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Preparation method of microbial nutrition agent for wastewater biochemical treatment

A biochemical treatment and microbial technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of limiting the application of nutrients, single nutritional components, high operating costs, etc., to facilitate the absorption of microorganisms, wide sources of raw materials, and treatment The effect of low cost

Active Publication Date: 2013-07-31
SHANDONG ACAD OF ENVIRONMENTAL SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above products have played a positive role in improving microbial activity, but there are problems such as single nutritional components, large dosage, and high operating costs, which limit the application of these nutrients in improving microbial activity in industrial wastewater biochemical treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of microbial nutrient for biochemical treatment of wastewater, the steps are as follows:

[0037] (1) Mix the fish meal passed through a 100-mesh sieve with distilled water to prepare a slurry with a mass concentration of 5%;

[0038] (2) Warm the slurry prepared in step (1) to 45°C, adjust the pH to 7.2 with sodium hydroxide, then add compound protease Protamex with a slurry quality of 0.5‰, alkaline lipase with a slurry quality of 0.5‰, and a slurry quality of 3 ‰ of trypsin, stirred and reacted for 4 hours to obtain an enzymolysis slurry;

[0039] (3) Phosphoric acid adjusts the pH of the enzymatic hydrolysis slurry prepared in step (2) to 1.0, and stirs for 1 hour for the acid hydrolysis reaction to obtain the acid hydrolysis slurry, then adds an equal weight of distiller's grains to the acid hydrolysis slurry, and adjusts the pH with phosphoric acid To 1.5, the microbial nutrient for wastewater biochemical treatment is prepared.

Embodiment 2

[0041] A preparation method of microbial nutrient for biochemical treatment of wastewater, the steps are as follows:

[0042] (1) Mix the fish meal passed through a 100-mesh sieve with distilled water to prepare a slurry with a mass concentration of 20%;

[0043] (2) Warm the slurry prepared in step (1) to 50°C, adjust the pH to 8.0 with sodium hydroxide, then add compound protease Protamex with a slurry quality of 1.0‰, alkaline lipase with a slurry quality of 1.0‰, and a slurry quality of 4 ‰ of trypsin, stirred and reacted for 5h to obtain enzymolysis slurry;

[0044] (3) Phosphoric acid adjusts the pH of the enzymatic hydrolysis slurry prepared in step (2) to 1.3, and stirs for 1.5 hours to carry out the acid hydrolysis reaction to obtain the acid hydrolysis slurry. The pH is adjusted to 1.8 to prepare the microbial nutrient for wastewater biochemical treatment.

Embodiment 3

[0046] A preparation method of microbial nutrient for biochemical treatment of wastewater, the steps are as follows:

[0047] (1) Mix the fish meal passed through a 100-mesh sieve with distilled water to prepare a slurry with a mass concentration of 30%;

[0048] (2) Warm the slurry prepared in step (1) to 55°C, adjust the pH to 8.5 with sodium hydroxide, then add composite protease Protamex with a slurry quality of 1.5‰, alkaline lipase with a slurry quality of 1.5‰, and a slurry quality of 5 ‰ of trypsin, stirred and reacted for 6 hours to obtain an enzymolysis slurry;

[0049] (3) Phosphoric acid adjusts the pH of the enzymolysis slurry prepared in step (2) to 1.5, and stirs for 2.0 hours to carry out the acid hydrolysis reaction to obtain the acid hydrolysis slurry, and then adds 2 times the weight of distiller's grains to the acid hydrolysis slurry, phosphoric acid The pH is adjusted to 2.0 to prepare the microbial nutrient for wastewater biochemical treatment.

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Abstract

The invention relates to a preparation method of a microbial nutrition agent for wastewater biochemical treatment, comprising the following steps: Mixing fish meal and distilled water to prepare a slurry with the mass concentration of 5-30%; (2) heating the slurry to 45-55 DEG C, adjusting pH to 7.2-8.5, adding protease and alkaline lipase, stirring and reacting for 4-6h to prepare an enzymolysis slurry; and (3) adjusting pH of the enzymolysis slurry to 1.0-1.5, stirring and carrying out an acidolysis reaction for 1-2h to prepare the acidolysis slurry, adding alcohol slops of 1-2 times the weight of the acidolysis slurry into the acidolysis slurry, and adjusting pH to 1.5-2.0 to prepare the microbial nutrition agent for wastewater biochemical treatment. The nutrition agent prepared by the preparation method of the microbial nutrition agent for wastewater biochemical treatment is used for industrial wastewater biochemical treatment. Additive dosage of the nutrition agent is little. Microbial activity is remarkably raised. Biochemical treatment efficiency is raised. And handling expense is low.

Description

technical field [0001] The invention relates to a method for preparing a microbial nutrient for biochemical treatment of wastewater, belonging to the technical field of wastewater treatment. Background technique [0002] The treatment methods of industrial wastewater mainly include physical method, chemical method, physicochemical method and biochemical method. Among them, physical method, chemical method and physicochemical method usually have disadvantages such as low removal efficiency, high treatment cost, high equipment cost, and secondary pollution. They are generally used as auxiliary treatment methods such as pretreatment or advanced treatment, and are applied in industrial wastewater. The biochemical treatment method uses the pollutants in the wastewater as the substrate, and degrades the pollutants in the wastewater through the metabolic process of microorganisms to realize the purification of the wastewater. Biochemical treatment has the advantages of high treatm...

Claims

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Application Information

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IPC IPC(8): C12N1/00C02F3/34
Inventor 洪卫刘勃邹晓凤冯晓静季华东庄会栋苏颖
Owner SHANDONG ACAD OF ENVIRONMENTAL SCI CO LTD
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