integrated preparation of compound amino acids by ultrafine pulverization and enzymatic hydrolysis. Background technique [0002] Amino acid is the basic unit of protein. Amino acid is particularly important in the national economy, especially in medicine. The technology of separation and purification of amino acid has become an important technology in the field of medicine. [0003] In the 1990s, a new trend of the world industry was to develop "green chemical products" (that is, chemical products that are not harmful to the environment). Polymerized amino acid series products have emerged in the "green chemical products". Japan is the world's largest producer and exporter of amino acids, and it has been ahead of the world in the research and development of polymerized amino acids. At present, foreign polymerized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
Inventor 张均华朱练峰禹盛苗赵夫涛金千瑜
Owner CHINA NAT RICE RES INST
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