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High-sensitivity high-linearity D-D dimer detection kit and application method thereof

A detection kit and dimer technology, which is applied in the field of highly sensitive and linear D-D dimer detection kits, can solve the problem of high detection sensitivity and linearity, low detection sensitivity of low concentration samples, and detection of high concentration samples Insufficient linearity and other problems, to achieve the effect of widening the detection range, high sensitivity detection, and reducing the reaction rate

Inactive Publication Date: 2013-08-07
NINGBO RUI BIO TECH
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AI Technical Summary

Problems solved by technology

[0006] However, the existing methods are not wide in detection sensitivity and linearity. When used in clinical detection of D-D dimer, they are often constrained by insufficient linearity of high-concentration samples or low detection sensitivity of low-concentration samples.

Method used

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Embodiment Construction

[0021] Embodiments of the present invention are further described in detail below.

[0022] A highly sensitive and highly linear D-D dimer detection kit of the present invention, wherein: includes reagent 1, reagent 2, D-D dimer standard and control D-D dimer abnormal serum, reagent 1 is 30mmol / L Tris buffer. Reagent two includes large-diameter mouse anti-human D-D dimer monoclonal antibody latex particles at a concentration of 0.01% (w / v) to 0.04% (w / v) and a concentration of 0.05 (w / v) to 0.2% (w / v) / v) small diameter mouse anti-human D-D dimer monoclonal antibody latex particle mixture, the specific antibody content on the small diameter mouse anti-human D-D dimer monoclonal antibody latex particle is the large diameter mouse anti-human D-D dimer 0.5% to 10% of the specific antibody content on the monoclonal antibody latex particles.

[0023] The latex particle diameter of the large-diameter mouse anti-human D-D dimer monoclonal antibody is between 0.15 micron and 0.29 m...

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PUM

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Abstract

The invention discloses a high-sensitivity high-linearity D-D dimer detection kit and an application method thereof. A reagent one is a 30mmol / L trihydroxymethyl aminomethane buffer solution. A reagent two comprises a mixed liquid of large-diameter mouse-anti-human D-D dimer monoclonal antibody latex particles with a concentration of 0.01-0.04% (w / v) and small-diameter mouse-anti-human D-D dimer monoclonal antibody latex particles with a concentration of 0.05-0.2% (w / v). Specific antibody content in the small-diameter mouse-anti-human D-D dimer monoclonal antibody latex particles is 0.05-10% of that in the large-diameter mouse-anti-human D-D dimer monoclonal antibody latex particles. Through optimizing the average diameter of large and small particles and concentrations thereof in the reaction system, D-D dimer high-sensitivity detection under low-concentration situation can be realized, and detection range under high-concentration situation can be expanded.

Description

technical field [0001] The invention relates to the field of biological sample detection, in particular to a kit for improving the detection sensitivity and detection range of biological samples by using two types of latex particles with different average diameters, and a method for using the same, in particular to a highly sensitive, high Linear D-D dimer detection kit and method of use thereof. Background technique [0002] D-dimer (D-D dimer) is derived from cross-linked fibrin clots dissolved by plasmin. In the blood coagulation and fibrinolysis system, due to the action of the thirteenth factor of coagulation, the stable cross-linked fibrin is decomposed by plasmin, and the fibrin monomer is cross-linked by activated factor XIII, and then passed through A specific degradation product produced by plasmin hydrolysis is a specific marker of fibrinolytic process. [0003] The increase of D-dimer in the blood proves the formation of thrombus in the body and activates the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 张闻
Owner NINGBO RUI BIO TECH
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