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Epsilon-polylysine-DOHA in-situ gel adhesive material and preparation method thereof

A polylysine, in-situ gel technology, applied in surgical adhesives, applications, medical science and other directions, can solve the problems of in-situ gel and preparation methods that have not yet been reported, and achieve good biodegradability, preparation Mild conditions and low cost effects

Inactive Publication Date: 2014-05-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The method of modifying the side chain of ε-polylysine with 3,4-dihydroxyphenylpropionic acid (DOHA) containing catechol groups simulates the viscous silk protein secreted by mussel biological silk, through Schiff base Reaction preparation of mussel biomimetic in situ gel bioadhesive, the in situ gel and preparation method in this aspect have not been reported yet

Method used

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  • Epsilon-polylysine-DOHA in-situ gel adhesive material and preparation method thereof
  • Epsilon-polylysine-DOHA in-situ gel adhesive material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Weigh 1 g of dextran powder with a number-average molecular weight of 100,000 and dissolve it in 80 ml of deionized water, and stir at room temperature until it is completely dissolved; weigh 790 mg of NaIO 4 The powder was dissolved in 25ml of deionized water and stirred until completely dissolved; the NaIO 4 The solution was added to the dextran solution, protected from light, and continued to stir at a stirring speed of 200 rpm for 24 h; 4395 mg of diethylene glycol was added to the mixed solution of dextran and stirred at room temperature for 1 hour; after the reaction was completed, the solution was dialyzed with deionized water as a medium for 72 hours, and lyophilized to obtain dextran aldehyde with an oxidation degree of 42%. Dissolve the dextran aldehyde sample in phosphate buffer with pH=7.2~7.4 to prepare a dextran solution with a concentration of 200mg / ml;

[0025] Weigh 1.5g of ε-polylysine powder with a number average molecular weight of 4000, dissolve it...

Embodiment 2

[0028] Weigh 2g of dextran powder with a number average molecular weight of 70,000 and dissolve it in 160ml of deionized water, stir at room temperature until completely dissolved; weigh 1.8g of NaIO 4 The powder was dissolved in 54 ml of deionized water and stirred until completely dissolved; the NaIO 4 The solution was added to the dextran solution, protected from light, and continued to stir at a stirring speed of 200 rpm for 24 h; 4 790 mg of diethylene glycol was added to the mixed solution of dextran and the mixture was stirred at room temperature for 1 hour; after the reaction was completed, the solution was dialyzed with deionized water for 72 hours, and lyophilized to obtain dextran aldehyde with an oxidation degree of 45%. Dissolve the dextran aldehyde sample in a phosphate buffer with pH=7.2~7.4 to prepare a dextran solution with a concentration of 150mg / ml;

[0029] Dissolve 1.5 g of ε-polylysine powder with a number-average molecular weight of 4000 in 65 ml of ph...

Embodiment 3

[0032] Weigh 3g of dextran powder with a number average molecular weight of 40,000 and dissolve it in 240ml of deionized water, stir at room temperature until completely dissolved; weigh 2.4g of NaIO 4 The powder was dissolved in 76 ml of deionized water and stirred until completely dissolved; the NaIO 4 The solution was added to the dextran solution, protected from light, and continued to stir at a stirring speed of 200 rpm for 24 h; 4 1.2 g of diethylene glycol was added to the mixed solution of glucan and the mixture was stirred at room temperature for 1 h; after the reaction, the solution was dialyzed with deionized water as a medium for 72 h, and lyophilized to obtain glucan aldehyde with an oxidation degree of 48%. Dissolve the dextran aldehyde sample in phosphate buffer with pH=7.2~7.4 to prepare a dextran solution with a concentration of 180mg / ml;

[0033] Dissolve 1.5 g of ε-polylysine powder with a number average molecular weight of 4000 in 60 ml of phosphate buffer...

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Abstract

The invention discloses an epsilon-polylysine-3,4-dihydroxyphenylpropionic acid (DOHA) in-situ gel adhesive material and a preparation method thereof. The in-situ gel adhesive material is composed of glucan aldehyde, 3,4-dihydroxyphenylpropionic acid modified polylysine, and water. The preparation method comprises the steps that: 3,4-dihydroxyphenylpropionic acid modified epsilon-polylysine is synthesized; with PBS as a solvent, an epsilon-polylysine-3,4-dihydroxyphenylpropionic acid solution is prepared; with PBS as a solvent, a glucan aldehyde solution is prepared; and the two solutions are uniformly mixed under a physiological pH condition, such that the in-situ gel is formed. The in-situ gel adhesive material and the method provided by the invention have the advantages that: natural biological materials are adopted; the cost is low; the process is simple; the reaction is fast and controllable; and the prepared material has good adhesion performance, biocompatibility, and biodegradability. As a biomedical tissue adhesive, the material has good application prospect in the fields of tissue adhesion, nerve anastomosis reparation, and the like.

Description

technical field [0001] The invention relates to an ε-polylysine-3,4-dihydroxyphenylpropionic acid (DOHA) in-situ gel adhesive material and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] Medical bioadhesives have developed rapidly in the past ten years. Traditional surgical suture treatment is time-consuming and prone to tissue inflammatory response, infection, hyperplasia, rupture, and even tissue and organ damage, necrosis, and non-union. Compared with surgical sutures, the advantage of medical bioadhesives is that they can reduce wound inflammation, infection, ischemic necrosis, and adhesion. At the same time, medical bioadhesives are easy to use, simple, and low in cost. The tissue toxicity and dose carcinogenicity of traditional medical adhesives such as cyanoacrylate adhesives and the low bond strength of fibrin adhesives urgently require researchers to develop and synthesize a new type of biocompa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/08A61L24/04C08G69/48C08J3/24C08J3/075
Inventor 赵瑾蒋瑞袁晓燕
Owner TIANJIN UNIV
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