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Method for quickly breeding cyclamen by tissue culture technique

A technology of tissue culture and cyclamen, applied in the field of tissue culture, can solve problems such as difficult to master and immature technology of dividing bulbs, and achieve the effect of improving quality and survival rate and facilitating management

Inactive Publication Date: 2013-08-14
CHANGSHU JIASHENG AGRI SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also possible to use segmented bulbs and tissue culture for propagation, but because the technique of segmented bulbs is difficult to master, it has not been widely used at present, and there are relatively few studies on the use of tissue culture for asexual reproduction, and the technology is not yet mature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for rapidly propagating cyclamen by tissue culture technology, comprising the following steps:

[0020] (1) Selection and disinfection of explants: firstly, select vigorously growing and mature fresh cyclamen bulbs, peel off the dry and wrinkled scales on the outermost layer of the bulbs and the residual old roots at the base, rinse with running water for 20 minutes, and then put them into clean water After soaking for 15 minutes, irradiate for 20 minutes under the ultraviolet lamp, then transfer to the ultra-clean workbench, first soak it with 70% ethanol for 15 seconds on the ultra-clean workbench, then rinse it twice with sterile water, and then use 5% sodium hypochlorite Disinfect the solution for 10 minutes, rinse it twice with sterile water; then quickly add 3% hydrogen peroxide to sterilize it for 8 minutes, shake the beaker frequently during the soaking process, then pour out 1 liter of hydrogen peroxide liquid, rinse it with sterile water for 4 times, a...

Embodiment 2

[0027] A method for rapidly propagating cyclamen by tissue culture technology, comprising the following steps:

[0028] (1) Selection and disinfection of explants: firstly, select vigorously growing and mature fresh cyclamen bulbs, peel off the dry and wrinkled scales on the outermost layer of the bulbs and the residual old roots at the base, rinse with running water for 20 minutes, and then put them into clean water After soaking for 15 minutes, irradiate with ultraviolet light for 20 minutes, and then transfer to the ultra-clean workbench, soak it with 70% ethanol for 15 seconds on the ultra-clean workbench, then rinse it with sterile water for 3 times, and then use 5% sodium hypochlorite The solution was sterilized for 10 minutes, rinsed with sterile water for 3 times; then quickly added 3% hydrogen peroxide to soak and sterilize for 10 minutes, shake the beaker frequently during the soaking process, then pour out 1 liter of hydrogen peroxide liquid, rinse with sterile water...

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Abstract

The invention discloses a method for quickly breeding cyclamen by a tissue culture technique. The method specifically comprises the following steps: selection and disinfection of explants, material treatment, induction of callus, differentiation of bud, subculture, rooting culture, acclimatization and transplant and the like. Due to the tissue culture technique, the breeding efficiency of cyclamen is improved, and the utilization ratio of corm is further improved.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a cyclamen tissue culture method. Background technique [0002] Cyclamen, also known as rabbit ear flower, radish crabapple, Yipinguan, etc., is a perennial herb of Primulaceae cyclamen. has a history of more than 300 years. In the 18th century, Germany was the cultivation center, and later it became popular throughout Europe. As horticulturalists continued to improve the variety, they finally moved the cultivation center to the United States. And gradually become a worldwide ornamental flower, very popular among flower lovers, so it was elected as the queen of potted flowers. The ones that are cultivated are all hybrids, and there are many varieties. Widely cultivated in various temperate regions, it is a famous winter and spring greenhouse potted flower. The flowering period lasts for several months and is deeply loved by people. San Marino's selection of this "world-class" flow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H4/00
Inventor 陈勇明
Owner CHANGSHU JIASHENG AGRI SCI & TECH DEV
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