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Bacillus subtilis capable of effectively degrading vomitoxin and application of bacillus subtilis

A technology of Bacillus subtilis and deoxynivalenol, which is applied in the field of microorganisms, can solve the problems of characteristic research on the stress resistance function of unseen deoxynivalenol strains and the small number of bacillus, and achieve broad market application prospects, specific effects, and detoxification efficiency high effect

Active Publication Date: 2015-04-08
河南亿万中元生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the number of bacillus that can degrade vomitoxin obtained through screening and isolation is very small, and there are no related reports on the characteristics of bacillus degrading vomitoxin in feed and the stress resistance function of the strain

Method used

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  • Bacillus subtilis capable of effectively degrading vomitoxin and application of bacillus subtilis
  • Bacillus subtilis capable of effectively degrading vomitoxin and application of bacillus subtilis
  • Bacillus subtilis capable of effectively degrading vomitoxin and application of bacillus subtilis

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Embodiment 1

[0040] The screening of embodiment 1 bacterial strain

[0041] Specific steps are as follows:

[0042] 1) Screening of bacterial strains: screening of bacterial strains from animal intestinal contents, moldy feed, moldy food and natural environment.

[0043] Seed liquid preparation: Mix each intestinal content sample and the sample obtained from the environment with twice the volume of sterile water, place in a sterilized 18mm×180mm test tube, shake overnight; draw 0.1mL of the supernatant, respectively Mix with 0.8 mL of screening medium, shake and culture in a 15°C incubator for 12 hours to obtain a seed solution.

[0044] Wherein, the formula of screening medium is: NaH 2 PO 4 0.25g, NH 4 NO 3 1.0g, MgSO 4 .·7H 2 O0.25g, FeSO 4 0.001g, 17g agar, 2.0g glucose, add distilled water to 1000mL, pH7.0; sterilize by steam at 121℃ for 20min.

[0045] Preliminary screening of samples: add 100 μl of vomitoxin (100 μg / mL) to each seed solution, use non-inoculated screening me...

Embodiment 2

[0053] Embodiment 2 The cultivation method of Bacillus subtilis ANSB471 of the present invention

[0054] Take Bacillus subtilis (Bacillus subtilis) ANSB471 (preservation number CGMCC No.7344) 1.5mL (the concentration of live bacteria is 10 9 CFU / mL), inoculated in 80mL medium for shake flask fermentation, the fermentation temperature was 37°C, the pH value was 7.0, the rotation speed was 200r / min, and the fermentation time was 24h.

[0055] Among them, the shake flask fermentation medium is composed of the following components: tryptone 10g, yeast extract 2g, glucose 2g, beef extract 3g, sodium chloride 4g, disodium hydrogen phosphate 3g, magnesium sulfate heptahydrate 1.0g, distilled water 1000mL , pH 7.2.

[0056] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

Embodiment 3

[0057] Embodiment 3 The cultivation method of Bacillus subtilis ANSB471 of the present invention

[0058] Take Bacillus subtilis ANSB4711mL (viable bacteria concentration is 10 9 CFU / mL), inoculated in 50mL medium for shake flask fermentation culture, the fermentation temperature was 37°C, the pH value was 7.0, the rotational speed was 180r / min, and the fermentation time was 20h.

[0059] Among them, the shake flask fermentation medium is composed of the following components: tryptone 8g, yeast extract 1.5g, glucose 1.5g, beef extract 2g, sodium chloride 3g, disodium hydrogen phosphate 2.5g, magnesium sulfate heptahydrate 0.5g , Distilled water 800mL, pH 7.0.

[0060] After the fermentation, the fermentation broth was stored in a refrigerator at 4°C for later use.

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Abstract

The invention provides Bacillus subtilis ANSB471 capable of effectively degrading vomitoxin in feed and an application of the Bacillus subtilis. The invention also relates to a cultural method of the Bacillus subtilis strain and a method for applying fermentation liquor of the strain to degradation on vomitoxin in the feed. After reacting for 72 hours, degradation rate of the bacillus subtilis ANSB471 on the vomitoxin in the feed is 95%, and the degradation rate on the vomitoxin is still about 90% after subculture. The strain also can secrete amylase and is high in enzyme activity, and utilization rate of animals on starch in the feed can be increased. The Bacillus subtilis ANSB471 has high vomitoxin degradation activity, strong specificity and mild effect and can not damage nutritional ingredients in the feed.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a bacillus subtilis capable of efficiently degrading vomitoxin and its application. Background technique [0002] With the widespread concern of consumers on food hygiene and safety and the deepening of research on mycotoxins by researchers, the contamination of mycotoxins in raw materials and feeds has increasingly attracted the attention of the animal nutrition and feed industries. According to a survey, 60% to 70% of mycotoxins in China's feed and raw materials exceed the standard, and the proportion of vomitoxin exceeds the standard is close to 70%. Wang Ruojun et al. (Investigation Report on Mycotoxin Contamination of Feed and Feed Raw Materials in China, Feed Industry, 2003, 24(7): 53-54) surveyed 194 feeds and raw materials corn and wheat bran in 28 provinces and autonomous regions of my country. , the contamination rates of vomitoxin, T-2 toxin and zearalenone were 50.0%, 18.1% an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C07K1/30A23K1/16A23K1/175A62D3/02C12N9/54A62D101/20C12R1/125
Inventor 计成马秋刚赵丽红李笑樱
Owner 河南亿万中元生物技术有限公司
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