Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

The preparation method of chondroitin sulfate sodium

A technology of chondroitin sulfate sodium and cartilage, applied in the biological field, can solve the problems of low content of chondroitin sulfate sodium, heavy metals, nitrogen content, residue on ignition exceeding the standard, failing to meet standards and export standards, etc. value, reduce production cost, and increase dry matter yield

Active Publication Date: 2015-09-23
CHONGQING AOLI BIOPHARM
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, sodium chondroitin sulfate adopts the alkali salt digestion and precipitation method, that is, after the cartilage is alkalized, it is enzymatically hydrolyzed and filtered, and then directly precipitated with alcohol. The content of sodium chondroitin sulfate produced by this method is low, heavy metals, nitrogen content, and burning The residue exceeds the standard and cannot meet the standards of the 2010 edition of the "Chinese Pharmacopoeia" and export standards

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The preparation method of chondroitin sulfate sodium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, preparation sodium chondroitin sulfate

[0024] One, prepare sodium chondroitin sulfate according to the following steps:

[0025] (1) Raw material pretreatment: Put cartilage tissues such as throat bones, nasal middle bones, and trachea of ​​pigs and cattle into washing jars, soak them in water until they are completely submerged, raise the temperature to about 80°C, stir and wash repeatedly, and remove meat residues, floating Oil, impurities, until the raw materials are washed.

[0026] Put the washed raw materials into the digestion tank, add purified water until the cartilage is completely submerged, and then heat the ingredients. After boiling, turn off the steam and simmer the oil for 2 hours. After the raw materials are cooked, remove all the slick oil.

[0027] (2) Enzymolysis: Cool the cooked raw materials to 30°C, add salt with 1% cartilage weight, and adjust the pH value to 8 with dilute alkali solution (40% NaOH aqueous solution: 100ml water ...

Embodiment 2

[0061] Embodiment 2, preparation sodium chondroitin sulfate

[0062] One, prepare sodium chondroitin sulfate according to the following steps:

[0063] (1) Raw material pretreatment: Put cartilage tissues such as throat bones, nasal middle bones, and trachea of ​​pigs and cattle into washing jars, soak them in water until they are completely submerged, raise the temperature to about 80°C, stir and wash repeatedly, and remove meat residues, floating Oil, impurities, until the raw materials are washed.

[0064] Put the washed raw materials into the digestion tank, add purified water until the cartilage is completely submerged, and then heat the ingredients. After boiling, turn off the steam and simmer the oil for 3 hours. After the raw materials are cooked, remove all the slick oil.

[0065] (2) Enzymolysis: Cool the cooked raw materials to 40°C, add salt with 3.5% cartilage weight, and adjust the pH value to 11 with dilute alkali solution (40% NaOH aqueous solution: 100ml wat...

Embodiment 3

[0091] Embodiment 3, preparation sodium chondroitin sulfate

[0092] One, prepare sodium chondroitin sulfate according to the following steps:

[0093] (1) Raw material pretreatment: Put cartilage tissues such as throat bones, nasal middle bones, and trachea of ​​pigs and cattle into washing jars, soak them in water until they are completely submerged, raise the temperature to about 80°C, stir and wash repeatedly, and remove meat residues, floating Oil, impurities, until the raw materials are washed.

[0094] Put the washed raw materials into the digestion tank, add purified water until the cartilage is completely submerged, and then heat the ingredients. After boiling, turn off the steam and simmer the oil for 2.5 hours. After the raw materials are cooked, remove all the slick oil.

[0095] (2) Enzymolysis: Cool the cooked raw materials to 35°C, add salt with 2% cartilage weight, and adjust the pH value to 10 with dilute alkali solution (40% NaOH aqueous solution: 100ml wat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
specific rotationaaaaaaaaaa
specific rotationaaaaaaaaaa
Bronsted acidityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of sodium chondroitin sulfate, and the preparation method comprises the following steps of: (1) carrying out enzymolysis; (2) carrying out resin absorption; (3) eluting; (4) decoloring; (5) carrying out ultrafiltration; (6) depositing; and (7) dewatering and drying. The sodium chondroitin sulfate obtained by adopting the preparation method disclosed by the invention can be used for enhancing the yield of a product dry substance by 2% and increasing the content of the sodium chondroitin sulfate by 10%, thereby enhancing the product additional value, reducing the production cost and enhancing the economic benefit.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of sodium chondroitin sulfate. Background technique [0002] Chondroitin Sulfate Sodium is a sulfated chain mucopolysaccharide sodium salt extracted from cartilage tissues such as larynx, nasal middle bone, and trachea of ​​animals. Sodium chondroitin sulfate is mainly composed of the sulfate sodium salt of the copolymer of N-acetylgalactosamine (2-acetamide-2-deoxy-β-D-galactopyranose) and D-glucuronic acid, and hexose in the copolymer Linked alternately by β-1,3 and β-1,4 glycosidic bonds. [0003] At present, sodium chondroitin sulfate adopts the alkali salt digestion and precipitation method, that is, after the cartilage is alkalized, it is enzymatically hydrolyzed and filtered, and then directly precipitated with alcohol. The content of sodium chondroitin sulfate produced by this method is low, heavy metals, nitrogen content, and burning The residue exceed...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 冉崇荣朱占权陈曾兰乾友
Owner CHONGQING AOLI BIOPHARM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com